{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T16:17:39Z","timestamp":1759335459960},"reference-count":20,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,6,27]],"date-time":"2019-06-27T00:00:00Z","timestamp":1561593600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In this case report, a Swedish flat\u2010coated retriever was diagnosed with an extensive <jats:italic>Hepatozoon canis<\/jats:italic> infection. The dog had a prominent monocytosis (14.0\u00a0\u00d7\u00a010<jats:sup>9<\/jats:sup>\/L) with <jats:italic>H\u00a0canis<\/jats:italic> gamonts detected in most monocytes, but none were found in the neutrophils. On the hematology system ADVIA 2120 peroxidase (PEROX) cytogram, most leukocytes were seen as a distinct cell population above the lymphocytes, which indicated that most of the cells were larger than lymphocytes and had weak myeloperoxidase staining. This distinct cell cluster appeared to be of a single cell type but was incorrectly divided by the ADVIA 2120 into lymphocytes, monocytes, and large unstained cells (LUC). The total leukocyte counts on the ADVIA 2120 WBC basophil (BASO) channel were much higher than that on the WBC PEROX count. The WBC BASO cytogram appeared abnormal with two parallel cell populations, so the BASO WBC count was considered erroneous. Polymerase chain reaction and DNA sequencing verified <jats:italic>H\u00a0canis<\/jats:italic> infection. The dog was treated with subcutaneous imidocarb dipropionate (6\u00a0mg\/kg) injections every other week. Post\u2010treatment hematology analyses indicated that the percentage of parasitized leukocytes decreased from 40% to 5% about 4\u00a0weeks after the start of treatment and were not found in any monocytes 6\u00a0weeks after the beginning of the treatment. In conclusion, <jats:italic>H\u00a0canis<\/jats:italic> infection in this dog was associated with a strong monocytosis, and gamonts were present in many monocytes, which caused aberrant automated leukocyte counts to occur.<\/jats:p>","DOI":"10.1111\/vcp.12752","type":"journal-article","created":{"date-parts":[[2019,6,28]],"date-time":"2019-06-28T03:40:32Z","timestamp":1561693232000},"page":"435-440","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["<i>Hepatozoon canis<\/i> infection causing a strong monocytosis with intra\u2010monocytic gamonts and leading to erroneous leukocyte determinations"],"prefix":"10.1111","volume":"48","author":[{"given":"Inger","family":"Lillieh\u00f6\u00f6k","sequence":"first","affiliation":[{"name":"Department of Clinical Sciences Swedish University of Agricultural Sciences Uppsala Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harold W.","family":"Tvedten","sequence":"additional","affiliation":[{"name":"Clinical Pathology Laboratory University Animal Hospital, Swedish University of the Agricultural Sciences Uppsala Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helena K.","family":"Pettersson","sequence":"additional","affiliation":[{"name":"Clinical Pathology Laboratory University Animal Hospital, Swedish University of the Agricultural Sciences Uppsala Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gad","family":"Baneth","sequence":"additional","affiliation":[{"name":"Koret School of Veterinary Medicine Hebrew University Rehovot Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,6,27]]},"reference":[{"key":"e_1_2_4_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-017-2063-2"},{"key":"e_1_2_4_3_1","unstructured":"Swedish Veterinary Institute Homepage: Information AboutHepatozoon(in Swedish). 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