{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,11]],"date-time":"2024-08-11T11:49:44Z","timestamp":1723376984654},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,12,8]],"date-time":"2014-12-08T00:00:00Z","timestamp":1417996800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Conseil R\u00c3\u00a9gional de Basse Normandie"},{"name":"Haras Nationaux"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2015,3]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Inflammatory airway disease (<jats:styled-content style=\"fixed-case\">IAD<\/jats:styled-content>) affects performance and well\u2010being of horses. Diagnosis is primarily reached by bronchoalveolar lavage (<jats:styled-content style=\"fixed-case\">BAL<\/jats:styled-content>) cytology which is invasive and requires sedation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>The purpose of this study was to identify differential gene expression in peripheral blood of horses with<jats:styled-content style=\"fixed-case\">IAD<\/jats:styled-content>using species\u2010specific expression microarrays.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Equine gene expression microarrays were used to investigate global<jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>expression in circulating leukocytes from healthy, IAD\u2010affected, and low\u2010performing Standardbred and endurance horses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Nine genes in Standardbred and 61 genes in endurance horses were significantly differentially regulated (<jats:italic>P\u00a0<\/jats:italic>&lt;\u00a0.001). These genes were related to inflammation (eg,<jats:italic>ALOX15B<\/jats:italic>,<jats:italic>PLA2G12B<\/jats:italic>,<jats:italic>and PENK<\/jats:italic>), oxidant\/antioxidant balance (eg,<jats:italic>DUOXA2<\/jats:italic>and<jats:italic>GSTO1\u20101<\/jats:italic>), and stress (eg,<jats:italic>V1aR, GRLF1<\/jats:italic>,<jats:italic>Homer\u20102<\/jats:italic>, and<jats:italic>MAOB<\/jats:italic>). All these genes were up\u2010regulated, except down\u2010regulated<jats:italic>Homer\u20102<\/jats:italic>and<jats:italic>MAOB. DUOXA2<\/jats:italic>,<jats:italic>ALOX15B<\/jats:italic>,<jats:italic>PLA2G12B, MAOB<\/jats:italic>, and<jats:italic>GRLF1<\/jats:italic>expression was further validated by RT\u2010<jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content>. An increase in glutathione peroxidase (GPx) activity in heparinized whole blood of IAD\u2010affected Standardbred (<jats:italic>P\u00a0<\/jats:italic>=\u00a0.0025) and endurance horses (<jats:italic>P\u00a0<\/jats:italic>=\u00a0.0028) also suggests a deregulation of the oxidant\/antioxidant balance. There was good correlation (<jats:italic>r<\/jats:italic>\u00a0=\u00a0.7354) between BAL neutrophil percentage and whole blood GPx activity in all horses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study showed that circulating blood cell gene expression reflects inflammatory responses in tissues. Whether any of the genes have potential for diagnostic applications in the future remains to be investigated. Although not specific for<jats:styled-content style=\"fixed-case\">IAD<\/jats:styled-content>, whole blood<jats:styled-content style=\"fixed-case\">GP<\/jats:styled-content>x activity appears to be correlated with<jats:styled-content style=\"fixed-case\">BAL<\/jats:styled-content>neutrophil percentage. This finding should be further assessed by testing a larger number of horses.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vcp.12216","type":"journal-article","created":{"date-parts":[[2014,12,9]],"date-time":"2014-12-09T11:50:43Z","timestamp":1418125843000},"page":"37-46","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Expression microarray as a tool to identify differentially expressed genes in horses suffering from inflammatory airway disease"],"prefix":"10.1111","volume":"44","author":[{"given":"Eve","family":"Ramery","sequence":"first","affiliation":[{"name":"Department for Functional Sciences Faculty of Veterinary Medicine University of Liege Liege Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Audrey","family":"Fraipont","sequence":"additional","affiliation":[{"name":"Department for Functional Sciences Faculty of Veterinary Medicine University of Liege Liege 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