{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T06:36:52Z","timestamp":1785393412537,"version":"3.55.0"},"reference-count":14,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,12,21]],"date-time":"2016-12-21T00:00:00Z","timestamp":1482278400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>An 11\u2010year\u2010old, 443\u2010kg Haflinger mare was presented to the North Carolina State University Veterinary Teaching Hospital with a 2\u2010week history of lethargy and a 3\u2010day duration of anorexia, pyrexia, tachycardia, and ventral edema. Severe pitting edema, peripheral lymphadenopathy, and a caudal abdominal mass were noted on physical examination. An extreme leukocytosis (154.3 \u00d7 10<jats:sup>3<\/jats:sup>\/\u03bcL) and microscopic hematologic findings suggestive of myelomonocytic leukemia were observed. Serum protein electrophoresis revealed a monoclonal gammopathy and urine protein electrophoresis revealed a monoclonal light chain proteinuria. Necropsy and histopathology confirmed widespread neoplastic infiltration in many organs with a heterogenous population of cells; there was no apparent evidence of bone marrow involvement. Immunohistochemistry confirmed presence of a majority of B cells with a limited antigen expression, admixed with a lower number of T cells. Molecular clonality analysis of IgH2, IgH3, and kappa\u2010deleting element (<jats:styled-content style=\"fixed-case\">KDE<\/jats:styled-content>, B cell) on whole blood and<jats:styled-content style=\"fixed-case\">KDE<\/jats:styled-content>on infiltrated tissues revealed clonal rearrangements, and the<jats:styled-content style=\"fixed-case\">KDE<\/jats:styled-content>intron clones that amplified in blood and in infiltrated tissue were identical. In contrast, the clonality analysis of T\u2010cell receptor \u03b3 revealed no clonality on blood cells and infiltrated tissues. In conjunction with the histopathologic changes, the lesion was interpreted to be composed of neoplastic B cells with a reactive T\u2010cell population. Polymerase chain reaction testing for equine herpes virus 5 was negative. The final diagnosis was diffuse large B\u2010cell lymphoma with a marked hematogenous component.<\/jats:p>","DOI":"10.1111\/vcp.12435","type":"journal-article","created":{"date-parts":[[2016,12,21]],"date-time":"2016-12-21T21:10:23Z","timestamp":1482354623000},"page":"64-71","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Extreme lymphocytosis with myelomonocytic morphology in a horse with diffuse large B\u2010cell lymphoma"],"prefix":"10.1111","volume":"46","author":[{"given":"Kristina","family":"Meichner","sequence":"first","affiliation":[{"name":"Department of Pathology University of Georgia College of Veterinary Medicine Athens GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Blaire H.","family":"Kraszeski","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences College of Veterinary Medicine North Carolina State University Raleigh NC USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica R.","family":"Durrant","sequence":"additional","affiliation":[{"name":"Department of Population Health and Pathobiology College of Veterinary Medicine North Carolina State University Raleigh NC USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carol B.","family":"Grindem","sequence":"additional","affiliation":[{"name":"Department of Population Health and Pathobiology College of Veterinary Medicine North Carolina State University Raleigh NC USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Babetta A.","family":"Breuhaus","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences College of Veterinary Medicine North Carolina State University Raleigh NC USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter F.","family":"Moore","sequence":"additional","affiliation":[{"name":"Department of Pathology, Microbiology, and Immunology 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