{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T11:27:04Z","timestamp":1770377224499,"version":"3.49.0"},"reference-count":12,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,7,5]],"date-time":"2016-07-05T00:00:00Z","timestamp":1467676800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Sir James Dunn Animal Welfare Center"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>A major cross\u2010match gel tube test is available for use in dogs yet has not been clinically evaluated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>This study compared cross\u2010match results obtained using the gel tube and the standard tube methods for canine samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Study 1 included 107 canine sample donor\u2013recipient pairings cross\u2010match tested with the RapidVet\u2010H method gel tube test and compared results with the standard tube method. Additionally, 120 pairings using pooled sera containing anti\u2010canine erythrocyte antibody at various concentrations were tested with leftover blood from a hospital population to assess sensitivity and specificity of the gel tube method in comparison with the standard method.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The gel tube method had a good relative specificity of 96.1% in detecting lack of agglutination (compatibility) compared to the standard tube method. Agreement between the 2 methods was moderate. Nine of 107 pairings showed agglutination\/incompatibility on either test, too few to allow reliable calculation of relative sensitivity. Fifty percent of the gel tube method results were difficult to interpret due to sample spreading in the reaction and\/or negative control tubes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The RapidVet\u2010H method agreed with the standard cross\u2010match method on compatible samples, but detected incompatibility in some sample pairs that were compatible with the standard method. Evaluation using larger numbers of incompatible pairings is needed to assess diagnostic utility. The gel tube method results were difficult to categorize due to sample spreading. Weak agglutination reactions or other factors such as centrifuge model may be responsible.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vcp.12374","type":"journal-article","created":{"date-parts":[[2016,7,5]],"date-time":"2016-07-05T15:16:23Z","timestamp":1467731783000},"page":"411-416","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Preliminary evaluation of a gel tube agglutination major cross\u2010match method in dogs"],"prefix":"10.1111","volume":"45","author":[{"given":"Dania","family":"Villarnovo","sequence":"first","affiliation":[{"name":"Department of Pathology and Microbiology Atlantic Veterinary College University of Prince Edward Island Charlottetown PE Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shelley A.","family":"Burton","sequence":"additional","affiliation":[{"name":"Department of Pathology and Microbiology Atlantic Veterinary College University of Prince Edward Island Charlottetown PE Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara S.","family":"Horney","sequence":"additional","affiliation":[{"name":"Department of Pathology and Microbiology Atlantic Veterinary College University of Prince Edward Island Charlottetown PE Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Allan L.","family":"MacKenzie","sequence":"additional","affiliation":[{"name":"Department of Pathology and Microbiology Atlantic Veterinary College University of Prince Edward Island Charlottetown PE Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rapha\u00ebl","family":"Vanderstichel","sequence":"additional","affiliation":[{"name":"Department of Health Management Atlantic Veterinary College University of Prince Edward Island Charlottetown PE Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,7,5]]},"reference":[{"key":"e_1_2_3_2_1","first-page":"711","volume-title":"Schalm's Veterinary Hematology","author":"Andrews GA","year":"2010"},{"key":"e_1_2_3_3_1","first-page":"1101","volume-title":"Schalm's Veterinary Hematology","author":"Wardrop KJ","year":"2010"},{"key":"e_1_2_3_4_1","first-page":"1105","volume-title":"Kirk's Current Veterinary Therapy XV","author":"Giger U","year":"2014"},{"key":"e_1_2_3_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvsm.2013.03.007"},{"key":"e_1_2_3_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.2006.tb00131.x"},{"key":"e_1_2_3_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2007.tb02961.x"},{"key":"e_1_2_3_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2007.tb02949.x"},{"key":"e_1_2_3_9_1","first-page":"97","volume-title":"Veterinary Epidemiologic Research","author":"Dohoo I","year":"2009"},{"key":"e_1_2_3_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvsm.2010.02.005"},{"key":"e_1_2_3_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3148.2006.00655.x"},{"key":"e_1_2_3_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.2010.00249.x"},{"key":"e_1_2_3_13_1","unstructured":"Alvedia immunochromatography cross\u2010match test.http:\/\/www.alvedia.com\/canine-lab-test-xm.html"}],"updated-by":[{"DOI":"10.1111\/vcp.12432","type":"erratum","label":"Erratum","source":"publisher","updated":{"date-parts":[[2016,11,21]],"date-time":"2016-11-21T00:00:00Z","timestamp":1479686400000}}],"container-title":["Veterinary Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fvcp.12374","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vcp.12374","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T12:38:29Z","timestamp":1695472709000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vcp.12374"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,7,5]]},"references-count":12,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2016,9]]}},"alternative-id":["10.1111\/vcp.12374"],"URL":"https:\/\/doi.org\/10.1111\/vcp.12374","archive":["Portico"],"relation":{},"ISSN":["0275-6382","1939-165X"],"issn-type":[{"value":"0275-6382","type":"print"},{"value":"1939-165X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,7,5]]},"assertion":[{"value":"2016-07-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}