{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T19:02:24Z","timestamp":1790535744300,"version":"4.1.0"},"reference-count":13,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,10,24]],"date-time":"2019-10-24T00:00:00Z","timestamp":1571875200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The appropriate cutoff to define a positive point\u2010of\u2010care card agglutination (CA) test for dog erythrocyte antigen 1 (DEA 1) blood typing depends on whether the test can be used in the donor or recipient.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>By screening for CA test positivity, we aimed to evaluate the best cutoff value for DEA 1 blood typing in canine blood donors\u00a0using a receiver operator characteristic (ROC) curve.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Ethylenediaminetetraacetic acid (EDTA) blood samples from 100 canine blood donors were blood\u2010typed in parallel for DEA 1 using both immunochromatographic (IC) and CA tests. The effect of temperature, storage time, and anticoagulant solutions for both methods was evaluated. Unweighted and weighted Cohen's Kappa (<jats:italic>K<\/jats:italic>) statistic was calculated to evaluate the agreement between the two testing methods. The overall performance of the CA test was evaluated by generating a ROC curve using the IC test as the reference method.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Concordant results were obtained for 86% of the samples. Unweighted and weighted <jats:italic>K<\/jats:italic> statistics demonstrated good\u00a0and moderate\u00a0agreement, respectively. For the CA test, the ROC curve showed an area under the curve (AUC) of 0.910, with the highest sensitivity cutoff values at \u22651+ agglutination. CA\u2010 and IC\u2010typed EDTA blood samples stored at room temperature for up to 1 week and refrigerated for up to 1 month were concordant as were the citrate phosphate dextrose adenine 1 (CPDA\u20101) anticoagulated blood samples stored for up to 1 week at 4\u00a0\u00b1\u00a02\u00b0C.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The overall reliability of the CA method seemed to be lower than that of the IC method. When CA is used as a screening test for canine blood donors, the correct cut off is \u22651+ agglutination is recommended to maximize sensitivity.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vcp.12788","type":"journal-article","created":{"date-parts":[[2019,10,25]],"date-time":"2019-10-25T06:58:26Z","timestamp":1571986706000},"page":"630-635","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["A card agglutination test for dog erythrocyte antigen 1 (DEA 1)\u00a0blood typing in donor dogs: Determining an appropriate cutoff to detect positivity using a receiver operating characteristic curve"],"prefix":"10.1111","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3922-6069","authenticated-orcid":false,"given":"Daniela","family":"Proverbio","sequence":"first","affiliation":[{"name":"Veterinary Research Transfusion Laboratory (REVLab) Department of Veterinary Medicine (DiMeVet) University of Milan Milan 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