{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T06:49:16Z","timestamp":1770274156903,"version":"3.49.0"},"reference-count":18,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,12,11]],"date-time":"2018-12-11T00:00:00Z","timestamp":1544486400000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2018,12]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Centrifugation is the primary method used to perform urine sediment analyses, but evaluation of other methods is required to validate centrifugation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Non\u2010urine materials were used to examine the repeatability (precision) and effectiveness (recovery) of four sediment methodologies on red blood cell (RBC) and white blood cell (WBC) counts.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Four urine sediment methods were compared using commercially available quality control material (QCM) and fresh canine RBCs in a diluent. Treatments included (a) 5\u00a0mL centrifugation at 390<jats:italic>g<\/jats:italic> for 5\u00a0minutes; (b) 1.5\u00a0mL centrifugation at 3900<jats:italic>g<\/jats:italic> for 45\u00a0seconds; (c) 60\u00a0\u00b5L of neat (unspun urine) in a microtiter well; and (d) 30\u00a0\u00b5L of neat on a slide with a coverslip. A within\u2010run precision using QCM was followed by a one\u2010run comparison test performed with a suspension of canine erythrocytes. RBC morphology was also examined.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All results are listed in order of Methods A\u2010D. Percent coefficients of variation (%CVs) for WBCs were 23.2%, 33.7%, 15.0%, and 27.2%. Red blood cells %CVs were 34.3%, 29.2%, 16.2%, and 24.4%. Average WBC counts in ten fields of view (FOV) \u00b1 1 SD were 26.4\u00a0\u00b1\u00a06.1, 14.2\u00a0\u00b1\u00a04.8, 32.8\u00a0\u00b1\u00a04.9, and 1.6\u00a0\u00b1\u00a00.4. Average RBC counts in 10 fields of view (FOV) \u00b1 1 SD were 45.3\u00a0\u00b1\u00a015.5, 23.9\u00a0\u00b1\u00a07.0, 38.4\u00a0\u00b1\u00a06.2, and 2.6\u00a0\u00b1\u00a00.6. The one\u2010run comparison test reports average RBC counts per FOV at 55.2, 23.4, 92.8, and 13.8. The percentages of abnormal RBCs were 92.2%, 74.8%, 7.0%, and 55.1%.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Method C had the best reproducibility, a lower frequency of cell morphology abnormalities, and similar cellular counts to those of Methods A and B.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vcp.12674","type":"journal-article","created":{"date-parts":[[2018,12,11]],"date-time":"2018-12-11T16:47:12Z","timestamp":1544546832000},"page":"608-616","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Urine sediment examination: Potential impact of red and white blood cell counts using different sediment methods"],"prefix":"10.1111","volume":"47","author":[{"given":"Julia","family":"Chase","sequence":"first","affiliation":[{"name":"IDEXX Laboratories, Inc. Westbrook Maine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeremy","family":"Hammond","sequence":"additional","affiliation":[{"name":"IDEXX Laboratories, Inc. Westbrook Maine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Graham","family":"Bilbrough","sequence":"additional","affiliation":[{"name":"IDEXX Laboratories, Inc. Westbrook Maine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dennis B.","family":"DeNicola","sequence":"additional","affiliation":[{"name":"IDEXX Laboratories, Inc. 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