{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T19:27:12Z","timestamp":1696188432316},"reference-count":17,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,10,25]],"date-time":"2013-10-25T00:00:00Z","timestamp":1382659200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The finding of excess urinary glycosaminoglycans (<jats:styled-content style=\"fixed-case\">GAG<\/jats:styled-content>) is the first step in the laboratory diagnosis of mucopolysaccharidosis (<jats:styled-content style=\"fixed-case\">MPS<\/jats:styled-content>). Urinary screening tests are based upon the binding of <jats:styled-content style=\"fixed-case\">GAG<\/jats:styled-content> to dimethylmethylene blue. Alternatively, paper spot tests using toluidine blue are used in human and veterinary laboratory medicine. Positive samples undergo <jats:styled-content style=\"fixed-case\">GAG<\/jats:styled-content> isolation for subsequent characterization. Here, we describe a 3\u2010year\u2010old English Cocker Spaniel with a positive urinary <jats:styled-content style=\"fixed-case\">GAG<\/jats:styled-content> test, but without other clinical signs of <jats:styled-content style=\"fixed-case\">MPS<\/jats:styled-content>. Urine samples were strongly positive with the dimethylmethylene blue test, and isolated <jats:styled-content style=\"fixed-case\">GAG<\/jats:styled-content> subjected to electrophoresis on cellulose acetate revealed a band co\u2010migrating with dermatan sulfate. However, the isolated <jats:styled-content style=\"fixed-case\">GAG<\/jats:styled-content> were resistant to digestion with chondroitinase <jats:styled-content style=\"fixed-case\">ABC<\/jats:styled-content>, suggesting that the band did not represent dermatan sulfate. This was confirmed by mobility of the isolated <jats:styled-content style=\"fixed-case\">GAG<\/jats:styled-content> different from dermatan sulfate on agarose gel electrophoresis. <jats:styled-content style=\"fixed-case\">MPS<\/jats:styled-content> types <jats:styled-content style=\"fixed-case\">VI<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">VII<\/jats:styled-content> were excluded by enzyme assay. To test the hypothesis of a nutritional source, a healthy control dog was fed the same dog food as the index case. His urine showed a comparable abnormal <jats:styled-content style=\"fixed-case\">GAG<\/jats:styled-content> screening test and electrophoretic pattern. In addition, the analysis of an algal supplement present in the administered dog food showed a similar electrophoretic <jats:styled-content style=\"fixed-case\">GAG<\/jats:styled-content> pattern. The Cocker Spaniel was not available for further testing after withdrawal of the supplement. Algae contain highly sulfated fucans and galactans, and it appears that commercial dog food containing such algal, and possibly other, supplements can give rise to false\u2010positive urinary <jats:styled-content style=\"fixed-case\">MPS<\/jats:styled-content> screening tests.<\/jats:p>","DOI":"10.1111\/vcp.12096","type":"journal-article","created":{"date-parts":[[2013,10,25]],"date-time":"2013-10-25T19:00:27Z","timestamp":1382727627000},"page":"504-507","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Interference of an algal nutritional supplement with a urinary metabolic screening test for glycosaminoglycans in a dog suspected to have a storage disease"],"prefix":"10.1111","volume":"42","author":[{"given":"Adrian C.","family":"Sewell","sequence":"first","affiliation":[{"name":"Department of Pediatrics University Children's Hospital Frankfurt am Main Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander","family":"Pankraz","sequence":"additional","affiliation":[{"name":"Biocontrol Laboratory for Veterinary Investigation Ingelheim Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,10,25]]},"reference":[{"key":"e_1_2_4_2_1","doi-asserted-by":"crossref","first-page":"1472","DOI":"10.1093\/clinchem\/35.7.1472","article-title":"Dimethylene blue\u2010based spectrophotometry of glycosaminoglycans in untreated urine: a rapid screening procedure for mucopolysaccharidoses","volume":"35","author":"DeJong JGN","year":"1989","journal-title":"Clin Chem"},{"key":"e_1_2_4_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-540-76698-8_17"},{"key":"e_1_2_4_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165x.2012.00485.x"},{"key":"e_1_2_4_5_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1025177411938"},{"key":"e_1_2_4_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)93575-1"},{"key":"e_1_2_4_7_1","first-page":"3421","volume-title":"The Metabolic and Molecular Bases of Inherited Disease","author":"Neufeld EF","year":"2001"},{"key":"e_1_2_4_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0009-9120(87)80088-7"},{"key":"e_1_2_4_9_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.29.2.111"},{"key":"e_1_2_4_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2002.tb02344.x"},{"key":"e_1_2_4_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-370491-7.00024-6"},{"key":"e_1_2_4_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2006.08.017"},{"key":"e_1_2_4_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0009-8981(97)00084-3"},{"key":"e_1_2_4_14_1","first-page":"18","volume-title":"Current Veterinary Therapy XI","author":"Giger U","year":"1992"},{"key":"e_1_2_4_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.20453"},{"key":"e_1_2_4_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/np980143n"},{"key":"e_1_2_4_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwn085"},{"key":"e_1_2_4_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2011.08.009"}],"container-title":["Veterinary Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fvcp.12096","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vcp.12096","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T21:37:37Z","timestamp":1696023457000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vcp.12096"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,10,25]]},"references-count":17,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2013,12]]}},"alternative-id":["10.1111\/vcp.12096"],"URL":"https:\/\/doi.org\/10.1111\/vcp.12096","archive":["Portico"],"relation":{},"ISSN":["0275-6382","1939-165X"],"issn-type":[{"value":"0275-6382","type":"print"},{"value":"1939-165X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,10,25]]},"assertion":[{"value":"2013-10-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}