{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,7]],"date-time":"2024-06-07T00:06:20Z","timestamp":1717718780816},"reference-count":13,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,12,22]],"date-time":"2014-12-22T00:00:00Z","timestamp":1419206400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2015,3]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Vaccine reactions are described in cytology textbooks as having eosinophilic to magenta colored globules within and admixed with inflammatory cells. Recently, we have seen increased numbers of inflammatory lesions containing blue to blue\u2010gray globular material, with historical information suggesting an association with rabies vaccination.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>The purpose of the study was to confirm the blue\u2010gray and the eosinophilic material observed microscopically in some inflammatory lesions as being vaccine\u2010derived.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Three different vaccines were cytocentrifuged and Wright stained. Vaccine aliquots were also added to the culture media of canine\u2010derived macrophages for 24\u00a0hours and the cells subsequently harvested, cytocentrifuged, and Wright stained. The globular material present in both preparations was compared to that observed in vaccine\u2010induced inflammatory lesions. Morin staining was used to identify metal within vaccine material in both in vitro\u2010 and in vivo\u2010derived cytology samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Vaccine\u2010derived material has a characteristic color and appearance. Appearance of the material was consistent in cytologic samples, in cells incubated with the vaccine, and in cytocentrifuged preparations of the vaccine vial contents. The blue\u2010gray globules stained positively for Morin stain, while the eosinophilic material did not stain.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Vaccine\u2010induced inflammatory lesions may contain blue to blue\u2010gray or magenta stained globular material. Blue\u2010gray material was associated with administration of rabies vaccine Imrab 3<jats:styled-content style=\"fixed-case\">TF<\/jats:styled-content>and the observed material may be metal\u2010containing adjuvant. Magenta material was associated with other vaccines and negative for Morin stain, suggesting a metal\u2010free adjuvant.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vcp.12228","type":"journal-article","created":{"date-parts":[[2014,12,22]],"date-time":"2014-12-22T14:41:36Z","timestamp":1419259296000},"page":"152-156","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Identification of blue staining vaccine\u2010derived material in inflammatory lesions using cultured canine macrophages"],"prefix":"10.1111","volume":"44","author":[{"given":"Jennifer L.","family":"Scruggs","sequence":"first","affiliation":[{"name":"Department of Biomedical &amp; Diagnostic Sciences College of Veterinary Medicine The University of Tennessee Knoxville TN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Casey J.","family":"LeBlanc","sequence":"additional","affiliation":[{"name":"Department of Biomedical &amp; Diagnostic Sciences College of Veterinary Medicine The University of Tennessee Knoxville TN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,12,22]]},"reference":[{"key":"e_1_2_3_2_1","first-page":"28","volume-title":"Canine and Feline Cytology, A Color Atlas and Interpretation Guide","author":"Raskin ER","year":"2010"},{"key":"e_1_2_3_3_1","first-page":"91","volume-title":"Cowell and Tyler's Diagnostic Cytology and Hematology of the Dog and Cat","author":"Fisher DJ","year":"2013"},{"key":"e_1_2_3_4_1","first-page":"15","article-title":"Top 5 skin masses diagnosed with in\u2010house cytology","volume":"5","author":"Boes KM","year":"2013","journal-title":"Clin. 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