{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T13:11:54Z","timestamp":1744204314368},"reference-count":14,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,3,9]],"date-time":"2018-03-09T00:00:00Z","timestamp":1520553600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["25\u20105318"],"award-info":[{"award-number":["25\u20105318"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Immunocytochemistry (<jats:styled-content style=\"fixed-case\">ICC<\/jats:styled-content>) is utilized as an advanced technique in veterinary cytology. In tumor diagnosis, cytokeratin and vimentin are markers used to distinguish the origin of tumor cells. Standard enzyme\u2010based <jats:styled-content style=\"fixed-case\">ICC<\/jats:styled-content> has limitations in clinical use; and therefore, more convenient and reliable methods are needed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>The purpose of this study was to develop a rapid multiple immunofluorescent (<jats:styled-content style=\"fixed-case\">RMIF<\/jats:styled-content>) detection method for dual cytokeratin and vimentin staining on cytology slides in dogs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Air\u2010dried smear samples from solid tumors and sediments of pleural effusions were prepared from dogs (n = 14) that were admitted to the Veterinary Teaching Hospital, Kagoshima University, Japan. Mouse monoclonal anti\u2010human cytokeratin (<jats:styled-content style=\"fixed-case\">AE<\/jats:styled-content>1\/<jats:styled-content style=\"fixed-case\">AE<\/jats:styled-content>3) and rabbit monoclonal anti\u2010human vimentin (<jats:styled-content style=\"fixed-case\">SP<\/jats:styled-content>20) antibodies were used as primary antibodies, followed by staining with Alexa Fluor\u2010conjugated secondary antibodies. Staining using the <jats:styled-content style=\"fixed-case\">RMIF<\/jats:styled-content> method was compared with enzyme\u2010based <jats:styled-content style=\"fixed-case\">ICC<\/jats:styled-content> staining.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Rapid multiple immunofluorescent immunostaining was clear and specific in the evaluated smears, whereas the enzyme\u2010based <jats:styled-content style=\"fixed-case\">ICC<\/jats:styled-content> showed nonspecific signals. By using the <jats:styled-content style=\"fixed-case\">RMIF<\/jats:styled-content> staining method, epithelial cells, mesenchymal cells, and mesothelial cells could be classified on a single smear of a pleural effusion. In smears of lymph nodes with epithelial tumor metastases, the <jats:styled-content style=\"fixed-case\">RMIF<\/jats:styled-content> method successfully detected metastatic epithelial tumor cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The <jats:styled-content style=\"fixed-case\">RMIF<\/jats:styled-content> method might be a useful tool for diagnostic cytology in veterinary medicine.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vcp.12598","type":"journal-article","created":{"date-parts":[[2018,3,9]],"date-time":"2018-03-09T15:52:56Z","timestamp":1520610776000},"page":"326-332","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Rapid multiple immunofluorescent staining for the simultaneous detection of cytokeratin and vimentin in the cytology of canine tumors"],"prefix":"10.1111","volume":"47","author":[{"given":"Mariko","family":"Sawa","sequence":"first","affiliation":[{"name":"Laboratory of Veterinary Clinical Pathology Joint Faculty of Veterinary Medicine Kagoshima University Kagoshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akira","family":"Yabuki","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Clinical Pathology Joint Faculty of Veterinary Medicine Kagoshima University Kagoshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moeko","family":"Kohyama","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Clinical Pathology Joint Faculty of Veterinary Medicine Kagoshima University Kagoshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noriaki","family":"Miyoshi","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Pathology Joint Faculty of Veterinary Medicine Kagoshima University Kagoshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Osamu","family":"Yamato","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Clinical Pathology Joint Faculty of Veterinary Medicine Kagoshima University Kagoshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,3,9]]},"reference":[{"key":"e_1_2_2_2_1","first-page":"532","volume-title":"Diagnostic Cytology and Hematology of the Dog and Cat","author":"Barger AM","year":"2014"},{"key":"e_1_2_2_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.2009.00119.x"},{"key":"e_1_2_2_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.12292"},{"key":"e_1_2_2_5_1","doi-asserted-by":"publisher","DOI":"10.2478\/pjvs-2014-0020"},{"key":"e_1_2_2_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.2008.00017.x"},{"key":"e_1_2_2_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2005.10.001"},{"key":"e_1_2_2_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.12394"},{"key":"e_1_2_2_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.12462"},{"key":"e_1_2_2_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2012.03.013"},{"key":"e_1_2_2_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-04609-4"},{"key":"e_1_2_2_12_1","first-page":"244","volume-title":"Diagnostic Cytology and Hematology of the Dog and Cat","author":"Valenciano AC","year":"2014"},{"key":"e_1_2_2_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-1-4557-4083-3.00006-1"},{"key":"e_1_2_2_14_1","doi-asserted-by":"publisher","DOI":"10.1177\/1040638711416625"},{"key":"e_1_2_2_15_1","first-page":"431","volume-title":"Diagnostic Cytology and Hematology of the Dog and Cat","author":"Vobornik S","year":"2014"}],"container-title":["Veterinary Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fvcp.12598","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vcp.12598","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T00:55:11Z","timestamp":1695516911000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vcp.12598"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,3,9]]},"references-count":14,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2018,6]]}},"alternative-id":["10.1111\/vcp.12598"],"URL":"https:\/\/doi.org\/10.1111\/vcp.12598","archive":["Portico"],"relation":{},"ISSN":["0275-6382","1939-165X"],"issn-type":[{"value":"0275-6382","type":"print"},{"value":"1939-165X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,3,9]]},"assertion":[{"value":"2018-03-09","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}