{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:22:25Z","timestamp":1787016145056,"version":"build-2736575974"},"reference-count":33,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,1,24]],"date-time":"2017-01-24T00:00:00Z","timestamp":1485216000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"University of Padua","award":["CPDA132881\/13"],"award-info":[{"award-number":["CPDA132881\/13"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2017,3]]},"abstract":"\n Background<\/jats:title>\n \n Impression cytology (\n IC<\/jats:styled-content>\n ) is a noninvasive technique in which filters are used to sample superficial layers of ocular epithelium.\n <\/jats:p>\n <\/jats:sec>\n \n Objectives<\/jats:title>\n \n The aim of this study was to compare cytology specimens obtained by\n IC<\/jats:styled-content>\n and cytobrush from healthy canine and feline eyes.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n Dogs and cats were prospectively sampled using polytetrafluorethylene filters on the right eye, and cytobrush on the left eye. Wright\u2013Giemsa\u2010stained specimens were evaluated by 2 observers. Cellularity, preservation, and morphology of cells and presence of goblet and inflammatory cells were scored with a 4\u2010grade scale. Inter\u2010observer agreement and effects of topical anesthesia were analyzed.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n In 20 canine\n IC<\/jats:styled-content>\n samples, 10 showed good cellularity (score 2\u20133) and 13 good preservation. Superficial epithelial cells (\n SEC<\/jats:styled-content>\n ) were present in 13\/20 of\n IC<\/jats:styled-content>\n , while basal\u2010intermediate cells (\n BIC<\/jats:styled-content>\n ) were seen in 14\/20. In 6\/20 and 7\/20, goblet and inflammatory cells were noted, respectively. In 20 cats, 15 of\n IC<\/jats:styled-content>\n showed good cellularity and 14 good preservation, and\n SEC<\/jats:styled-content>\n were present in 16\/20 of\n IC<\/jats:styled-content>\n and\n BIC<\/jats:styled-content>\n in 17\/20. In 13\/20 and 3\/20 cats, goblet cells and inflammatory cells were noted, respectively. Canine cytobrush specimens appeared well preserved (9\/20) and had good cellularity (8\/20). In feline cytobrush specimens, good preservation and cellularity were observed in 16\/20 and 14\/20, respectively. In both species, all cell types were present without a clear separation. There was moderate to fair agreement about cellular morphology in\n IC<\/jats:styled-content>\n between observers. Specimens obtained with and without anesthesia were comparable.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n Impression cytology allowed collection of samples with maintained cytoarchitecture, while cytoplasmatic and nuclear details were often difficult to evaluate.<\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/vcp.12450","type":"journal-article","created":{"date-parts":[[2017,1,24]],"date-time":"2017-01-24T11:42:51Z","timestamp":1485258171000},"page":"164-171","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Cytology of the healthy canine and feline ocular surface: comparison between cytobrush and impression technique"],"prefix":"10.1111","volume":"46","author":[{"given":"Anna","family":"Perazzi","sequence":"first","affiliation":[{"name":"Department of Animal Medicine, Production and Health University of Padua Padua Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federico","family":"Bonsembiante","sequence":"additional","affiliation":[{"name":"Department of Comparative Biomedicine and Food Science University of Padua Padua Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Elena","family":"Gelain","sequence":"additional","affiliation":[{"name":"Department of Comparative Biomedicine and Food Science University of Padua Padua Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Patruno","sequence":"additional","affiliation":[{"name":"Department of Comparative Biomedicine and Food Science University of Padua Padua Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enzo","family":"Di Iorio","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine University of Padua Padua Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angelo","family":"Migliorati","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine University of Padua Padua Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilaria","family":"Iacopetti","sequence":"additional","affiliation":[{"name":"Department of Animal Medicine, Production and Health University of Padua Padua Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,1,24]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1463-5224.2005.00414.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.09-3886"},{"key":"e_1_2_6_4_1","first-page":"619","volume-title":"Veterinary Ophtalmology","author":"Hendrix D","year":"1999"},{"key":"e_1_2_6_5_1","doi-asserted-by":"crossref","first-page":"722","DOI":"10.2460\/javma.1977.170.07.722","article-title":"Cytology of normal and inflamed conjunctivas in dogs and cats","volume":"170","author":"Lavach JD","year":"1977","journal-title":"J Am Vet Med Assoc"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.2012.00423.x"},{"key":"e_1_2_6_7_1","first-page":"493","article-title":"Evaluation of corneal and conjunctival epithelium with the use of impression cytology in mixed\u2010breed dogs diagnosed with keratoconjunctivits sicca","volume":"3","author":"Balicki I","year":"2011","journal-title":"Bull Vet Inst Pulawy"},{"key":"e_1_2_6_8_1","unstructured":"NapoleoneG De BiaseD LamagnaB et al.Conjunctival impression cytology in horse.LXVIII Sisvet Annual Meeting; 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