{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T16:21:00Z","timestamp":1770222060758,"version":"3.49.0"},"reference-count":8,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,7,2]],"date-time":"2015-07-02T00:00:00Z","timestamp":1435795200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"SNSF, Swiss National Science Foundation","award":["PBZHP3_147298"],"award-info":[{"award-number":["PBZHP3_147298"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A blood sample from a 14\u2010year\u2010old dog was submitted to the veterinary diagnostic laboratory of the University of Milan for marked leukocytosis with atypical cells. A diagnosis of chronic T\u2010cell lymphocytic leukemia (<jats:styled-content style=\"fixed-case\">CLL<\/jats:styled-content>) was made based on blood smear evaluation and flow cytometric phenotyping. A<jats:styled-content style=\"fixed-case\">CBC<\/jats:styled-content>by Sysmex<jats:styled-content style=\"fixed-case\">XT<\/jats:styled-content>\u20102000iV revealed a moderate normocytic normochromic anemia. Red blood cells counted by optic flow cytometry (<jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>\u2010O) resulted in a higher value than using electrical impedance (<jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>\u2010I). The relative reticulocyte count based on<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>content and size was 35.3%, while the manual reticulocyte count was &lt;\u00a01%. The<jats:styled-content style=\"fixed-case\">WBC<\/jats:styled-content>count of 1,562,680\u00a0cells\/\u03bcL was accompanied by a flag. Manual counts for<jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>and<jats:styled-content style=\"fixed-case\">WBC<\/jats:styled-content>using the B\u00fcrker chamber confirmed the Sysmex impedance results. Finally the manual<jats:styled-content style=\"fixed-case\">PCV<\/jats:styled-content>was lower than<jats:styled-content style=\"fixed-case\">HCT<\/jats:styled-content>by Sysmex. While Sysmex<jats:styled-content style=\"fixed-case\">XT<\/jats:styled-content>can differentiate between<jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>and<jats:styled-content style=\"fixed-case\">WBC<\/jats:styled-content>by impedance, even in the face of extreme lymphocytosis due to<jats:styled-content style=\"fixed-case\">CLL<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>\u2010O can be affected by bias, resulting in falsely increased<jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>and reticulocyte numbers. Overestimation of<jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>\u2010O may be due to incorrect Sysmex classification of leukemic cells or their fragments as reticulocytes. This phenomenon is known as pseudoreticulocytosis and can lead to misinterpretation of regenerative anemia. On the other side<jats:styled-content style=\"fixed-case\">PCV<\/jats:styled-content>can be affected by bias in<jats:styled-content style=\"fixed-case\">CLL<\/jats:styled-content>due to the trapping of<jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>in the buffy coat, resulting in a pink hue in the separation area. As<jats:styled-content style=\"fixed-case\">HGB<\/jats:styled-content>concentration is not affected by flow cytometric or other cell\u2010related artifacts it may represent the most reliable variable to assess the degree of anemia in cases of<jats:styled-content style=\"fixed-case\">CLL<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/vcp.12270","type":"journal-article","created":{"date-parts":[[2015,7,3]],"date-time":"2015-07-03T16:03:27Z","timestamp":1435939407000},"page":"337-341","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Analytic errors in Sysmex\u2010generated hematology results in blood from a dog with chronic lymphocytic leukemia"],"prefix":"10.1111","volume":"44","author":[{"given":"Marilisa","family":"Novacco","sequence":"first","affiliation":[{"name":"Department of Veterinary Sciences and Public Health University of Milan Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valeria","family":"Martini","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences and Public Health University of Milan Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmen","family":"Grande","sequence":"additional","affiliation":[{"name":"Clinica Veterinaria Roma Sud Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefano","family":"Comazzi","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences and Public Health University of Milan Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,7,2]]},"reference":[{"key":"e_1_2_3_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2257.1993.tb00119.x"},{"key":"e_1_2_3_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00277-007-0314-6"},{"key":"e_1_2_3_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2257.1997.00001.x"},{"key":"e_1_2_3_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2257.1996.00151.x"},{"key":"e_1_2_3_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.2011.00322.x"},{"key":"e_1_2_3_7_1","doi-asserted-by":"crossref","first-page":"237","DOI":"10.2460\/javma.1996.208.02.237","article-title":"Acute lymphoblastic leukemia, hypercalcemia, and pseudohyperkalemia in a dog","volume":"208","author":"Henry CJ","year":"1996","journal-title":"J Am Vet Med Assoc"},{"key":"e_1_2_3_8_1","first-page":"1054","volume-title":"Schalm's Veterinary Hematology","author":"Weiss DJ","year":"2010"},{"key":"e_1_2_3_9_1","doi-asserted-by":"publisher","DOI":"10.1006\/bcmd.2002.0494"}],"container-title":["Veterinary Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fvcp.12270","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vcp.12270","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,9]],"date-time":"2024-06-09T20:50:42Z","timestamp":1717966242000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vcp.12270"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,7,2]]},"references-count":8,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2015,9]]}},"alternative-id":["10.1111\/vcp.12270"],"URL":"https:\/\/doi.org\/10.1111\/vcp.12270","archive":["Portico"],"relation":{},"ISSN":["0275-6382","1939-165X"],"issn-type":[{"value":"0275-6382","type":"print"},{"value":"1939-165X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,7,2]]},"assertion":[{"value":"2015-07-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}