{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T02:07:40Z","timestamp":1747188460717,"version":"3.40.5"},"reference-count":17,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,7,11]],"date-time":"2020-07-11T00:00:00Z","timestamp":1594425600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Accurate measurement of fibrinogen is necessary for detecting bleeding tendencies and inflammation. The Clauss assay determines fibrinogen concentration from its inverse relationship with thrombin\u2010induced clot times. PT\u2010derived assays determine fibrinogen concentrations from changes in the optical density during a routine prothrombin assay and allow determination of fibrinogen without additional reagents. This method has not been assessed in clinically ill dogs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>We aimed to determine the agreement between the Clauss and PT\u2010derived fibrinogen assays and compare the ability of the assays to predict surgery\u2010associated transfusions and discriminate between dogs with and without bleeding.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Retrospective medical record review identified 200 dogs with a variety of underlying diseases with results from both assays. The two assays were compared using Passing\u2010Bablok regression, and the ability of the assays to identify bleeding and predict the need for transfusions was assessed with receiver operating characteristic (ROC) curve analyses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The PT\u2010derived assay displayed constant (<jats:italic>y<\/jats:italic>\u2010intercept, 32\u00a0mg\/dL; 95% CI 18\u201041) and proportional (slope, 0.79; 95% CI 0.75\u20100.82) bias compared with the Clauss assay. The Clauss assay reported lower values than the PT\u2010derived assay at lower fibrinogen concentrations and higher values at higher concentrations. Comparing the area under the ROC curve did not detect significant differences in the ability of the two assays to discriminate between dogs with and without bleeding or predict the need for surgery\u2010associated transfusions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The PT\u2010derived and Clauss assays are not interchangeable, and the Clauss assay could be more sensitive to hypofibrinogenemia in dogs.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vcp.12878","type":"journal-article","created":{"date-parts":[[2020,7,12]],"date-time":"2020-07-12T02:54:10Z","timestamp":1594522450000},"page":"394-400","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparison of the prothrombin time\u2010derived and Clauss assays for the measurement of plasma fibrinogen in hospitalized dogs"],"prefix":"10.1111","volume":"49","author":[{"given":"Michelle H.","family":"Hasiuk","sequence":"first","affiliation":[{"name":"Department of Small Animal Clinical Sciences College of Veterinary Medicine &amp; Biomedical Sciences Texas A&amp;M University College Station TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen E.","family":"Russell","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology College of Veterinary Medicine &amp; Biomedical Sciences Texas A&amp;M University College Station TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0495-2741","authenticated-orcid":false,"given":"Unity","family":"Jeffery","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology College of Veterinary Medicine &amp; Biomedical Sciences Texas A&amp;M University College Station TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,7,11]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.14723"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/vec.12590"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-5827.2011.01139.x"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-017-1110-8"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.14626"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2016.03.027"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0036-1579636"},{"key":"e_1_2_5_9_1","first-page":"864","article-title":"Determination of plasma fibrinogen concentrations in beagle dogs, cynomolgus monkeys, New Zealand white rabbits, and Sprague\u2010Dawley rats by using Clauss and prothrombin\u2010time\u2010derived assays","volume":"50","author":"Ameri M","year":"2011","journal-title":"J Am Assoc Lab Anim Sci"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2006.05.003"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2011-0322-OA"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.injury.2017.01.031"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.blre.2014.09.003"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/rth2.12107"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2010.09.004"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001721-199412000-00012"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvsm.2016.11.009"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001721-199804000-00006"}],"container-title":["Veterinary Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fvcp.12878","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vcp.12878","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/vcp.12878","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vcp.12878","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,4]],"date-time":"2023-09-04T18:32:32Z","timestamp":1693852352000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vcp.12878"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,7,11]]},"references-count":17,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2020,9]]}},"alternative-id":["10.1111\/vcp.12878"],"URL":"https:\/\/doi.org\/10.1111\/vcp.12878","archive":["Portico"],"relation":{},"ISSN":["0275-6382","1939-165X"],"issn-type":[{"type":"print","value":"0275-6382"},{"type":"electronic","value":"1939-165X"}],"subject":[],"published":{"date-parts":[[2020,7,11]]},"assertion":[{"value":"2019-03-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-11-14","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-07-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}