{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,8]],"date-time":"2024-06-08T11:40:21Z","timestamp":1717846821833},"reference-count":53,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,4,13]],"date-time":"2015-04-13T00:00:00Z","timestamp":1428883200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"University of Illinois College of Veterinary Medicine Campus Animal Memorial Fund"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2015,6]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Oxidative stress inhibits albumin's ability to complex with cobalt. Feline serum\u2013cobalt binding has not been described.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>The objective was to develop a cobalt binding test for use with feline serum, and correlate the results with other biochemical and cellular constituents in blood, and with clinical diseases of cats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A colorimetric test of cobalt binding, based on the oxidation\u2013reduction reaction of Co<jats:sup>+2<\/jats:sup>and dithiothreitol, was developed using feline serum. The test was used to measure cobalt binding in stored serum from 176 cats presented to the University of Illinois Veterinary Teaching Hospital for a variety of disease conditions. Time\u2010matched hematology and biochemical data, and clinical information, were obtained from the medical record of each cat and correlated with the serum\u2013cobalt binding results.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Serial dilution of feline serum with phosphate\u2010buffered saline resulted in a highly linear decrease in serum\u2013cobalt binding (<jats:italic>r<\/jats:italic><jats:sup>2<\/jats:sup>\u00a0=\u00a0.9984). Serum\u2013cobalt binding of the clinical samples also correlated with albumin concentrations in a stepwise linear regression model (<jats:italic>r<\/jats:italic><jats:sup>2<\/jats:sup>\u00a0=\u00a0.425), and both cobalt binding and albumin were significantly decreased in cases of inflammation. Albumin and cobalt binding also shared significant correlations with several erythron variables, and serum concentration of total calcium and bilirubin.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The correlation of cobalt binding measured by a colorimetric test with albumin concentration in the clinical samples and with serum dilution is consistent with feline albumin\u2013cobalt complex formation. Hypoalbuminemia is the likely cause of reduced serum\u2013cobalt binding in inflammation and the correlations observed between cobalt binding and other variables.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vcp.12253","type":"journal-article","created":{"date-parts":[[2015,4,13]],"date-time":"2015-04-13T15:08:45Z","timestamp":1428937725000},"page":"275-286","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Characterization of feline serum\u2013cobalt binding"],"prefix":"10.1111","volume":"44","author":[{"given":"Amy N.","family":"Schnelle","sequence":"first","affiliation":[{"name":"Department of Pathobiology College of Veterinary Medicine University of Illinois at Urbana\u2010Champaign Champaign IL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne M.","family":"Barger","sequence":"additional","affiliation":[{"name":"Department of Pathobiology College of Veterinary Medicine University of Illinois 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