{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T20:15:25Z","timestamp":1778876125616,"version":"3.51.4"},"reference-count":40,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,1,11]],"date-time":"2021-01-11T00:00:00Z","timestamp":1610323200000},"content-version":"vor","delay-in-days":41,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Decreased reticulocyte hemoglobin content (CHr) (Siemens ADVIA 2120) reflects iron\u2010limited erythropoiesis (ILE). RETIC\u2010HGB (IDEXX ProCyte Dx) is a novel marker of ILE for veterinary use.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>We aimed to evaluate reference intervals (RIs) and the utility of RETIC\u2010HGB and CHr in the diagnosis of feline ILE.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>RIs were established in 59 healthy cats. Intra\u2010assay coefficients of variation (CVs) and correlations between RETIC\u2010HGB and CHr were assessed. Two hundred and seventy\u2010five cats were classified as having ILE or not based on low plasma iron or low transferrin saturation along with anemia and\/or altered RBC indices. CHr, RETIC\u2010HGB, and serum amyloid A (SAA) were compared between the groups. The sensitivity and specificity of RETIC\u2010HGB and CHr to diagnose ILE were analyzed to determine the RI lower limits.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>RIs for RETIC\u2010HGB and CHr were 12.5\u201018.0 and 14.0\u201019.9\u00a0pg, respectively. The CV was 3% for both variables. RETIC\u2010HGB and CHr were moderately correlated (<jats:italic>r<\/jats:italic><jats:sub>s<\/jats:sub>\u00a0=\u00a00.59) with a bias of \u22121.2 picograms (pgs). Twenty of the 275 cats were classified as having ILE. Compared with non\u2010ILE cats, ILE cats had significantly lower median RETIC\u2010HGB (14.3 vs 15.2\u00a0pg, <jats:italic>P<\/jats:italic>\u00a0=\u00a0.0046) and mean CHr (14.7 vs 16.5\u00a0pg, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.0001) values and significantly increased median SAA (44.6 vs 2.3\u00a0\u00b5g\/dl, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.0001) values. Using the lower RI limits resulted in a low sensitivity and relatively high specificity to diagnose ILE in cats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>ILE was characterized by decreased CHr and RETIC\u2010HGB; however, sensitivity was low. The moderate correlation between RETIC\u2010HGB and CHr is likely due to species differences and different methodology.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vcp.12925","type":"journal-article","created":{"date-parts":[[2021,1,15]],"date-time":"2021-01-15T04:25:22Z","timestamp":1610684722000},"page":"557-566","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Evaluation of reticulocyte hemoglobin content (RETIC\u2010HGB) for the diagnosis of iron\u2010limited erythropoiesis in cats"],"prefix":"10.1111","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5025-9174","authenticated-orcid":false,"given":"Miriam","family":"Keiner","sequence":"first","affiliation":[{"name":"Small Animal Clinic Internal Medicine Justus\u2010Liebig\u2010University Giessen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jannika","family":"Fuchs","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences, Clinical Pathology and Clinical Pathophysiology Justus\u2010Liebig\u2010University Giessen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natali","family":"Bauer","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences, Clinical Pathology and Clinical Pathophysiology Justus\u2010Liebig\u2010University Giessen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreas","family":"Moritz","sequence":"additional","affiliation":[{"name":"Small Animal Clinic Internal Medicine Justus\u2010Liebig\u2010University Giessen Germany"},{"name":"Department of Veterinary Clinical Sciences, Clinical Pathology and Clinical Pathophysiology Justus\u2010Liebig\u2010University Giessen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,1,11]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1373\/49.10.1573"},{"key":"e_1_2_7_3_1","first-page":"40","volume-title":"Canine and Feline Nutrition: A Resource for Companion Animal Professionals","author":"Case LP","year":"2010"},{"key":"e_1_2_7_4_1","article-title":"Anemia of chronic disease","volume":"134","author":"Weiss G","year":"2005","journal-title":"N Engl J Med"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.2005.tb00004.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.1997.2123031.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-553X.2009.01160.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2011.03495.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcpa.2015.01.008"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.12219"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1532\/LH96.04049"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.2006.tb00110.x"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.12311"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOH.0b013e32835f5933"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2010.292"},{"issue":"2","key":"e_1_2_7_16_1","doi-asserted-by":"crossref","first-page":"223","DOI":"10.2478\/folmed-2018-0065","article-title":"Diagnostic significance of biomarkers of iron deficiency for anemia in clinical practice","volume":"61","author":"Petkova NY","year":"2019","journal-title":"Folia Med"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00431-018-3257-0"},{"issue":"7","key":"e_1_2_7_18_1","first-page":"272","article-title":"Stability and reproducibility of ADVIA 120\u2010measured red blood cell and platelet parameters in dogs, cats, and horses, and the use of reticulocyte haemoglobin content (CHR) in the diagnosis of iron deficiency","volume":"134","author":"Prins M","year":"2009","journal-title":"Tijdschr Diergeneeskd"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2257.2006.00812.x"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.12499"},{"key":"e_1_2_7_21_1","unstructured":"IDEXX Laboratories.Reference intervals for the IDEXX ProCyte Dx*. Hematology Analyzer.2019.https:\/\/www.idexx.com\/files\/procyte\u2010dx\u2010reference\u2010ranges\u2010en.pdf"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.2011.00287.x"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.12006"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/36.2.265"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0195-5616(03)00099-8"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1080\/00365510500406910"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11259-006-3373-6"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1177\/1040638711436243"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3287615"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.12547"},{"issue":"4","key":"e_1_2_7_31_1","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1111\/j.1939-1676.2010.0541.x","article-title":"Breed dependency of reference intervals for plasma biochemical values in cats: reference intervals for cats","volume":"24","author":"Reynolds BS","year":"2010","journal-title":"J Vet Intern Med"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfms.2008.06.010"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-017-1138-9"},{"issue":"1","key":"e_1_2_7_34_1","first-page":"39","article-title":"A comparison of polychromasia and reticulocyte counts in assessing erythrocytic regenerative response in the cat","volume":"170","author":"Alsaker RD","year":"1977","journal-title":"J Am Vet Med Assoc"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.12583"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.2009.00125.x"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1177\/030098589202900603"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-08-2459"},{"issue":"5","key":"e_1_2_7_39_1","first-page":"389","article-title":"Predictive value of absent bone marrow iron stores in the clinical diagnosis of iron deficiency anemia","volume":"17","author":"Ganti AK","year":"2003","journal-title":"In Vivo"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvsm.2013.07.002"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1503\/cmaj.121286"}],"container-title":["Veterinary Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vcp.12925","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/vcp.12925","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vcp.12925","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,3]],"date-time":"2023-09-03T12:09:41Z","timestamp":1693742981000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vcp.12925"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,12]]},"references-count":40,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2020,12]]}},"alternative-id":["10.1111\/vcp.12925"],"URL":"https:\/\/doi.org\/10.1111\/vcp.12925","archive":["Portico"],"relation":{},"ISSN":["0275-6382","1939-165X"],"issn-type":[{"value":"0275-6382","type":"print"},{"value":"1939-165X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,12]]},"assertion":[{"value":"2019-10-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-04-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-01-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}