{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T16:04:45Z","timestamp":1785427485433,"version":"3.56.0"},"reference-count":8,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,4,15]],"date-time":"2022-04-15T00:00:00Z","timestamp":1649980800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>An 11\u2010year\u2010old spayed female Basset Hound was presented to the Colorado State University Veterinary Teaching Hospital for evaluation of a 7\u2010week history of intermittent collapse, waxing and waning lethargy, and hyporexia. Abdominal ultrasound revealed a 6\u2010mm hypoechoic splenic nodule that, on cytologic evaluation, revealed marked neutrophilic inflammation with intracellular and extracellular bacterial rods frequently producing oval subterminal to terminal endospores, suggestive of <jats:italic>Clostridium<\/jats:italic>. Matrix\u2010assisted laser desorption\/ionization time\u2010of\u2010flight mass spectrometry (MALDI\u2010TOF) performed on bacteria isolated from this nodule initially identified a <jats:italic>Clostridium<\/jats:italic> species, which was eventually confirmed with 16\u2009s rDNA sequencing. Computed tomography (CT) and exploratory laparotomy subsequently identified a 2.5\u2010cm diameter tubular structure beginning at the caudal aspect of the right kidney and coursing caudally containing gas and fluid, consistent with a retroperitoneal abscess, which was resected and also cultured <jats:italic>Clostridium<\/jats:italic> spp. The dog was discharged 3\u2009days postoperatively and was alive at the time of writing, 7\u00a0months after discharge. This case highlights a previously unrecognized bacterial agent in a retroperitoneal abscess. The use of cytologic evaluation yielded a diagnosis of endospore\u2010forming bacteria suggestive of <jats:italic>Clostridium<\/jats:italic> sooner than culture and histopathology, which allowed for adjustment in the antibiotic protocol.<\/jats:p>","DOI":"10.1111\/vcp.13112","type":"journal-article","created":{"date-parts":[[2022,4,16]],"date-time":"2022-04-16T05:44:48Z","timestamp":1650087888000},"page":"560-564","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Retroperitoneal abscess and splenitis with <i>Clostridium<\/i> spp. in a dog"],"prefix":"10.1111","volume":"51","author":[{"given":"Carly W.","family":"Gregory","sequence":"first","affiliation":[{"name":"Department of Clinical Sciences Colorado State University  Fort Collins Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0327-8537","authenticated-orcid":false,"given":"Kristin M.","family":"Zersen","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences Colorado State University  Fort Collins Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3939-8963","authenticated-orcid":false,"given":"Samantha N.","family":"Schlemmer","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences Colorado State University  Fort Collins Colorado USA"},{"name":"University of Georgia, Department of Pathology  Athens Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua B.","family":"Daniels","sequence":"additional","affiliation":[{"name":"Colorado State University  Fort Collins Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,4,15]]},"reference":[{"key":"e_1_2_4_2_1","doi-asserted-by":"publisher","DOI":"10.5326\/JAAHA-MS-5932"},{"key":"e_1_2_4_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms9071536"},{"key":"e_1_2_4_4_1","first-page":"1603","volume-title":"Veterinary Surgery Small Animal","author":"Kirby BM","year":"2018"},{"key":"e_1_2_4_5_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.tcam.2018.08.007"},{"key":"e_1_2_4_6_1","first-page":"1035","article-title":"The retroperitoneum in dogs: retro\u2010 peritoneal infections","volume":"12","author":"Johnston DE","year":"1990","journal-title":"Compend Contin Educ Pract Vet"},{"key":"e_1_2_4_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/0300985816653989"},{"key":"e_1_2_4_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/vru.12941"},{"key":"e_1_2_4_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-019-4584-0"}],"container-title":["Veterinary Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vcp.13112","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/vcp.13112","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vcp.13112","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,22]],"date-time":"2023-08-22T07:24:04Z","timestamp":1692689044000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vcp.13112"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4,15]]},"references-count":8,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2022,12]]}},"alternative-id":["10.1111\/vcp.13112"],"URL":"https:\/\/doi.org\/10.1111\/vcp.13112","archive":["Portico"],"relation":{},"ISSN":["0275-6382","1939-165X"],"issn-type":[{"value":"0275-6382","type":"print"},{"value":"1939-165X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,4,15]]},"assertion":[{"value":"2021-11-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-01-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-04-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}