{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T01:47:42Z","timestamp":1775094462737,"version":"3.50.1"},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,5,26]],"date-time":"2022-05-26T00:00:00Z","timestamp":1653523200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Samples collected from an 11\u2010month\u2010old Dachshund\u2010mix dog with a history of acute azotemia, fever, and enlarged and irregular kidneys were received at the Colorado State Veterinary Diagnostic Laboratory (CSU VDL). The submitting veterinarians were concerned about lymphoma versus acute nephritis\/pyelonephritis. The CSU clinical pathology laboratory received urine for urinalysis and kidney aspirates for cytologic evaluation. Urine had also been submitted for aerobic culture and Leptospirosis PCR, and serum was submitted for Lepto\u20105 microscopic agglutination testing (MAT). Upon examination of a wet mount of the urine sediment, technical staff noted \u201cvibrating\u201d clumps of granular\u2010appearing material throughout the slide, which prompted the preparation of a stained sediment slide for pathologist review. Very small, faintly staining organisms were observed, and an attempt was made to picture\u2010match these with published reports of <jats:italic>Leptospira<\/jats:italic> in dog urine, but none could be found. In addition, some references claimed that <jats:italic>Leptospira<\/jats:italic> organisms are not seen in urine with light microscopy. The suspicion that these organisms were <jats:italic>Leptospira<\/jats:italic> sp. was supported by the MAT results and later confirmed by PCR. The organisms subsequently exhibited strong positive immunolabeling for the <jats:italic>Leptospira<\/jats:italic> antigen. This case report provides a searchable record of <jats:italic>Leptospira<\/jats:italic> organisms visualized by routine light microscopy in dog urine during natural infection and a review of canine leptospirosis pathobiology and diagnosis.<\/jats:p>","DOI":"10.1111\/vcp.13129","type":"journal-article","created":{"date-parts":[[2022,5,27]],"date-time":"2022-05-27T04:45:03Z","timestamp":1653626703000},"page":"112-118","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Microscopic detection of Leptospira bacterial organisms in urine sediment from a young dog with leptospirosis and a review of the pathobiology and diagnosis of canine leptospirosis"],"prefix":"10.1111","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9548-9088","authenticated-orcid":false,"given":"Marika L.","family":"Klosowski","sequence":"first","affiliation":[{"name":"Department of Microbiology, Immunology, and Pathology College of Veterinary Medicine and Biosciences, Colorado State University  Fort Collins Colorado USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea A.","family":"Bohn","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Pathology College of Veterinary Medicine and Biosciences, Colorado State University  Fort Collins Colorado USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,5,26]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aar7975"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/jsap.12328"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2010.0654.x"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1203.050809"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-662-45059-8_5"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvsm.2010.07.008"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0007-1935(17)32291-1"},{"issue":"1","key":"e_1_2_5_9_1","first-page":"148","article-title":"Seroprevalence and risk factors associated with leptospirosis in dogs","volume":"37","author":"Meeyam T","year":"2006","journal-title":"Southeast Asian J Trop Med Public Health"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.2460\/javma.2004.225.72"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.2005.66.1816"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1354\/vp.08-VP-0265-N-REV"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph110707242"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002672"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0005915"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1086\/426455"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1159\/000187069"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-5827.1998.tb03640.x"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0940-2993(11)80185-5"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-006-0163-z"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2010.0585.x"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-1-4377-0795-3.00050-8"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-018-1547-4"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvsm.2019.02.008"},{"key":"e_1_2_5_25_1","unstructured":"SykesJ.Leptospirosis. In:GreeneCE ed.Infectious diseases of the dog and cat Elsevier\/Saunders;2012."},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.d740"}],"container-title":["Veterinary Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vcp.13129","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/vcp.13129","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vcp.13129","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,22]],"date-time":"2023-08-22T02:04:06Z","timestamp":1692669846000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vcp.13129"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,26]]},"references-count":25,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2023,3]]}},"alternative-id":["10.1111\/vcp.13129"],"URL":"https:\/\/doi.org\/10.1111\/vcp.13129","archive":["Portico"],"relation":{},"ISSN":["0275-6382","1939-165X"],"issn-type":[{"value":"0275-6382","type":"print"},{"value":"1939-165X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5,26]]},"assertion":[{"value":"2021-11-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-02-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-05-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}