{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T08:59:28Z","timestamp":1780477168693,"version":"3.54.1"},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,3,3]],"date-time":"2020-03-03T00:00:00Z","timestamp":1583193600000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Knowledge of mammalian inflammatory responses is vast; however, many aspects of the inflammatory response in non\u2010mammalian vertebrates, such as reptiles, remain unclear, including those regarding acute\u2010phase proteins (APPs). Recent studies have focused on the use of serum protein electrophoresis (SPE) to assess inflammatory responses in the broad\u2010snouted caiman (<jats:italic>Caiman latirostris<\/jats:italic>) and other reptiles.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>The purpose of this study was to examine the effects of sex, body length, and different habitats on SPE patterns in <jats:italic>C\u00a0latirostris<\/jats:italic> using sodium dodecyl sulfate\u2010polyacrylamide gel electrophoresis (SDS\u2010PAGE).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 40 animals of both sexes and varying body lengths were collected; of these, 23 were free\u2010living in an industrial complex (site 1), and 17 were captive on a rural property (site 2). Sodium dodecyl sulfate\u2010polyacrylamide gel electrophoresis was performed to fractionate different serum protein constituents.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Sex affected protein levels, with females showing higher serum levels of total protein, as well as the 90\u2010, 66\u2010, and 58\u2010kDa proteins compared with males. Similarly, body length affected protein levels, with male adults &gt;1.70\u00a0m in length showing the lowest serum levels of 152\u2010, 58\u2010, 54\u2010, and 27\u2010kDa proteins of all other animals. Finally, habitat affected protein levels, with animals at site 1 showing higher serum levels of 152\u2010 and 41\u2010kDa proteins compared with those at site 2.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This preliminary study was the first to evaluate the SPE of <jats:italic>C\u00a0latirostris<\/jats:italic> using SDS\u2010PAGE. Further studies to identify the proteins in each band with more specific and sensitive techniques (eg, mass spectrometry) should be conducted to elucidate the standard of APPs in reptiles better.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vcp.12827","type":"journal-article","created":{"date-parts":[[2020,3,4]],"date-time":"2020-03-04T07:06:02Z","timestamp":1583305562000},"page":"59-65","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The effects of sex, body length, and different habitats on the serum protein electrophoresis of the broad\u2010snouted caiman (<i>Caiman latirostris<\/i>)"],"prefix":"10.1111","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5296-6657","authenticated-orcid":false,"given":"Leandro A.","family":"Fonseca","sequence":"first","affiliation":[{"name":"Professor of Veterinary Clinical Pathology in Veterinary Department Federal University of Vi\u00e7osa Vi\u00e7osa Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorraine R. S. M.","family":"Dornelas","sequence":"additional","affiliation":[{"name":"Veterinary Department Federal University of Vi\u00e7osa Vi\u00e7osa Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andres M. O.","family":"Orozco","sequence":"additional","affiliation":[{"name":"Veterinary Department Federal University of Vi\u00e7osa Vi\u00e7osa Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pollyanna C.","family":"Souto","sequence":"additional","affiliation":[{"name":"Veterinary Department Federal University of Vi\u00e7osa Vi\u00e7osa Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas D.","family":"Bento","sequence":"additional","affiliation":[{"name":"Veterinary Department Federal University of Vi\u00e7osa Vi\u00e7osa Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yhuri C.","family":"N\u00f3brega","sequence":"additional","affiliation":[{"name":"Projeto Caiman Marcos Daniel Institute Vit\u00f3ria Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo R. 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