{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:23:19Z","timestamp":1787016199813,"version":"build-2736575974"},"reference-count":10,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,2,10]],"date-time":"2017-02-10T00:00:00Z","timestamp":1486684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["25\u20105318"],"award-info":[{"award-number":["25\u20105318"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2017,3]]},"abstract":"\n Background<\/jats:title>\n \n Immunocytochemistry (\n ICC<\/jats:styled-content>\n ) is an advanced diagnostic technique used in the field of veterinary cytology. We recently developed a rapid\n ICC<\/jats:styled-content>\n method for the detection of cytokeratin and vimentin in dogs, which helps to determine whether tumor cells are of epithelial or nonepithelial origin. However, the diagnostic value of this rapid\n ICC<\/jats:styled-content>\n method in neoplastic diseases of dogs has not been assessed yet.\n <\/jats:p>\n <\/jats:sec>\n \n Objectives<\/jats:title>\n \n The aim of the present study was to assess the diagnostic accuracy of rapid\n ICC<\/jats:styled-content>\n compared to standard immunohistochemistry (\n IHC<\/jats:styled-content>\n ).\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Air\u2010dried smear samples and formalin\u2010fixed paraffin sections were prepared from tumors excised from dogs (\n n<\/jats:italic>\n \u00a0=\u00a030). Immunosignals for cytokeratin and vimentin were detected in smear samples by rapid\n ICC<\/jats:styled-content>\n , and in paraffin sections by standard\n IHC<\/jats:styled-content>\n . Signals in smear samples detected by rapid\n ICC<\/jats:styled-content>\n were compared with positive staining in paraffin sections detected by standard\n IHC<\/jats:styled-content>\n and analyzed for statistical significance (kappa statistic).\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Rapid\n ICC<\/jats:styled-content>\n detected specific immunosignals in 25\/30 cases (83.3%), and nonspecific signals were detected in 5\/30 cases. Statistical analysis revealed fair agreement in epithelial tumors (\n n<\/jats:italic>\n \u00a0=\u00a016) with cytokeratin (\u03ba\u00a0=\u00a00.236) and vimentin (\u03ba\u00a0=\u00a00.294). In nonepithelial tumors (\n n<\/jats:italic>\n \u00a0=\u00a014), almost perfect agreement was demonstrated with cytokeratin (\u03ba\u00a0=\u00a00.857) and vimentin (\u03ba\u00a0=\u00a00.857).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n The rapid\n ICC<\/jats:styled-content>\n method can be a useful tool for the diagnostic cytology of neoplastic tissues in dogs.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/vcp.12462","type":"journal-article","created":{"date-parts":[[2017,2,10]],"date-time":"2017-02-10T11:12:45Z","timestamp":1486725165000},"page":"172-178","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Rapid immunocytochemistry for the detection of cytokeratin and vimentin: assessment of its diagnostic value in neoplastic diseases of dogs"],"prefix":"10.1111","volume":"46","author":[{"given":"Mariko","family":"Sawa","sequence":"first","affiliation":[{"name":"Laboratory of Veterinary Clinical Pathology Joint Faculty of Veterinary Medicine Kagoshima University Korimoto Kagoshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mayuko","family":"Inoue","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Clinical Pathology Joint Faculty of Veterinary Medicine Kagoshima University Korimoto Kagoshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akira","family":"Yabuki","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Clinical Pathology Joint Faculty of Veterinary Medicine Kagoshima University Korimoto Kagoshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moeko","family":"Kohyama","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Clinical Pathology Joint Faculty of Veterinary Medicine Kagoshima University Korimoto Kagoshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noriaki","family":"Miyoshi","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Pathology Joint Faculty of Veterinary Medicine Kagoshima University Korimoto Kagoshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asuka","family":"Setoguchi","sequence":"additional","affiliation":[{"name":"Laboratory of Small Animal Internal Medicine Joint Faculty of Veterinary Medicine Kagoshima University Korimoto Kagoshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Osamu","family":"Yamato","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Clinical Pathology Joint Faculty of Veterinary Medicine Kagoshima University Korimoto Kagoshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,2,10]]},"reference":[{"key":"e_1_2_3_2_1","first-page":"532","volume-title":"Diagnostic Cytology and Hematology of the Dog and Cat","author":"Bargar AM","year":"2014"},{"key":"e_1_2_3_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2303.2011.00887.x"},{"key":"e_1_2_3_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2005.10.001"},{"key":"e_1_2_3_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.2008.00017.x"},{"key":"e_1_2_3_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2012.03.013"},{"key":"e_1_2_3_7_1","doi-asserted-by":"publisher","DOI":"10.1354\/vp.38-6-636"},{"key":"e_1_2_3_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0046-8177(90)90231-S"},{"key":"e_1_2_3_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0046-8177(88)80205-3"},{"key":"e_1_2_3_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2014.09.014"},{"key":"e_1_2_3_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5829.2011.00313.x"}],"container-title":["Veterinary Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fvcp.12462","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vcp.12462","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/vcp.12462","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vcp.12462","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,8]],"date-time":"2023-09-08T09:49:34Z","timestamp":1694166574000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vcp.12462"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,2,10]]},"references-count":10,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2017,3]]}},"alternative-id":["10.1111\/vcp.12462"],"URL":"https:\/\/doi.org\/10.1111\/vcp.12462","archive":["Portico"],"relation":{},"ISSN":["0275-6382","1939-165X"],"issn-type":[{"value":"0275-6382","type":"print"},{"value":"1939-165X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,2,10]]},"assertion":[{"value":"2017-02-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}