{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T16:26:25Z","timestamp":1759335985098},"reference-count":14,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,8,28]],"date-time":"2014-08-28T00:00:00Z","timestamp":1409184000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2014,12]]},"abstract":"Background<\/jats:title>Cytologic diagnosis of canine histiocytic sarcoma (CHS<\/jats:styled-content>) can be challenging because neoplastic histiocytes commonly show marked nuclear and cellular atypia and may resemble other pleomorphic malignant round cell tumors. Therefore, even on histopathologic examination, immunostaining is often necessary for a definitive diagnosis.<\/jats:p><\/jats:sec>Objectives<\/jats:title>The objective of this study was to validate an anti\u2010human CD<\/jats:styled-content>204 antibody for immunocytochemical staining of air\u2010dried smears for a rapid definitive diagnosis of CHS<\/jats:styled-content>.<\/jats:p><\/jats:sec>Methods<\/jats:title>Cytologic specimens were obtained from 10 dogs with CHS<\/jats:styled-content> and 45 dogs with other tumors. After the cytologic evaluation of modified Giemsa\u2010stained smears, acetone\u2010fixed specimens were immunostained using mouse anti\u2010human CD<\/jats:styled-content>204 antibodies. All immunocytochemical specimens were assessed blinded and at high\u2010power magnification (\u00d7\u00a040 objective) in 10 randomly selected fields per sample. Parameters evaluated were the subjective staining intensity and location, and the proportion of positive cells.<\/jats:p><\/jats:sec>Results<\/jats:title>All 10 CHS<\/jats:styled-content> samples showed intense positive staining for CD<\/jats:styled-content>204 in \u2265\u00a050% of the cells, whereas the 45 other tumors were negative for CD<\/jats:styled-content>204 staining.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>Immunocytochemistry of air\u2010dried cytologic smears of CHS<\/jats:styled-content> for CD<\/jats:styled-content>204 is useful for a rapid confirmation of a cytologic diagnosis of CHS<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vcp.12190","type":"journal-article","created":{"date-parts":[[2014,8,28]],"date-time":"2014-08-28T15:10:41Z","timestamp":1409238641000},"page":"589-593","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Immunocytochemical detection of the class A 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