{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T17:12:21Z","timestamp":1790615541323,"version":"4.1.0"},"reference-count":13,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,6,21]],"date-time":"2017-06-21T00:00:00Z","timestamp":1498003200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2017,9]]},"abstract":"Background<\/jats:title>Measurement of pancreatic lipase in serum is being used for the diagnosis of pancreatitis.<\/jats:p><\/jats:sec>Objectives<\/jats:title>The aims of this study were to measure serum lipase activity by the v\u2010LIP<\/jats:styled-content>\u2010P slide and serum canine pancreatic lipase immunoreactivity (cPLI<\/jats:styled-content>) concentration by SpeccPL<\/jats:styled-content>in dogs with exocrine pancreatic insufficiency (EPI<\/jats:styled-content>) and in serum samples that were hemolyzed, lipemic, or icteric.<\/jats:p><\/jats:sec>Methods<\/jats:title>Serum samples from 50 dogs withEPI<\/jats:styled-content>, 8 samples spiked with canine hemoglobin, Intralipid, or ditaurobilirubin, and 8 samples from dogs with severe hypertriglyceridemia were evaluated using v\u2010LIP<\/jats:styled-content>\u2010P and SpeccPL<\/jats:styled-content>assays.<\/jats:p><\/jats:sec>Results<\/jats:title>Serum lipase activity (v\u2010LIP<\/jats:styled-content>\u2010P) was in the lower 20% of theRI<\/jats:styled-content>in 58% ofEPI<\/jats:styled-content>dogs, while serumcPLI<\/jats:styled-content>(as measured by SpeccPL<\/jats:styled-content>) was in the lower 20% of theRI<\/jats:styled-content>in 98% ofEPI<\/jats:styled-content>dogs. The mean (\u00b1SD<\/jats:styled-content>) observed\u2010to\u2010expected ratios for serum samples spiked with canine hemoglobin, Intralipid, or ditaurobilirubin were 63.0 \u00b1 25.1%, 489.2 \u00b1 469.7%, and 80.2 \u00b1 11.6% for the v\u2010LIP<\/jats:styled-content>\u2010P slide, respectively, and 99.3 \u00b1 6.8%, 96.9 \u00b1 9.4%, and 98.7 \u00b1 11.0% for SpeccPL<\/jats:styled-content>, respectively. However, naturally occurring hypertriglyceridemia did not appear to have a significant effect on serum lipase activity as measured by the v\u2010LIP<\/jats:styled-content>\u2010P slide.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>These results show that SpeccPL<\/jats:styled-content>is specific for pancreatic lipase, while the v\u2010LIP<\/jats:styled-content>\u2010P slide is not. In addition, hemolysis and lipemia had no effect on SpeccPL<\/jats:styled-content>, while severe icterus resulted in a slight decrease in SpeccPL<\/jats:styled-content>. Hemolysis and icterus had a significant effect on the v\u2010LIP<\/jats:styled-content>\u2010P slide. The effect of lipemia on the v\u2010LIP<\/jats:styled-content>\u2010P slide cannot be conclusively established based on this study.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vcp.12517","type":"journal-article","created":{"date-parts":[[2017,6,21]],"date-time":"2017-06-21T14:33:44Z","timestamp":1498055624000},"page":"508-515","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Specificity of, and influence of hemolysis, lipemia, and icterus on serum lipase activity as measured by the v\u2010LIP<\/scp>\u2010P slide"],"prefix":"10.1111","volume":"46","author":[{"given":"Joerg M.","family":"Steiner","sequence":"first","affiliation":[{"name":"Gastrointestinal Laboratory College of Veterinary Medicine and Biomedical Sciences Texas A&M University College Station TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robynne","family":"Gomez","sequence":"additional","affiliation":[{"name":"Gastrointestinal Laboratory College of Veterinary Medicine and Biomedical Sciences Texas A&M University College Station TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan S.","family":"Suchodolski","sequence":"additional","affiliation":[{"name":"Gastrointestinal Laboratory College of Veterinary Medicine and Biomedical Sciences Texas A&M University College Station TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan A.","family":"Lidbury","sequence":"additional","affiliation":[{"name":"Gastrointestinal Laboratory College of Veterinary Medicine and Biomedical Sciences Texas A&M University College Station TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,6,21]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.11-0166"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.12334"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.2005.tb00007.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.2002.63.722"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.67.1.84"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.72.3.302"},{"key":"e_1_2_6_8_1","first-page":"283","volume-title":"Small Animal Gastroenterology","author":"Steiner JM","year":"2008"},{"key":"e_1_2_6_9_1","doi-asserted-by":"crossref","first-page":"195","DOI":"10.2460\/javma.1988.192.02.195","article-title":"Sensitivity and specificity of radioimmunoassay of serum trypsin\u2010like immunoreactivity for the diagnosis of canine exocrine pancreatic insufficiency","volume":"192","author":"Williams DA","year":"1988","journal-title":"J Am Vet Med Assoc"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajo.2008.09.032"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.2010.00245.x"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.1992.tb00596.x"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.11613\/BM.2014.008"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.12253"}],"container-title":["Veterinary Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fvcp.12517","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vcp.12517","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,19]],"date-time":"2025-06-19T22:05:07Z","timestamp":1750370707000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vcp.12517"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,6,21]]},"references-count":13,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2017,9]]}},"alternative-id":["10.1111\/vcp.12517"],"URL":"https:\/\/doi.org\/10.1111\/vcp.12517","archive":["Portico"],"relation":{},"ISSN":["0275-6382","1939-165X"],"issn-type":[{"value":"0275-6382","type":"print"},{"value":"1939-165X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,6,21]]},"assertion":[{"value":"2017-06-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}