{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,10]],"date-time":"2024-08-10T07:06:40Z","timestamp":1723273600961},"reference-count":39,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,4,17]],"date-time":"2017-04-17T00:00:00Z","timestamp":1492387200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Saving the Survivors NPC"},{"name":"South African Veterinary Foundation"},{"name":"Department of Companion Animal Clinical Studies of the University of Pretoria"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Plasma biochemistry analysis may aid the management of White rhinoceros with poaching or other injuries. Analyzer\u2010 and species\u2010specific <jats:styled-content style=\"fixed-case\">RI<\/jats:styled-content>s are necessary to evaluate health status and identify abnormalities.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>The purpose of the study was to perform a comparison study between the <jats:styled-content style=\"fixed-case\">IDEXX<\/jats:styled-content> VetTest and Roche Cobas Integra 400 Plus, and to generate analyzer\u2010specific <jats:styled-content style=\"fixed-case\">RI<\/jats:styled-content>s for White rhinoceros according to published guidelines.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Comparison was carried out using White rhinoceros plasma samples from healthy and diseased animals. Reference intervals for albumin, <jats:styled-content style=\"fixed-case\">ALT<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> AST<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> CK<\/jats:styled-content>, creatinine, <jats:styled-content style=\"fixed-case\">GGT<\/jats:styled-content>, globulins, glucose, phosphorus, total proteins, and urea were determined from a sample group of chemically immobilized healthy adult White rhinoceros from the Kruger National Park, South Africa.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>There was a significant analytic bias for all analytes between the 2 analyzers. Results for glucose and total protein were clinically equivalent based on total allowable error limits. Reference intervals were generated from 51 individuals (26 male, 25 female) for all analytes except <jats:styled-content style=\"fixed-case\">ALT<\/jats:styled-content> on the VetTest.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>The type, direction, and magnitude of bias between the VetTest and Cobas appeared to be species\u2010 and analyzer\u2010specific, when compared to other studies. These measures of bias may be used for comparability testing. Reference intervals for total proteins (VetTest 77\u2013108\u00a0g\/L, Cobas 77\u2013110\u00a0g\/L) and globulins (VetTest 47\u201379\u00a0g\/L, Cobas 51\u201387\u00a0g\/L) were high compared to other uneven\u2010toed ungulates.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Plasma samples from White rhinoceros can be evaluated on both the VetTest and Cobas. Results from this study will aid conservation efforts directed toward this species.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vcp.12490","type":"journal-article","created":{"date-parts":[[2017,4,17]],"date-time":"2017-04-17T16:16:40Z","timestamp":1492445800000},"page":"287-298","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Method comparison and generation of plasma biochemistry RIs for the White rhinoceros on a point\u2010of\u2010care and wet chemistry analyzer"],"prefix":"10.1111","volume":"46","author":[{"given":"Emma H.","family":"Hooijberg","sequence":"first","affiliation":[{"name":"Department of Companion Animal Clinical Studies Faculty of Veterinary Science University of Pretoria Pretoria South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerhard","family":"Steenkamp","sequence":"additional","affiliation":[{"name":"Department of Companion Animal Clinical Studies Faculty of Veterinary Science University of Pretoria Pretoria South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Buss","sequence":"additional","affiliation":[{"name":"Veterinary Wildlife Services South African National Parks Kruger National Park Skukuza South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amelia","family":"Goddard","sequence":"additional","affiliation":[{"name":"Department of Companion Animal Clinical Studies Faculty of Veterinary Science University of Pretoria Pretoria South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,4,17]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"crossref","unstructured":"EmslieR.Ceratotherium simum. 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