{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T11:41:29Z","timestamp":1785498089044,"version":"3.56.0"},"reference-count":8,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,1,5]],"date-time":"2021-01-05T00:00:00Z","timestamp":1609804800000},"content-version":"vor","delay-in-days":35,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100001250","name":"Morris Animal Foundation","doi-asserted-by":"publisher","award":["#D18FE\u2010011"],"award-info":[{"award-number":["#D18FE\u2010011"]}],"id":[{"id":"10.13039\/100001250","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Feline Infectious Peritonitis (FIP) is a fatal disease of cats that can be very difficult to definitively diagnose antemortem. Multiplex fluorescent immunocytochemical (MF\u2010ICC) assays are emerging as useful diagnostic tests in veterinary medicine, particularly for fluid samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>We aimed to develop and optimize an MF\u2010ICC assay to detect feline coronavirus within macrophages, with the primary goal of determining the allowable\/recommended sample storage conditions for clinical use of this assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A feline macrophage cell line was infected with the FIP virus. Following harvest into EDTA tubes (simulating typical clinical collection of effusion), cells were stored at 4\u2103, 22\u2103, and 37\u2103. For each temperature condition, slides for MF\u2010ICC were made at 0, 1, 2, 3, and 5\u00a0days post\u2010collection. To assess the stability of immunoreactivity following fixation, freshly harvested infected cells were fixed onto slides and maintained at 4\u2103 for 1, 2, 4, and 12\u00a0weeks. All slides were analyzed by MF\u2010ICC for the presence of mononuclear cells with co\u2010expression of vimentin and coronaviral antigen.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>MF\u2010ICC confirmed that cells tested positive for coronavirus at 4\u2103 through 3\u00a0days post\u2010harvest, 22\u2103 through 48\u00a0hours post\u2010harvest, and 37\u2103 through 24\u00a0hours post\u2010harvest. The MF\u2010ICC assay was successfully performed on fixed slides through the 12\u2010week time point. This assay also demonstrated positive results on a clinical sample of abdominal fluid from a cat later confirmed to have FIP.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The MF\u2010ICC assay described here offers a potentially specific and relatively stable antemortem diagnostic test for feline infectious peritonitis. Evaluation of this assay in clinical samples is ongoing.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vcp.12912","type":"journal-article","created":{"date-parts":[[2021,1,7]],"date-time":"2021-01-07T04:14:43Z","timestamp":1609992883000},"page":"640-645","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Multiplex fluorescent immunocytochemistry for the diagnosis of feline infectious peritonitis: Determining optimal storage conditions"],"prefix":"10.1111","volume":"49","author":[{"given":"Margaret","family":"Howell","sequence":"first","affiliation":[{"name":"Department of Microbiology, Immunology, and Pathology College of Veterinary Medicine and Biomedical Science Colorado State University Fort Collins CO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2182-0684","authenticated-orcid":false,"given":"Samantha J. M.","family":"Evans","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Pathology College of Veterinary Medicine and Biomedical Science Colorado State University Fort Collins CO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle","family":"Cornwall","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Pathology College of Veterinary Medicine and Biomedical Science Colorado State University Fort Collins CO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelly S.","family":"Santangelo","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Pathology College of Veterinary Medicine and Biomedical Science Colorado State University Fort Collins CO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,1,5]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2014.04.016"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/1098612X16630357"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/0300985814522077"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2014.04.017"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2013.08.023"},{"issue":"2","key":"e_1_2_6_7_1","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.jfms.2004.01.001","article-title":"Evaluation of an in\u2010practice test for feline coronavirus antibodies","volume":"6","author":"Addie DD","year":"2004","journal-title":"J Feline Med Surg"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.12394"},{"key":"e_1_2_6_9_1","volume-title":"Fcwf\u20104 [Fcwf] ATCC \u00ae CRL\u20102787TM Felis catus fetus, whole peri","author":"American Type Culture Collection","year":"2018"}],"container-title":["Veterinary Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vcp.12912","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/vcp.12912","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vcp.12912","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,3]],"date-time":"2023-09-03T12:10:31Z","timestamp":1693743031000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vcp.12912"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,12]]},"references-count":8,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2020,12]]}},"alternative-id":["10.1111\/vcp.12912"],"URL":"https:\/\/doi.org\/10.1111\/vcp.12912","archive":["Portico"],"relation":{},"ISSN":["0275-6382","1939-165X"],"issn-type":[{"value":"0275-6382","type":"print"},{"value":"1939-165X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,12]]},"assertion":[{"value":"2019-12-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-04-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-01-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}