{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T04:09:35Z","timestamp":1775707775116,"version":"3.50.1"},"reference-count":16,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,10,18]],"date-time":"2016-10-18T00:00:00Z","timestamp":1476748800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A 9\u2010year\u2010old, female Maltese dog was referred to the Veterinary School of Toulouse with a 2\u2010day history of anorexia and weakness. On clinical examination, the dog had hyperthermia (39.7\u00b0C), abdominal discomfort, and polypnea. Significant laboratory findings included pigmenturia, hyperbilirubinemia, hypercreatininemia, hyperfibrinogenemia, abnormal Snap canine pancreas\u2010specific lipase, and pancytopenia with a nonregenerative anemia. A peripheral blood smear revealed numerous intraerythrocytic large <jats:italic>Babesia<\/jats:italic> but no polychromasia. There was a discrepancy between the absolute automated reticulocyte count (Sysmex reticulocyte count: 60 \u00d7 10<jats:sup>9<\/jats:sup>\/L; <jats:styled-content style=\"fixed-case\">RI<\/jats:styled-content> 19.4\u2013150.1 \u00d7 10<jats:sup>9<\/jats:sup>\/L) and the manual reticulocyte count (3.6 \u00d7 10<jats:sup>9<\/jats:sup>\/L) as well as the absence of polychromasia. The optical red blood cell scattergram showed an abnormal isolated reticulocyte cluster at the location of low\u2010fluorescence ratio cells. These findings were interpreted as erythrocytes parasitized by large <jats:italic>Babesia<\/jats:italic>. The discrepancy between the Sysmex reticulocyte count and the manual reticulocyte count has been reported previously in people with falciparum malaria and numerous intra\u2010erythrocytic <jats:italic>Plasmodium falciparum<\/jats:italic> organisms. This spurious reticulocyte profile and reticulocyte count were observed with the Sysmex <jats:styled-content style=\"fixed-case\">XT<\/jats:styled-content>\u20102000iV and the ProCyte using the same fluorescent dye polymethine but not with the LaserCyte using new methylene blue which does not stain <jats:italic>Babesia<\/jats:italic> organisms on a blood smear performed for manual reticulocyte counting.<\/jats:p>","DOI":"10.1111\/vcp.12395","type":"journal-article","created":{"date-parts":[[2016,10,18]],"date-time":"2016-10-18T16:26:43Z","timestamp":1476808003000},"page":"594-597","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Spurious reticulocyte profiles in a dog with babesiosis"],"prefix":"10.1111","volume":"45","author":[{"given":"Laetitia","family":"Piane","sequence":"first","affiliation":[{"name":"Equipe de Biologie M\u00e9dicale\u2010Histologie CREFRE INSERM UPS ENVT Universit\u00e9 de Toulouse Toulouse France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie\u2010Laure","family":"Th\u00e9ron","sequence":"additional","affiliation":[{"name":"Unit\u00e9 d'Urgence et soins intensifs UPS INP ENVT Universit\u00e9 de Toulouse Toulouse France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcel","family":"Aumann","sequence":"additional","affiliation":[{"name":"Unit\u00e9 d'Urgence et soins intensifs UPS INP ENVT Universit\u00e9 de Toulouse Toulouse France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine","family":"Trumel","sequence":"additional","affiliation":[{"name":"Equipe de Biologie M\u00e9dicale\u2010Histologie CREFRE INSERM UPS ENVT Universit\u00e9 de Toulouse Toulouse France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,10,18]]},"reference":[{"key":"e_1_2_3_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199712253372615"},{"key":"e_1_2_3_3_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2257.2002.00426.x"},{"key":"e_1_2_3_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.2009.00125.x"},{"key":"e_1_2_3_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.2012.00432.x"},{"key":"e_1_2_3_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.2008.00084.x"},{"key":"e_1_2_3_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00580-006-0602-x"},{"key":"e_1_2_3_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2257.2006.00871.x"},{"key":"e_1_2_3_9_1","first-page":"373","article-title":"Heinz bodies interfere with automated reticulocyte count","volume":"84","author":"Espagnol I","year":"1999","journal-title":"Haematologica"},{"key":"e_1_2_3_10_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.46.9.878"},{"key":"e_1_2_3_11_1","first-page":"92","article-title":"Laboratory evaluation of the Coulter STKS reticulocyte method in a children's hospital","volume":"3","author":"Ghevaert C","year":"1997","journal-title":"Lab Hematol"},{"key":"e_1_2_3_12_1","first-page":"125","article-title":"Reticulocyte counting by flow cytometry. 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