{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T15:43:12Z","timestamp":1770219792489,"version":"3.49.0"},"reference-count":8,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,7,23]],"date-time":"2014-07-23T00:00:00Z","timestamp":1406073600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2014,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A 5\u2010year\u2010old neutered male Mastiff and an 8\u2010year\u2010old spayed female Labrador Retriever were presented to the University of Minnesota Veterinary Medical Center. The Mastiff was presented for evaluation of lameness and pyoderma one month prior in Missouri, where he tested positive for<jats:italic>Ehrlichia canis<\/jats:italic>by serum<jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>test, treated with doxycycline.<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>for<jats:italic>Ehrlichia<\/jats:italic>sp<jats:italic>, Anaplasma<\/jats:italic>sp<jats:italic>, Babesia<\/jats:italic>sp, and<jats:italic>Bartonella<\/jats:italic>sp<jats:italic>,<\/jats:italic>and<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>for antigen receptor rearrangement were negative, serum protein electrophoresis (<jats:styled-content style=\"fixed-case\">SPE<\/jats:styled-content>) revealed polyclonal gammopathy, and mildly reactive lymphoid cells were seen cytologically. The Labrador presented with a proliferative rostral mandibular gingival mass and lipomas for further presurgical evaluation of cold agglutinin activity documented by a commercial laboratory 2\u00a0years earlier prior to removal of a grade<jats:styled-content style=\"fixed-case\">II<\/jats:styled-content>mast cell tumor. This dog had a negative<jats:styled-content style=\"fixed-case\">SNAP<\/jats:styled-content>4Dx, normal<jats:styled-content style=\"fixed-case\">SPE<\/jats:styled-content>, and persistently increased serum<jats:styled-content style=\"fixed-case\">ALP<\/jats:styled-content>activity and polyuria\/polydipsia suggestive for hyperadrenocorticism. Both dogs had markedly agglutinated<jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>in the<jats:styled-content style=\"fixed-case\">EDTA<\/jats:styled-content>samples that dispersed with warming, and normal plasma color. Cold agglutinin activity was demonstrated by direct saline agglutination testing using whole blood and washed erythrocytes demonstrating agglutination at 30\u00b0C, 25\u00b0C, 15\u00b0C, and 4\u00b0C, but not at 37\u00b0C<jats:bold>.<\/jats:bold><jats:styled-content style=\"fixed-case\">CBC<\/jats:styled-content>results (<jats:styled-content style=\"fixed-case\">ADVIA<\/jats:styled-content>2120i) from the Mastiff revealed no significant differences in the<jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>results obtained at room temperature (<jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>) and at 37\u00b0C; however, the<jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>run demonstrated negative bias in neutrophil and platelet concentrations attributed to rapid<jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>settling. This uncommon hematologic condition may cause artifacts on the automated leukogram and platelet count, and may be subclinical for long periods.<\/jats:p>","DOI":"10.1111\/vcp.12173","type":"journal-article","created":{"date-parts":[[2014,7,23]],"date-time":"2014-07-23T14:19:13Z","timestamp":1406125153000},"page":"330-336","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Cold agglutinin activity in 2 dogs"],"prefix":"10.1111","volume":"43","author":[{"given":"Gabriela","family":"Rojas\u2010Temahuay","sequence":"first","affiliation":[{"name":"Clinical Pathology Veterinary and Zootechnical Faculty National Autonomous University of Mexico Mexico City Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah","family":"Crain","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences University of Minnesota St. Paul MN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine","family":"Benson","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences University of Minnesota St. Paul MN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leslie","family":"Sharkey","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences University of Minnesota St. Paul MN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Geneva","family":"Nothnagel","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences University of Minnesota St. Paul MN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,7,23]]},"reference":[{"key":"e_1_2_4_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.blre.2012.01.002"},{"key":"e_1_2_4_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.1997.tb00689.x"},{"key":"e_1_2_4_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-5827.1994.tb03279.x"},{"key":"e_1_2_4_5_1","doi-asserted-by":"crossref","first-page":"907","DOI":"10.2460\/javma.1990.196.06.907","article-title":"Cold agglutinins in cats with haemobartonellosis","volume":"196","author":"Zulty JC","year":"1990","journal-title":"J Am Vet Med Assoc"},{"key":"e_1_2_4_6_1","doi-asserted-by":"crossref","first-page":"505","DOI":"10.2460\/javma.1977.170.05.505","article-title":"Cold hemagglutinin disease in a dog","volume":"170","author":"Greene CE","year":"1977","journal-title":"J Am Vet Med Assoc"},{"key":"e_1_2_4_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.1996.tb02061.x"},{"key":"e_1_2_4_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2010.02.001"},{"key":"e_1_2_4_9_1","doi-asserted-by":"publisher","DOI":"10.1309\/LM23R7FULSTUJSJD"}],"container-title":["Veterinary Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fvcp.12173","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vcp.12173","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,3]],"date-time":"2025-05-03T22:31:23Z","timestamp":1746311483000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vcp.12173"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,7,23]]},"references-count":8,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2014,9]]}},"alternative-id":["10.1111\/vcp.12173"],"URL":"https:\/\/doi.org\/10.1111\/vcp.12173","archive":["Portico"],"relation":{},"ISSN":["0275-6382","1939-165X"],"issn-type":[{"value":"0275-6382","type":"print"},{"value":"1939-165X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,7,23]]},"assertion":[{"value":"2014-07-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}