{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T00:34:12Z","timestamp":1774658052821,"version":"3.50.1"},"reference-count":50,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,12,5]],"date-time":"2020-12-05T00:00:00Z","timestamp":1607126400000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Hemotherapy in ruminants is limited to whole blood transfusions, sometimes with stored blood for up to 42\u00a0days, but little attention has been given to the effect of blood storage times and recipient responses after transfusions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>We aimed to evaluate the hematologic and serum biochemical effects after allogeneic blood transfusion with either fresh or stored blood in sheep. We also sought to examine hematologic and biochemical analyte changes in the store blood.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Eighteen sheep underwent a single phlebotomy to remove 40% of their blood volume. The sheep were divided into three experimental groups, G0, G15, and G35, which included six animals, each receiving 20\u00a0mL\/kg of either fresh blood or blood stored in citrate, phosphate, dextrose, and adenine (CPDA\u20101) bags for 15 and 35\u00a0days, respectively. Biochemical, hematologic, coagulation, blood gas, lipid peroxidation, and oxidative stress test evaluations were performed using the blood samples gathered at T0 (before transfusion), 30\u00a0minutes (T30m), 6, 12, 24, 48, 72, and 96\u00a0hours (T6h\u2010T96h), 8\u00a0days (T8d), and 16\u00a0days (T16d) after transfusions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Sheep exhibited increases in packed cell volumes, red blood cell counts, and total hemoglobin concentrations at T30m (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.05). G35 animals had greater plasma hemoglobin concentrations at T12h and decreased blood pH values at T6h, characterized by slight metabolic acidemia. Regarding oxidative stress, G35 animals had decreased catalase activities from T0 at T30m, T6h, T12h, and T24h, indicating that hemolysis had occurred, which was supported by concomitant increases in bilirubin.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Sheep transfused with 35\u2010day stored blood exhibited greater hematologic, blood gas, biochemical, and oxidative alterations; however, anemic animals without comorbidities effectively reversed those alterations.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vcp.12917","type":"journal-article","created":{"date-parts":[[2020,12,5]],"date-time":"2020-12-05T12:26:19Z","timestamp":1607171179000},"page":"545-556","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Impact of blood storage duration on hematologic, blood gas, biochemical, and oxidative stress variables in sheep undergoing allogeneic blood transfusions"],"prefix":"10.1111","volume":"49","author":[{"given":"Rejane S.","family":"Sousa","sequence":"first","affiliation":[{"name":"Federal University of the South and Southeast of Par\u00e1 (Unifesspa) Xinguara Brazil"},{"name":"Federal Rural University of the Semi\u2010Arid Zone Mossor\u00f3 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6742-3652","authenticated-orcid":false,"given":"Antonio H. 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