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In this condition, a functional \u03b2\u2010glucuronidase deficiency results in the accumulation of glycosaminoglycans (GAGs) in cells and tissues where \u03b2\u2010glucuronidase is important in GAG degradation. Most neutrophils and a moderate number of lymphocytes within the blood had atypical cytoplasmic magenta inclusions. The bone marrow assessment from one of the two affected pups at 24\u00a0days of age revealed similar magenta granulation in myeloid precursor cells that was most prominent in promyelocytes and myelocytes. Moreover, atypical magenta material was present within vacuoles as well as extracellularly in some osteoblasts and macrophages. Histologic bone marrow sections revealed prominent vacuolation of osteoblasts, and some osteoclasts appeared separated from the bone by layers of osteoblasts or hematopoietic cells. At 2\u00a0months of age, the second affected dog showed moderate growth retardation and had similar but more prominent hematologic findings that extended to monocytes, eosinophils, and eosinophil precursors. It had an increased number of bone marrow macrophages with many vacuoles that could be seen cytologically to contain magenta material, and there was mild nonselective phagocytosis of hemic cells. Of the hematologic cells, inclusions were most prominent in promyelocytes, myelocytes, and macrophages, cells with relatively high \u03b2\u2010glucuronidase activity, and GAG exposure within lysosomes or lysosome\u2010like primary granules of granulocyte precursors.<\/jats:p>","DOI":"10.1111\/vcp.12963","type":"journal-article","created":{"date-parts":[[2021,3,15]],"date-time":"2021-03-15T02:25:20Z","timestamp":1615775120000},"page":"164-169","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Blood and bone marrow findings in two pups with mucopolysaccharidosis type VII"],"prefix":"10.1111","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8032-0327","authenticated-orcid":false,"given":"Erica","family":"Corda","sequence":"first","affiliation":[{"name":"Department of Pathobiology and Diagnostic Investigation College of Veterinary Medicine Michigan State University East Lansing MI USA"},{"name":"Michigan State University Veterinary Diagnostic Laboratory College of Veterinary Medicine Michigan State University East Lansing MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheryl L.","family":"Swenson","sequence":"additional","affiliation":[{"name":"Department of Pathobiology and Diagnostic Investigation College of Veterinary Medicine Michigan State University East Lansing MI USA"},{"name":"Michigan State University Veterinary Diagnostic Laboratory College of Veterinary Medicine Michigan State University East Lansing MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael A.","family":"Scott","sequence":"additional","affiliation":[{"name":"Department of Pathobiology and Diagnostic Investigation College of Veterinary Medicine Michigan State University East Lansing MI USA"},{"name":"Michigan State University Veterinary Diagnostic Laboratory College of Veterinary Medicine Michigan State University East Lansing MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dodd G.","family":"Sledge","sequence":"additional","affiliation":[{"name":"Department of Pathobiology and Diagnostic Investigation College of Veterinary Medicine Michigan State University East Lansing MI USA"},{"name":"Michigan State University Veterinary Diagnostic Laboratory College of Veterinary Medicine Michigan State University East Lansing MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John C.","family":"Fyfe","sequence":"additional","affiliation":[{"name":"Department of Small Animal Clinical Sciences College of Veterinary Medicine Michigan State University East Lansing MI USA"},{"name":"Laboratory of Comparative Medical Genetics College of Veterinary Medicine Michigan State University East Lansing MI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,3,14]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.2012.00415.x"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1354\/vp.37-5-502"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.14862"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1006\/geno.1997.5189"},{"issue":"9","key":"e_1_2_5_6_1","doi-asserted-by":"crossref","first-page":"1092","DOI":"10.2460\/ajvr.1998.59.09.1092","article-title":"Molecular diagnostic tests for ascertainment of genotype at the mucopolysaccharidosis type VII in dogs","volume":"59","author":"Ray J","year":"1998","journal-title":"Am J Vet Res"},{"issue":"10","key":"e_1_2_5_7_1","first-page":"980","article-title":"\u0392\u2010glucuronidase deficiency in a dog; a model of human mucopolysaccharidosis VII","volume":"18","author":"Haskins ME","year":"1984","journal-title":"Pediatr Res"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(73)80162-3"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1983.00103.x-i1"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V91.3.1044"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymgme.2015.09.008"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201208152"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10024-005-0025-8"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(71)90427-7"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(69)90208-0"},{"key":"e_1_2_5_16_1","first-page":"199","article-title":"Glycosaminoglycans of blood cells and hematopoietic organs. 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