{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T23:38:01Z","timestamp":1773790681152,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"S1","license":[{"start":{"date-parts":[[2019,7,18]],"date-time":"2019-07-18T00:00:00Z","timestamp":1563408000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007879","name":"Thure F och Karin Forsbergs Stiftelse","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007879","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Canine anti\u2010mouse antibodies are a potential source of immunoassay interference, but erroneous immunoassay results are not always easily identifiable. Anti\u2010M\u00fcllerian hormone (AMH) is a marker for the presence of gonads in dogs, but elevated AMH concentrations in neutered dogs could also be caused by antibody interference. For other assays, a discrepant result obtained after antibody precipitation might indicate antibody interference.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>We aimed to evaluate if canine anti\u2010mouse antibodies are a source of erroneous results in the AMH assay and if antibody precipitation with polyethylene glycol (PEG) is a useful tool for detecting antibody interference in a variety of immunoassays used in the veterinary clinical laboratory.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Twenty\u2010nine positive and 25 negative samples for anti\u2010mouse antibodies were analyzed for AMH, canine total thyroxine (TT<jats:sub>4<\/jats:sub>), canine thyroid\u2010stimulating hormone (TSH) and progesterone before and after treatment with PEG. Results that differed by more than four SDs from the intra\u2010assay coefficients of variation were considered discrepant. Elevated AMH concentrations in neutered dogs with anti\u2010mouse antibodies and no visible gonads present were considered evidence of interference.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Evidence of antibody interference was found in two samples analyzed for AMH. The presence of anti\u2010mouse antibodies did not lead to a higher proportion of discrepant results after PEG treatment for any of the immunoassays. The overall incidence of discrepant results for healthy controls was very high (73%).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Canine anti\u2010mouse antibodies are a source of erroneous AMH results. Antibody precipitation with PEG is not a useful tool for detecting interference caused by such antibodies.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vcp.12764","type":"journal-article","created":{"date-parts":[[2019,7,18]],"date-time":"2019-07-18T09:09:32Z","timestamp":1563440972000},"page":"59-69","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Investigation of interference from canine anti\u2010mouse antibodies in hormone immunoassays"],"prefix":"10.1111","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2492-5107","authenticated-orcid":false,"given":"Daniel","family":"Bergman","sequence":"first","affiliation":[{"name":"Department of Clinical Sciences Swedish University of Agricultural Sciences Uppsala Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anders","family":"Larsson","sequence":"additional","affiliation":[{"name":"Department of Medical Sciences Uppsala University Uppsala Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helene","family":"Hansson\u2010Hamlin","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences Swedish University of Agricultural Sciences Uppsala Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bodil","family":"Str\u00f6m Holst","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences Swedish University of Agricultural Sciences Uppsala Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,7,18]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.12612"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2017.09.028"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2014.11.016"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2016.05.004"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11259-015-9639-0"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/1040638711403428"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12856"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2012.12.037"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2007.152"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1373\/49.10.1708"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1258\/acb.2009.009059"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/45.5.616"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1515\/cclm-2012-0038"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.102611"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/vde.12112"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcpa.2011.03.011"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmt062"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-015-0487-5"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.13171"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.12822"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/48.4.613"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/108.4.417"},{"key":"e_1_2_7_24_1","doi-asserted-by":"crossref","first-page":"2023","DOI":"10.1093\/clinchem\/48.11.2023","article-title":"Wrong biochemistry results: two case reports and observational study in 5310 patients on potentially misleading thyroid\u2010stimulating hormone and gonadotropin immunoassay results","volume":"48","author":"Ismail AA","year":"2002","journal-title":"Clin Chem"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0009-8981(02)00275-9"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.2008.00002.x"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2004.042523"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.beem.2013.05.011"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1177\/0004563213487514"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(99)00206-9"},{"key":"e_1_2_7_31_1","first-page":"1165","article-title":"Human\u2010Igm rheumatoid\u2010factor reactivity with rabbit, sheep","volume":"34","author":"Hamilton RG","year":"1988","journal-title":"Goat and Mouse Immunoglobulin. 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