{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:22:38Z","timestamp":1787016158086,"version":"build-2736575974"},"reference-count":43,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,6,23]],"date-time":"2015-06-23T00:00:00Z","timestamp":1435017600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100001250","name":"Morris Animal Foundation","doi-asserted-by":"crossref","award":["D10CA-034"],"award-info":[{"award-number":["D10CA-034"]}],"id":[{"id":"10.13039\/100001250","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      The N\u2010terminal fragment of the prohormone B\u2010type natriuretic peptide (\n                      <jats:styled-content style=\"fixed-case\">NT<\/jats:styled-content>\n                      \u2010pro\n                      <jats:styled-content style=\"fixed-case\">BNP<\/jats:styled-content>\n                      ) and cardiac troponin\u2010I are candidate biomarkers for cardiac disease in dogs. The degree of biologic variation in these biomarkers has not previously been reported in healthy dogs or dogs with mitral regurgitation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      The purpose of the study was to derive estimates of intrinsic biologic variability and reference change values for\n                      <jats:styled-content style=\"fixed-case\">NT<\/jats:styled-content>\n                      \u2010pro\n                      <jats:styled-content style=\"fixed-case\">BNP<\/jats:styled-content>\n                      and cardiac troponin\u2010I in healthy dogs and dogs with mitral regurgitation grade\n                      <jats:styled-content style=\"fixed-case\">IB<\/jats:styled-content>\n                      and\n                      <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content>\n                      according to the International Small Animal Cardiac Health Council (\n                      <jats:styled-content style=\"fixed-case\">ISACHC<\/jats:styled-content>\n                      ) grading system.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Plasma and sera were collected weekly for up to 7 weeks from 12 control dogs and 9 dogs with mitral regurgitation.\n                      <jats:styled-content style=\"fixed-case\">NT<\/jats:styled-content>\n                      \u2010pro\n                      <jats:styled-content style=\"fixed-case\">BNP<\/jats:styled-content>\n                      and troponin\u2010I (C\u2010TnI) concentrations were determined. Indices of biologic variation such as reciprocal index of individuality (r\u2010IoI) and reference change values (\n                      <jats:styled-content style=\"fixed-case\">RCV<\/jats:styled-content>\n                      ) were calculated in both the groups.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Individuality was high in control dogs and dogs with grade\n                      <jats:styled-content style=\"fixed-case\">IB<\/jats:styled-content>\n                      and\n                      <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content>\n                      mitral valve regurgitation for both C\u2010TnI (r\u2010IoI 1.6 and 2) and\n                      <jats:styled-content style=\"fixed-case\">NT<\/jats:styled-content>\n                      \u2010pro\n                      <jats:styled-content style=\"fixed-case\">BNP<\/jats:styled-content>\n                      (1.5 and 2.7), while the 2\u2010sided\n                      <jats:styled-content style=\"fixed-case\">RCV<\/jats:styled-content>\n                      for\n                      <jats:styled-content style=\"fixed-case\">NT<\/jats:styled-content>\n                      \u2010pro\n                      <jats:styled-content style=\"fixed-case\">BNP<\/jats:styled-content>\n                      was significantly lower in dogs with mitral regurgitation (52.5% vs 99.4%, P&lt;0.01.).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      High individuality of these cardiac biomarkers suggests that, following diagnosis, these assays are best interpreted by serial determination in individual canine patients rather than by comparison to a population\u2010based reference interval. The smaller\n                      <jats:styled-content style=\"fixed-case\">RCV<\/jats:styled-content>\n                      values for dogs with mitral regurgitation suggest that smaller relative changes in\n                      <jats:styled-content style=\"fixed-case\">NT<\/jats:styled-content>\n                      \u2010pro\n                      <jats:styled-content style=\"fixed-case\">BNP<\/jats:styled-content>\n                      are clinically meaningful in these patients.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/vcp.12268","type":"journal-article","created":{"date-parts":[[2015,6,23]],"date-time":"2015-06-23T20:39:32Z","timestamp":1435091972000},"page":"420-430","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Biologic variability in\n                    <scp>NT<\/scp>\n                    \u2010pro\n                    <scp>BNP<\/scp>\n                    and cardiac troponin\u2010I in healthy dogs and dogs with mitral valve degeneration"],"prefix":"10.1111","volume":"44","author":[{"given":"Craig","family":"Ruaux","sequence":"first","affiliation":[{"name":"Department of Clinical Sciences College of Veterinary Medicine Oregon State University Corvallis OR USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine","family":"Scollan","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences College of Veterinary Medicine Oregon State University Corvallis OR USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan S.","family":"Suchodolski","sequence":"additional","affiliation":[{"name":"Gastrointestinal Laboratory College of Veterinary Medicine and Biomedical Sciences Texas A&amp;M University College Station TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f6rg M.","family":"Steiner","sequence":"additional","affiliation":[{"name":"Gastrointestinal Laboratory College of Veterinary Medicine and Biomedical Sciences Texas A&amp;M University College Station TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. 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