{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T23:33:05Z","timestamp":1784763185079,"version":"3.55.0"},"reference-count":19,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,10,26]],"date-time":"2015-10-26T00:00:00Z","timestamp":1445817600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Abaxis, Inc."}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2015,12]]},"abstract":"Background<\/jats:title>Rapid and precise measurement of total and differential nucleated cell counts is a crucial diagnostic component of cavitary and synovial fluid analyses.<\/jats:p><\/jats:sec>Objectives<\/jats:title>The objectives of this study included (1) evaluation of reliability and precision of canine and equine fluid total nucleated cell count (TNCC<\/jats:styled-content>) determined by the benchtop Abaxis VetScan HM<\/jats:styled-content>5, in comparison with the automated reference instruments ADVIA<\/jats:styled-content> 120 and the scil Vet abc, respectively, and (2) comparison of automated with manual canine differential nucleated cell counts.<\/jats:p><\/jats:sec>Methods<\/jats:title>The TNCC<\/jats:styled-content> and differential counts in canine pleural and peritoneal, and equine synovial fluids were determined on the Abaxis VetScan HM<\/jats:styled-content>5 and compared with the ADVIA<\/jats:styled-content> 120 and Vet abc analyzer, respectively. Statistical analyses included correlation, least squares fit linear regression, Passing\u2013Bablok regression, and Bland\u2013Altman difference plots. In addition, precision of the total cell count generated by the VetScan HM<\/jats:styled-content>5 was determined.<\/jats:p><\/jats:sec>Results<\/jats:title>Agreement was excellent without significant constant or proportional bias for canine cavitary fluid TNCC<\/jats:styled-content>. Automated and manual differential counts had R<\/jats:italic>2<\/jats:sup>\u00a0<\u00a0.5 for individual cell types (least squares fit linear regression). Equine synovial fluid TNCC<\/jats:styled-content> agreed but with some bias due to the VetScan HM<\/jats:styled-content>5 overestimating TNCC<\/jats:styled-content> compared to the Vet abc. Intra\u2010assay precision of the VetScan HM<\/jats:styled-content>5 in 3 fluid samples was 2\u201331%.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>The Abaxis VetScan HM<\/jats:styled-content>5 provided rapid, reliable TNCC<\/jats:styled-content> for canine and equine fluid samples. The differential nucleated cell count should be verified microscopically as counts from the VetScan HM<\/jats:styled-content>5 and also from the ADVIA<\/jats:styled-content> 120 were often incorrect in canine fluid samples.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vcp.12298","type":"journal-article","created":{"date-parts":[[2015,10,26]],"date-time":"2015-10-26T15:31:36Z","timestamp":1445873496000},"page":"570-579","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Total nucleated cell and leukocyte differential counts in canine pleural and peritoneal fluid and equine synovial fluid samples: comparison of automated and manual methods"],"prefix":"10.1111","volume":"44","author":[{"given":"Jean M.","family":"Brudvig","sequence":"first","affiliation":[{"name":"Department of Pathobiology and Diagnostic Investigation and Diagnostic Center for Population and Animal Health College of Veterinary Medicine Michigan State University East Lansing MI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheryl L.","family":"Swenson","sequence":"additional","affiliation":[{"name":"Department of Pathobiology and Diagnostic Investigation and Diagnostic Center for Population and Animal Health College of Veterinary Medicine Michigan State University East Lansing MI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,10,26]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"235","volume-title":"Diagnostic Cytology and Hematology of the Dog and Cat","author":"Rizzi TE","year":"2008"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.2009.00203.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-323-01317-8.50013-4"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lab.2005.04.004"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0339(200004)22:4<256::AID-DC13>3.0.CO;2-G"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.2008.00063.x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPY7J7OLASZAPC"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.2009.00128.x"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.2008.00107.x"},{"key":"e_1_2_6_11_1","first-page":"214","article-title":"Use of the ADVIA 120 hematology analyzer in the differential cytologic analysis of biological fluids (cerebrospinal, peritoneal, pleural, pericardial, synovial, and others)","volume":"9","author":"Aulesa C","year":"2003","journal-title":"Lab Hematol"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.156.21.674"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.2008.00036.x"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2006.242"},{"key":"e_1_2_6_15_1","first-page":"282","article-title":"A comparison of manual and electronic counting for total nucleated cell counts on synovial fluid from canine stifle joints","volume":"50","author":"Atilola MAO","year":"1986","journal-title":"Can J Vet Res"},{"key":"e_1_2_6_16_1","unstructured":"DeJongK SwensonC VapL FlintC OwensS.Validation of the Vetscan HM5 hematology analyzer for canine feline and equine blood samples. 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