{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T00:06:58Z","timestamp":1788307618802,"version":"build-2803163510"},"reference-count":44,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,5,26]],"date-time":"2016-05-26T00:00:00Z","timestamp":1464220800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"MSD Animal Health"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2016,6]]},"abstract":"Background<\/jats:title>Serum cystatin C (sCysC) is used as biomarker for glomerular filtration rate (GFR<\/jats:styled-content>). The effects of diabetes mellitus (DM<\/jats:styled-content>) on renal function in dogs are unclear. Some renal variables have been evaluated in dogs with hyperadrenocorticism (HAC<\/jats:styled-content>), but notsC<\/jats:styled-content>ysC.<\/jats:p><\/jats:sec>Objectives<\/jats:title>The purpose of this study was the validation of a particle\u2010enhanced nephelometric immunoassay (PENIA<\/jats:styled-content>) for measuring caninesC<\/jats:styled-content>ysC, and to assess renal function in dogs withDM<\/jats:styled-content>orHAC<\/jats:styled-content>.<\/jats:p><\/jats:sec>Methods<\/jats:title>APENIA<\/jats:styled-content>was analytically validated for caninesC<\/jats:styled-content>ysC by determining imprecision and linearity. In a longitudinal 6\u2010month study, renal function of 14DM<\/jats:styled-content>dogs was assessed, using serum creatinine,GFR<\/jats:styled-content>, urinary protein\u2010to\u2010creatinine (UPC<\/jats:styled-content>) ratio, urinary markers, systolic blood pressure (SBP<\/jats:styled-content>), andsC<\/jats:styled-content>ysC, and compared to 17 healthy dogs at baseline. Furthermore,sC<\/jats:styled-content>ysC was measured at initial presentation and during a 12\u2010month follow\u2010up in 22HAC<\/jats:styled-content>dogs.<\/jats:p><\/jats:sec>Results<\/jats:title>ThesC<\/jats:styled-content>ysC intra\u2010 and inter\u2010assay variation coefficients were < 8% and highly linear (r<\/jats:italic>= .997). About 33% and 67% ofDM<\/jats:styled-content>dogs had persistent proteinuria and systemic hypertension, respectively, but there were no significant differences inGFR<\/jats:styled-content>,UPC<\/jats:styled-content>, and urinary markers over time, and compared with healthy dogs at initial presentation. Serum CysC decreased significantly (P<\/jats:italic>< .05) over time within theDM<\/jats:styled-content>group. It did not change significantly over time within theHAC<\/jats:styled-content>group.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>A PENIA measuredsC<\/jats:styled-content>ysC linearly and precisely. There were no clinically relevant renal alterations over time in dogs withDM<\/jats:styled-content>, although persistent proteinuria was observed. In dogs withHAC<\/jats:styled-content>,sC<\/jats:styled-content>ysC measurement was not useful, although significantGFR<\/jats:styled-content>changes occurred over time.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vcp.12365","type":"journal-article","created":{"date-parts":[[2016,5,26]],"date-time":"2016-05-26T16:52:44Z","timestamp":1464281564000},"page":"320-329","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Long\u2010term follow\u2010up of renal function assessing serum cystatin C in dogs with diabetes mellitus or hyperadrenocorticism"],"prefix":"10.1111","volume":"45","author":[{"given":"Sofie J. J.","family":"Marynissen","sequence":"first","affiliation":[{"name":"Department of Small Animal Medicine and Clinical Biology Faculty of Veterinary Medicine Ghent University Merelbeke Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascale M. Y.","family":"Smets","sequence":"additional","affiliation":[{"name":"Department of Small Animal Medicine and Clinical Biology Faculty of Veterinary Medicine Ghent University Merelbeke Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liesbeth F. E.","family":"Ghys","sequence":"additional","affiliation":[{"name":"Department of Small Animal Medicine and Clinical Biology Faculty of Veterinary Medicine Ghent University Merelbeke Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"Paepe","sequence":"additional","affiliation":[{"name":"Department of Small Animal Medicine and Clinical Biology Faculty of Veterinary Medicine Ghent University Merelbeke Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joris","family":"Delanghe","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry, Microbiology and Immunology Faculty of Medicine and Health Sciences Ghent University Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Galac","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences of Companion Animals Faculty of Veterinary Medicine Utrecht University Utrecht The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evelyne","family":"Meyer","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Toxicology and Biochemistry Faculty of Veterinary Medicine Ghent University Merelbeke Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herv\u00e9 P.","family":"Lefebvre","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences and Clinical Research Unit INRA \u00c9cole Nationale V\u00e9t\u00e9rinaire de Toulouse (ENVT) Toulouse France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvie","family":"Daminet","sequence":"additional","affiliation":[{"name":"Department of Small Animal Medicine and Clinical Biology Faculty of Veterinary Medicine Ghent University Merelbeke Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,5,26]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2009.0426.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1053\/ajkd.2000.8237"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diabet.2011.05.002"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.5326\/0440131"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2002.tb01605.x"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2007.tb03046.x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1556\/AVet.58.2010.1.6"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11259-005-0058-5"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11259-007-0044-1"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0009-8981(02)00044-X"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.3109\/00365519509110261"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0101-28002011000300006"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2010.07.004"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0031702"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2011.05.027"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2012.00915.x"},{"key":"e_1_2_6_18_1","first-page":"119","article-title":"Initiation, progression and remission of diabetic nephropathy","volume":"52","author":"Hovind P","year":"2005","journal-title":"Dan Med Bull"},{"key":"e_1_2_6_19_1","first-page":"377","article-title":"Remission and regression of diabetic nephropathy","volume":"6","author":"Hovind P","year":"2004","journal-title":"Kidney Int"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.ajkd.2009.11.009"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.71.11.1387"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2003.00840.x"},{"key":"e_1_2_6_23_1","first-page":"1162","article-title":"Immunofluorescence studies of renal basement membranes in dogs with spontaneous diabetes","volume":"45","author":"Jeraj K","year":"1984","journal-title":"Am J Vet Res"},{"key":"e_1_2_6_24_1","doi-asserted-by":"crossref","first-page":"822","DOI":"10.2460\/javma.1998.213.06.822","article-title":"Systemic hypertension and proteinuria in dogs with diabetes mellitus","volume":"213","author":"Struble AL","year":"1998","journal-title":"J Am Vet Med Assoc"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11259-008-9133-z"},{"key":"e_1_2_6_26_1","first-page":"345","article-title":"Urine characteristics in dogs with diabetes mellitus. Is there a diabetic nephropathy in the dog?","volume":"21","author":"Kirsch M","year":"1993","journal-title":"Tierarztl Prax"},{"key":"e_1_2_6_27_1","unstructured":"ASVCP Quality Assurance Guidelines. Available at:http:\/\/www.asvcp.org\/pubs\/qas\/index.cfm. Accessed February 1 2015."},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.12144"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2009.0392.x"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2007.tb03005.x"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2005.tb02713.x"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2009.09.003"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2002.tb01603.x"},{"key":"e_1_2_6_34_1","first-page":"2000","volume-title":"Schalm's Veterinary Hematology","author":"Jensen AL","year":"2000"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/0161-5890(81)90136-X"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(82)90159-0"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2008.336"},{"key":"e_1_2_6_38_1","doi-asserted-by":"crossref","first-page":"1933","DOI":"10.1093\/clinchem\/38.10.1933","article-title":"Serum creatinine as an index of renal function: new insights into old concept","volume":"38","author":"Perrone RD","year":"1992","journal-title":"Clin Chem"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11255-012-0252-7"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1177\/1098612X12464628"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.12286"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00404138"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0300-595X(86)80074-3"},{"key":"e_1_2_6_44_1","first-page":"551","article-title":"The role of tubular injury in diabetic nephropathy","volume":"20","author":"Magri CJ","year":"2009","journal-title":"J Vet Intern Med"},{"key":"e_1_2_6_45_1","unstructured":"ZiniE BenaliS CoppolaL et al.Renal Morphology and Function in Cats with Diabetes Mellitus [Abstract]. Proceedings of the 22nd ECVIM\u2010CA Congress. Maastricht The Netherlands September 6\u20138 2012."}],"container-title":["Veterinary Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fvcp.12365","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vcp.12365","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T04:51:35Z","timestamp":1718599895000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vcp.12365"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,5,26]]},"references-count":44,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2016,6]]}},"alternative-id":["10.1111\/vcp.12365"],"URL":"https:\/\/doi.org\/10.1111\/vcp.12365","archive":["Portico"],"relation":{},"ISSN":["0275-6382","1939-165X"],"issn-type":[{"value":"0275-6382","type":"print"},{"value":"1939-165X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,5,26]]},"assertion":[{"value":"2016-05-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}