{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T22:25:34Z","timestamp":1767651934144,"version":"3.37.3"},"reference-count":12,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,1,9]],"date-time":"2019-01-09T00:00:00Z","timestamp":1546992000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100004913","name":"Texas AgriLife Research","doi-asserted-by":"publisher","award":["TEXV 6579 (ProkectI-9524)"],"award-info":[{"award-number":["TEXV 6579 (ProkectI-9524)"]}],"id":[{"id":"10.13039\/100004913","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>An 8\u2010year\u2010old, neutered male, Golden Retriever presented for bilateral carpal joint effusion. A complete blood count revealed mild leukopenia and marked thrombocytopenia. Samples were sent to the Texas A&amp;M Veterinary Medical Diagnostic Laboratory for blood smear review and serologic testing for tick\u2010borne diseases. Numerous morulae were observed within neutrophils, and antibodies against <jats:italic>Ehrlichia canis<\/jats:italic> were detected at a 1:512 dilution via the indirect fluorescent antibody (IFA) test. As neutrophilic morulae are morphologically indistinguishable between <jats:italic>Ehrlichia ewingii<\/jats:italic> and <jats:italic>Anaplasma phagocytophilum,<\/jats:italic> and genus\u2010wide cross\u2010reactivity is possible with serologic testing, additional molecular testing was performed. Quantitative real\u2010time polymerase chain reaction (qPCR) followed by conventional PCR and Sanger sequencing were performed on serum identified with <jats:italic>E\u00a0ewingii <\/jats:italic>as the sole disease\u2010causing agent. Three months after diagnosis and treatment, no morulae were found, molecular testing for <jats:italic>E\u00a0ewingii<\/jats:italic> detected no DNA, and convalescent IFA testing demonstrated a continued detection of antibodies for <jats:italic>E\u00a0canis<\/jats:italic> at a 1:512 dilution. To the authors\u2019 knowledge, this is the first reported case of <jats:italic>E\u00a0ewingii<\/jats:italic> confirmed with molecular diagnostics in a Texas dog. The zoonotic transmission potential of <jats:italic>E\u00a0ewingii<\/jats:italic> should be noted as Texas supports competent tick vectors, and dogs represent effective sentinels for human ehrlichiosis. This report also highlights the utility of molecular diagnostics when serologic and microscopic evaluations are not sufficient in providing the species\u2010level identity of a causative agent.<\/jats:p>","DOI":"10.1111\/vcp.12690","type":"journal-article","created":{"date-parts":[[2019,1,9]],"date-time":"2019-01-09T16:58:45Z","timestamp":1547053125000},"page":"96-99","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Molecular identification of <i>Ehrlichia ewingii<\/i> in a polyarthritic Texas dog"],"prefix":"10.1111","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5543-7133","authenticated-orcid":false,"given":"Joseph J.","family":"Modarelli","sequence":"first","affiliation":[{"name":"Texas A&amp;M Veterinary Medical Diagnostic Laboratory College Station Texas"},{"name":"Department of Veterinary Pathobiology College of Veterinary Medicine and Biomedical Sciences College Station Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mindy M.","family":"Borst","sequence":"additional","affiliation":[{"name":"Texas A&amp;M Veterinary Medical Diagnostic Laboratory College Station Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julie","family":"Piccione","sequence":"additional","affiliation":[{"name":"Texas A&amp;M Veterinary Medical Diagnostic Laboratory College Station Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5921-2794","authenticated-orcid":false,"given":"Maria D.","family":"Esteve\u2010Gasent","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology College of Veterinary Medicine and Biomedical Sciences College Station Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,1,9]]},"reference":[{"key":"e_1_2_4_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/104063878900100212"},{"key":"e_1_2_4_3_1","doi-asserted-by":"crossref","first-page":"1852","DOI":"10.1128\/jcm.35.7.1852-1855.1997","article-title":"Comparison of nested PCR with immunofluorescent\u2010antibody assay for detection of Ehrlichia canis infection in dogs treated with doxycycline","volume":"35","author":"Wen B","year":"1997","journal-title":"J Clin Microbiol"},{"key":"e_1_2_4_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2002.tb02374.x"},{"key":"e_1_2_4_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.2009.00131.x"},{"key":"e_1_2_4_6_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.15-0540"},{"key":"e_1_2_4_7_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1007.03-0792"},{"key":"e_1_2_4_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ttbdis.2015.03.014"},{"key":"e_1_2_4_9_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2205.152009"},{"key":"e_1_2_4_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-5-29"},{"key":"e_1_2_4_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2010.05.006"},{"key":"e_1_2_4_12_1","article-title":"A serological survey of tick\u2010borne pathogens in dogs in North America and the Caribbean as assessed by Anaplasma phagocytophilum, A. platys, Ehrlichia canis, E. chaffeensis, E. ewingii, and Borrelia burgdorferi species\u2010specific peptides","volume":"4","author":"Qurollo BA","year":"2014","journal-title":"Infect Ecol Epidemiol"},{"key":"e_1_2_4_13_1","unstructured":"IDEXX.The SNAP4Dx Plus Test provides sensitive and specific detection of tick\u2010borne diseases 2016;https:\/\/idexxcom-live-b02da1e51e754c9cb292133b-9c56c33.aldryn-media.com\/filer_public\/34\/32\/34328f81-21ee-47aa-bd34-29084292e22d\/snap-4dx-plus-sensitivity-specificity.pdf. 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