{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T11:56:59Z","timestamp":1788782219288,"version":"build-2803163510"},"reference-count":42,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,1,12]],"date-time":"2016-01-12T00:00:00Z","timestamp":1452556800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"TOSOH Bioscience Ltd"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2016,3]]},"abstract":"\n Background<\/jats:title>\n \n Equine pituitary pars intermedia dysfunction (\n PPID<\/jats:styled-content>\n ) may be diagnosed by measuring baseline plasma adrenocorticotrophic hormone (\n ACTH<\/jats:styled-content>\n ). The Immulite 1000 analyzer uses an automated chemiluminescence enzyme assay, previously validated for measuring equine\n ACTH<\/jats:styled-content>\n . Recently, an automated bench\u2010top immunoassay analyzer (\n AIA<\/jats:styled-content>\n \u2010360), designed for analytes in people, became available for veterinary use.\n <\/jats:p>\n <\/jats:sec>\n \n Objectives<\/jats:title>\n \n Objectives were to evaluate analytic performance of the\n AIA<\/jats:styled-content>\n immunoassay for measuring equine\n ACTH<\/jats:styled-content>\n , and compare the results with those obtained by the Immulite.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Adrenocorticotrophic hormone was measured in plasma samples from 52 clinical cases. For the\n AIA<\/jats:styled-content>\n , within\u2010 and between\u2010run coefficients of variation (\n CV<\/jats:styled-content>\n ) were assessed, linearity and recovery studies performed, and observed total error (\n TE<\/jats:styled-content>\n obs<\/jats:sub>\n ) calculated. Correlation and agreement between the 2 analyzers were also evaluated.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Within\u2010run and between\u2010run\n CV<\/jats:styled-content>\n of the\n AIA<\/jats:styled-content>\n ranged from 2.3% to 4% and 3.5% to 8%, respectively.\n ACTH<\/jats:styled-content>\n recoveries ranged from 89.5% to 115.9%.\n TE<\/jats:styled-content>\n obs<\/jats:sub>\n at 26.5 pg\/mL\n ACTH<\/jats:styled-content>\n was 4.1 pg\/mL. The\n ACTH<\/jats:styled-content>\n results (median: 25.9 pg\/mL; range: 4.3\u2013276.7 pg\/mL) with\n AIA<\/jats:styled-content>\n were significantly lower (\n P<\/jats:italic>\n < .0001) than with the Immulite (median: 29.9 pg\/mL; range: 10.3\u2013639.0 pg\/mL). Correlation between the 2 analyzers was\n r<\/jats:italic>\n = 0.882 (\n P<\/jats:italic>\n < .0001), with a significant bias for the\n AIA<\/jats:styled-content>\n of \u221216 pg\/mL. The 2 methods were not identical within inherent imprecision.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n The\n AIA<\/jats:styled-content>\n is precise for measuring\n ACTH<\/jats:styled-content>\n in horses. Although correlation between the instruments is good, the values obtained by the immunoassays cannot be used interchangeably and should be interpreted using reference intervals established for each analyzer to avoid false negatives. Diagnostic sensitivity and specificity of the\n AIA<\/jats:styled-content>\n \u2010360 should be evaluated before clinical use.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/vcp.12326","type":"journal-article","created":{"date-parts":[[2016,1,12]],"date-time":"2016-01-12T10:22:27Z","timestamp":1452594147000},"page":"154-163","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Initial analytic quality assessment and method comparison of an immunoassay for adrenocorticotropic hormone measurement in equine samples"],"prefix":"10.1111","volume":"45","author":[{"given":"Katherine L.","family":"Irvine","sequence":"first","affiliation":[{"name":"School of Veterinary Sciences University of Bristol Langford Bristol UK"},{"name":"Diagnostic Laboratories Langford Veterinary Services University of Bristol Bristol UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kay","family":"Burt","sequence":"additional","affiliation":[{"name":"School of Veterinary Sciences University of Bristol Langford Bristol UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew J.","family":"Hill","sequence":"additional","affiliation":[{"name":"Equine Centre Langford Veterinary Services University of Bristol Bristol UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie","family":"Shaw","sequence":"additional","affiliation":[{"name":"TOSOH Bioscience Ltd The Business Centre Redditch UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kostas","family":"Papasouliotis","sequence":"additional","affiliation":[{"name":"School of Veterinary Sciences University of Bristol Langford Bristol UK"},{"name":"Diagnostic Laboratories Langford Veterinary Services University of Bristol Bristol UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,1,12]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.2460\/javma.2000.217.1195"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.2007.tb00202.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0442.1994.tb00061.x"},{"key":"e_1_2_6_5_1","first-page":"381","article-title":"Evaluation of the dry chemical analysis system VetTest 8008","volume":"137","author":"Tschudi PR","year":"1995","journal-title":"Schweiz Arch Tierheilkd"},{"key":"e_1_2_6_6_1","first-page":"231","article-title":"Use of the dry chemistry \u201cReflotron\u201d blood analyzer under outdoor\u2010field conditions in veterinary medicine","volume":"34","author":"Lopes\u2010Pereira CM","year":"1996","journal-title":"Eur J Clin Chem Clin Biochem"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.5402\/2012\/475419"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.2460\/javma.2003.223.93"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0749-0739(02)00018-4"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2826.2005.01277.x"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-123-3-1598"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1354\/vp.45-1-26"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2012.00932.x"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0739-7240(94)00006-M"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.1996.tb02016.x"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.2746\/042516402776250333"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2010.04.014"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-3306.2011.00444.x"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jevs.2008.04.007"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/evj.12114"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/jsap.12181"},{"key":"e_1_2_6_22_1","unstructured":"LabCorp: Adrenocorticotropic Hormone (ACTH) Plasma. 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