{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T17:03:07Z","timestamp":1770224587772,"version":"3.49.0"},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,3,21]],"date-time":"2019-03-21T00:00:00Z","timestamp":1553126400000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A 2\u2010year\u2010old female spayed Boxer dog was presented for a 1\u2010month history of progressive hemorrhagic diarrhea with tenesmus and weight loss despite trial courses of antibiotics and diet change. Abdominal ultrasound revealed severe, focal thickening, and loss of normal architecture of the colonic wall with abdominal lymphadenomegaly. Dry\u2010mount fecal cytology, performed on several consecutive days, consistently revealed numerous, round, 16\u201020\u00a0\u03bcm structures with basophilic, granular content, and a thin cell wall. Transmission electron microscopy identified these structures as fungi. Culture, polymerase chain reaction (PCR), and sequencing of the internal transcribed spacer, D1\/D2 regions, and DNA\u2010directed RNA polymerase II core subunit (RPB2) confirmed the presence of <jats:italic>Basidiobolus microsporus<\/jats:italic> in the feces. Biopsies collected via ileocolonoscopy revealed marked, multifocal, chronic, neutrophilic, and eosinophilic ileitis and colitis with ulceration, granulation tissue, and intralesional hyphae (identified with Gomori methenamine silver stain). A <jats:italic>Pythium<\/jats:italic> enzyme\u2010linked immunosorbent assay and <jats:italic>Pythium<\/jats:italic>\u2010specific PCR performed on the formalin\u2010fixed paraffin\u2010embedded biopsy specimens were positive while <jats:italic>Basidiobolus<\/jats:italic>\u2010specific PCR was negative, thus confirming a diagnosis of pythiosis. This report describes a fatal case of colonic and intestinal pythiosis with the presence of fecal <jats:italic>Basidiobolus<\/jats:italic> sp. spores, suggestive of concurrent gastrointestinal basidiobolomycosis.<\/jats:p>","DOI":"10.1111\/vcp.12720","type":"journal-article","created":{"date-parts":[[2019,3,21]],"date-time":"2019-03-21T18:11:52Z","timestamp":1553191912000},"page":"83-88","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Gastrointestinal pythiosis with concurrent presumptive gastrointestinal basidiobolomycosis in a Boxer dog"],"prefix":"10.1111","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3605-0822","authenticated-orcid":false,"given":"Joseph C.","family":"Parambeth","sequence":"first","affiliation":[{"name":"Departments of Small Animal Clinical Sciences College of Veterinary Medicine &amp; Biomedical Sciences Texas A&amp;M University College Station Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara D.","family":"Lawhon","sequence":"additional","affiliation":[{"name":"Veterinary Pathobiology College of Veterinary Medicine &amp; Biomedical Sciences Texas A&amp;M University College Station Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joanne","family":"Mansell","sequence":"additional","affiliation":[{"name":"Veterinary Pathobiology College of Veterinary Medicine &amp; Biomedical Sciences Texas A&amp;M University College Station Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Wu","sequence":"additional","affiliation":[{"name":"Veterinary Pathobiology College of Veterinary Medicine &amp; Biomedical Sciences Texas A&amp;M University College Station Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabrina D.","family":"Clark","sequence":"additional","affiliation":[{"name":"Veterinary Pathobiology College of Veterinary Medicine &amp; Biomedical Sciences Texas A&amp;M University College Station Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deanna","family":"Sutton","sequence":"additional","affiliation":[{"name":"Fungus Testing Laboratory UT Health Science Center San Antonio San Antonio Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Connie","family":"Gibas","sequence":"additional","affiliation":[{"name":"Fungus Testing Laboratory UT Health Science Center San Antonio San Antonio Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathan P.","family":"Wiederhold","sequence":"additional","affiliation":[{"name":"Fungus Testing Laboratory UT Health Science Center San Antonio San Antonio Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2680-6526","authenticated-orcid":false,"given":"Alexandra N.","family":"Myers","sequence":"additional","affiliation":[{"name":"Veterinary Pathobiology College of Veterinary Medicine &amp; 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