{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T05:48:21Z","timestamp":1786945701051,"version":"build-2736575974"},"reference-count":10,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,3,12]],"date-time":"2019-03-12T00:00:00Z","timestamp":1552348800000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2016\/16023-8"],"award-info":[{"award-number":["2016\/16023-8"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2016\/11583-5"],"award-info":[{"award-number":["2016\/11583-5"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Sporotrichosis is an emerging zoonotic mycosis that presents as a cutaneous lymphatic or disseminated disease, caused by fungi from the <jats:italic>Sporothrix schenkii<\/jats:italic> (<jats:italic>S\u00a0schenkii<\/jats:italic>) clinical clade. Its importance is growing, primarily due to an outbreak that occurred in Brazil, affecting mainly cats and people.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>In Brazil, an <jats:italic>S\u00a0schenkii<\/jats:italic> diagnosis is often made using cultures, which allows genus identification and sufficient growth to perform molecular biology testing. Despite its advantages, fungal cultures are slow to develop and can delay public health measures, highlighting the importance of developing additional diagnostics techniques.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Cell block cytology (CBLC) is an older method that regained importance after liquid\u2010based cytology (LBC) was introduced, and it has been previously and successfully applied to veterinary diagnostics. We aimed to standardize and compare CBLC from cervical brush exfoliation of open wounds and fine\u2010needle aspirates with culture and immunohistochemistry of skin biopsies for sporotrichosis in cats, as a novel method.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>For this purpose, we selected 40 cats with skin lesions suspected of having sporotrichosis in Guarulhos city, S\u00e3o Paulo state, Brazil. We achieved 97.5% and 95% positivity using CBLC and culture, respectively, and 100% of feline skin biopsies were positive for <jats:italic>Sporothrix<\/jats:italic> spp on histopathology\/immunohistochemistry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Cell block cytology is an efficient and rapid tool to diagnose sporotrichosis in cats, particularly during epidemics.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vcp.12708","type":"journal-article","created":{"date-parts":[[2019,3,12]],"date-time":"2019-03-12T19:56:51Z","timestamp":1552420611000},"page":"143-147","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Feline <i>Sporothrix<\/i> spp. detection using cell blocks from brushings and fine\u2010needle aspirates: Performance and comparisons with culture and histopathology"],"prefix":"10.1111","volume":"48","author":[{"given":"Fernanda F.","family":"Gonsales","sequence":"first","affiliation":[{"name":"Department of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine University of S\u00e3o Paulo S\u00e3o Paulo SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nat\u00e1lia C. C. A.","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Anatomic Pathology Department, Pathology Center Adolfo Lutz Institute S\u00e3o Paulo SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wilson","family":"Mansho","sequence":"additional","affiliation":[{"name":"Technical division, Zoonosis Control Center of Guarulhos SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hildebrando","family":"Montenegro","sequence":"additional","affiliation":[{"name":"Zoonosis Control Center of S\u00e3o Paulo (COVISA\/SMS\/PMSP) S\u00e3o Paulo SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana M.","family":"Guerra","sequence":"additional","affiliation":[{"name":"Quantitative Pathology Department, Pathology Center Adolfo Lutz Institute S\u00e3o Paulo SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonardo Jos\u00e9 T.","family":"de Ara\u00fajo","sequence":"additional","affiliation":[{"name":"Quantitative Pathology Department, Pathology Center Adolfo Lutz Institute S\u00e3o Paulo SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvana M. P.","family":"da Silva","sequence":"additional","affiliation":[{"name":"Anatomic Pathology Department, Pathology Center Adolfo Lutz Institute S\u00e3o Paulo SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nilson R.","family":"Benites","sequence":"additional","affiliation":[{"name":"Department of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine University of S\u00e3o Paulo S\u00e3o Paulo SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,3,12]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2014.01.004"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-014-0269-5"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/myc.12414"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myv038"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/mmy\/myu061"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.2012.00456.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.23707"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1309\/G035-P2MM-D1TM-T5QE"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/vco.12137"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006077"}],"container-title":["Veterinary Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fvcp.12708","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vcp.12708","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/vcp.12708","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vcp.12708","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T19:16:40Z","timestamp":1694373400000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vcp.12708"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3]]},"references-count":10,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,3]]}},"alternative-id":["10.1111\/vcp.12708"],"URL":"https:\/\/doi.org\/10.1111\/vcp.12708","archive":["Portico"],"relation":{},"ISSN":["0275-6382","1939-165X"],"issn-type":[{"value":"0275-6382","type":"print"},{"value":"1939-165X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,3]]},"assertion":[{"value":"2018-05-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-08-31","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-12","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}