{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T09:49:21Z","timestamp":1786787361342,"version":"build-2736575974"},"reference-count":13,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,2,13]],"date-time":"2017-02-13T00:00:00Z","timestamp":1486944000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Cell blocks are widely used in human cytopathology. Several techniques have been proposed to convert fluid specimens into solid material, which after embedding in paraffin can be used for histochemistry, immunohistochemistry, or molecular testing. In contrast, only in the last few years, have cell blocks begun to be used in the veterinary field.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>The purpose of the study was to present the production and features of cell tube blocks (<jats:styled-content style=\"fixed-case\">CTB<\/jats:styled-content>) with veterinary liquid samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Liquid samples including cerebrospinal fluid, cutaneous cyst fluid, pericardial and pleural effusions, bronchoalveolar lavage, urine, and blood were centrifuged in a microhematocrit centrifuge. Capillary tubes were broken at the liquid\u2013solid interface and fixed in 10% formalin for 24 hours. After paraffin embedding, sections of<jats:styled-content style=\"fixed-case\">CTB<\/jats:styled-content>were used for different stains including immunohistochemistry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The morphology and cellular detail in<jats:styled-content style=\"fixed-case\">CTB<\/jats:styled-content>sections were comparable to conventional histologic sections and other existing cell block techniques. The use of special stains such as Gram, Giemsa, alcian blue, and periodic acid\u2013Schiff was straightforward, and immunohistochemistry results with antibodies to pan\u2010cytokeratin,<jats:styled-content style=\"fixed-case\">PAX<\/jats:styled-content>\u20105, and<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>3 were considered good.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The<jats:styled-content style=\"fixed-case\">CTB<\/jats:styled-content>method was easily implementable under practice conditions (up to the fixation of the microhemtocrit tube), analogous to surgical biopsy submission for histology. Cell tube blocks can increase diagnostic accuracy when the technique is used in tandem after the cytologic evaluation, and the technique allows storage of fluids. Other advantages of<jats:styled-content style=\"fixed-case\">CTB<\/jats:styled-content>were the simplicity, low cost, and separation of erythrocytes from the nucleated cells, which was helpful in hemodiluted samples.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vcp.12446","type":"journal-article","created":{"date-parts":[[2017,2,13]],"date-time":"2017-02-13T15:24:00Z","timestamp":1486999440000},"page":"195-201","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["Cell tube block: a new technique to produce cell blocks from fluid cytology samples"],"prefix":"10.1111","volume":"46","author":[{"given":"Ricardo","family":"Marcos","sequence":"first","affiliation":[{"name":"Cytology Diagnostic Services Institute of Biomedical Sciences Abel Salazar University of Porto ICBAS \u2013 UP Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Santos","sequence":"additional","affiliation":[{"name":"Cytology Diagnostic Services Institute of Biomedical Sciences Abel Salazar University of Porto ICBAS \u2013 UP Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla","family":"Marrinhas","sequence":"additional","affiliation":[{"name":"Cytology Diagnostic Services Institute of Biomedical Sciences Abel Salazar University of Porto ICBAS \u2013 UP Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Caniatti","sequence":"additional","affiliation":[{"name":"DIVET Dipartimento di Scienze Veterinarie e Sanit\u00e0 Pubblica Universit\u00e0 degli Studi di Milano Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,2,13]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM194708142370702"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/cyt.12174"},{"key":"e_1_2_6_4_1","first-page":"207","volume-title":"Cytopathologic Diagnosis of Serous Fluids","author":"Shidham VB","year":"2007"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0339(199807)19:1<70::AID-DC15>3.0.CO;2-5"},{"key":"e_1_2_6_6_1","first-page":"630","article-title":"The role of cell block in fine\u2010needle aspiration cytology","volume":"28","author":"Keyhani\u2010Rofahga S","year":"1984","journal-title":"Acta Cytol"},{"key":"e_1_2_6_7_1","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1111\/cyt.12222","article-title":"Cell block are not a substitute for cytology: why pathologists should understand cytopathology particularly in their chosen speciality","volume":"25","author":"Herbert A","year":"2004","journal-title":"Cytopathology"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/1040638712445763"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.2012.00456.x"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.12011"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.12239"},{"issue":"1","key":"e_1_2_6_12_1","first-page":"107","article-title":"Liquid\u2010based cytology and cell block immunohistochemistry in veterinary medicine: comparison with standard cytology for the evaluation of canine lymphoid samples","volume":"14","author":"Fernandes NCCA","year":"2015","journal-title":"Vet Comp Oncol"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.10039"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.4103\/0970-9371.93210"}],"container-title":["Veterinary Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fvcp.12446","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vcp.12446","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/vcp.12446","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vcp.12446","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,22]],"date-time":"2024-06-22T12:56:15Z","timestamp":1719060975000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vcp.12446"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,2,13]]},"references-count":13,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2017,3]]}},"alternative-id":["10.1111\/vcp.12446"],"URL":"https:\/\/doi.org\/10.1111\/vcp.12446","archive":["Portico"],"relation":{},"ISSN":["0275-6382","1939-165X"],"issn-type":[{"value":"0275-6382","type":"print"},{"value":"1939-165X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,2,13]]},"assertion":[{"value":"2017-02-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}