{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T16:12:16Z","timestamp":1762272736418,"version":"3.40.5"},"reference-count":42,"publisher":"Wiley","issue":"S1","license":[{"start":{"date-parts":[[2021,5,24]],"date-time":"2021-05-24T00:00:00Z","timestamp":1621814400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Hepatic cholesterol accumulation in small breed dogs is a leading risk factor for hepatic fatty changes, gallbladder hypomotility, and cholelith development, which, if not discovered early, could lead to life\u2010threatening choledocholithiasis and acute pancreatitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>This study proposed to assess the use of hepatocyte\u2010derived <jats:italic>canine familiaris<\/jats:italic> (cfa)\u2010microRNAs (miRNA\u2010122, \u201034a, and \u201021) as new diagnostic serum biomarkers of liver steatosis or fibrosis, for which both processes have been implicated in canine cholecystolithiasis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Forty client\u2010owned dogs diagnosed with cholecystolithiasis and hepatic steatosis (C+HS) or fibrosis (C+HF) based on ultrasonographic, biochemical, and histopathologic findings, and 20 healthy dogs used as controls were included in the study. Serum cfa\u2010miRNA expression was determined using a real\u2010time polymerase chain reaction assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Serum cfa\u2010miRNA\u2010122 and \u221234a expression was significantly upregulated in the C+HS (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.001) and C+HF (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.01) groups compared with the control group and showed a positive correlation with alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBIL), alkaline phosphatase (ALP), \u03b3\u2010glutamyl transferase (GGT), total cholesterol (TC), and triglycerides (TG) levels in the C+HS group. Cfa\u2010miRNA\u2010122 and \u221234a expression discriminated the diseased groups from the control group better\u00a0than traditional serum\u2010derived liver biomarkers, as evidenced by areas under the receiver operating characteristic (AUC\u2010ROC) curve of 0.99 and 0.97 for cfa\u2010miRNA\u2010122 expression in the C+HS and C+HF groups, and 1.0 and 0.96 for cfa\u2010miRNA\u201034a in the C+HS and C+HF groups, respectively. Cfa\u2010miRNA\u201021 expression was upregulated only in the C+HF group compared with the C+HS (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.01) and control (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.001) groups and showed a positive correlation with serum ALT, AST, TBIL, ALP, and GGT and negative correlation with serum TC and TG levels. Cfa\u2010miRNA\u201021 expression could also differentiate the C+HF group from the control and C+HS groups with a diagnostic performance superior to that of the conventional serum biochemical variables as evidenced by AUCs of 1.0 and 0.98, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Serum cfa\u2010miRNA\u2010122, \u201034a, and \u201021 expression was significantly upregulated in dogs with cholecystolithiasis with hepatic steatosis or fibrosis compared with control dogs. These miRNAs could serve as novel biomarkers for hepatic steatosis or fibrosis, which have been implicated in the pathogenesis of cholecystolithiasis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vcp.12942","type":"journal-article","created":{"date-parts":[[2021,5,25]],"date-time":"2021-05-25T01:49:45Z","timestamp":1621907385000},"page":"37-46","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Hepatocyte\u2010derived <i>canine familiaris<\/i>\u2010microRNAs as serum biomarkers of hepatic steatosis or fibrosis as implicated in the pathogenesis of canine cholecystolithiasis"],"prefix":"10.1111","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1837-3182","authenticated-orcid":false,"given":"Ahmed M.","family":"El\u2010Sebaey","sequence":"first","affiliation":[{"name":"Department of Clinical Pathology Faculty of Veterinary Medicine Mansoura University  Mansoura Egypt"},{"name":"Department of Disease Diagnosis, Therapy, Obstetrics, and Animal Reproduction Moscow State Academy of Veterinary Medicine and Biotechnology \u2013 MVA by K. 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