{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:24:10Z","timestamp":1787016250679,"version":"build-2736575974"},"reference-count":38,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,4,4]],"date-time":"2020-04-04T00:00:00Z","timestamp":1585958400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Flow cytometry (FC) is used increasingly in veterinary medicine for further characterization of hematolymphoid cells. Guidelines for optimizing assay performance and interpretation of results are limited, and concordance of results across laboratories is unknown.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>This study aimed to determine inter\u2010investigator agreement on the interpretation of FC results from split samples analyzed in different laboratories using various protocols, cytometers, and software; and on the interpretation of archived FC standard (FCS) data files contributed by the different investigators.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This was a multicenter observational cross\u2010sectional study. Anticoagulated blood or lymph node aspirate samples from nine client\u2010owned dogs were aliquoted and shipped to participating laboratories. Samples were analyzed with individual laboratory\u2010developed protocols. In addition, FCS files from a set of separate samples from 11 client\u2010owned dogs were analyzed by participating investigators. A person not associated with the study tabulated the results and interpretations. Agreement of interpretations was assessed with Fleiss\u2019 kappa statistic.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Prolonged transit times affected sample quality for some laboratories. Overall agreement among investigators regarding the FC sample interpretation was strong (\u03ba\u00a0=\u00a00.86\u00a0\u00b1\u00a00.19,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a0.001), and for specific categories, ranged from moderate to perfect. Agreement of the lymphoproliferation or other leukocyte sample category from the analysis of the FCS files was weak (\u03ba\u00a0=\u00a00.58\u00a0\u00b1\u00a00.05,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a0.001).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Lymphoproliferations were readily identified by FC, but identification of the categories of hematolymphoid neoplasia in fresh samples or archived files was variable. There is a need for a more standardized approach to maximize the enormous potential of FC in veterinary medicine.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/vcp.12843","type":"journal-article","created":{"date-parts":[[2020,4,4]],"date-time":"2020-04-04T04:04:18Z","timestamp":1585973058000},"page":"249-257","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Multicenter flow cytometry proficiency testing of canine blood and lymph node samples"],"prefix":"10.1111","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8982-2697","authenticated-orcid":false,"given":"Kristina","family":"Meichner","sequence":"first","affiliation":[{"name":"Department of Pathology College of Veterinary Medicine University of Georgia Athens GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9697-638X","authenticated-orcid":false,"given":"Tracy","family":"Stokol","sequence":"additional","affiliation":[{"name":"Department of Population Medicine and Diagnostic Sciences Cornell University Ithaca NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaime","family":"Tarigo","sequence":"additional","affiliation":[{"name":"Department of Pathology College of Veterinary Medicine University of Georgia Athens GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Avery","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology &amp; Pathology University of Colorado Fort Collins CO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary J.","family":"Burkhard","sequence":"additional","affiliation":[{"name":"Department of Veterinary Biosciences The Ohio State University Columbus OH USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1567-8055","authenticated-orcid":false,"given":"Stefano","family":"Comazzi","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine University of Milan Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3837-6719","authenticated-orcid":false,"given":"Jonathan","family":"Fogle","sequence":"additional","affiliation":[{"name":"Department of Population Health and Pathobiology North Carolina State University Raleigh NC USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4058-2995","authenticated-orcid":false,"given":"Devorah Marks","family":"Stowe","sequence":"additional","affiliation":[{"name":"Department of Population Health and Pathobiology North Carolina State University Raleigh NC USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"R\u00fctgen","sequence":"additional","affiliation":[{"name":"University of Vienna Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Davis","family":"Seelig","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences University of Minnesota St Paul MN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adi","family":"Wasserkrug\u2010Naor","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Medicine\/Pathobiology Kansas State University Manhattan KS USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William","family":"Vernau","sequence":"additional","affiliation":[{"name":"Department of Pathology, Microbiology &amp; 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