{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T06:37:02Z","timestamp":1772087822058,"version":"3.50.1"},"reference-count":31,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,5,1]],"date-time":"2023-05-01T00:00:00Z","timestamp":1682899200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>There are few reports in dogs that have evaluated the utility of semi\u2010quantitative scoring of bone marrow iron stores in conjunction with reticulocyte hemoglobin (CHr) to identify iron\u2010restricted erythropoiesis due to absolute iron deficiency or iron sequestration.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>An established system for scoring iron stores in human bone marrow samples was applied to dogs. The objectives were to evaluate interobserver agreement (<jats:italic>\u039a<\/jats:italic><jats:sub>\u03c9<\/jats:sub>), determine marrow iron scores in dogs without detectable hematologic abnormalities, and assess combined interpretation of iron scores and CHr to evaluate for iron\u2010restricted erythropoiesis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Four blinded observers independently scored iron in 139 Prussian blue\u2010stained canine marrow samples from 0 (none) to 6 (very heavy), including healthy controls (n\u2009=\u200912), clinically ill dogs with (n\u2009=\u2009100) and without (n\u2009=\u200916) detectable hematologic abnormalities, and dogs with experimental nutritional iron deficiency (n\u2009=\u200911). Additional medical record data were available for 118 dogs to evaluate for other evidence of iron deficiency (abnormal CHr, RBC indices, serum iron variables, external blood loss, or nutritional deficiencies).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Mean <jats:italic>\u039a<\/jats:italic><jats:sub>\u03c9<\/jats:sub> was 0.69 (substantial agreement) for all samples but was 0.44 (moderate agreement) for samples with iron scores &lt;3, indicating distinguishing scores 0\u20132 may not be reliable. Dogs without detectable hematologic abnormalities had scores from 3\u20135. Dogs with scores &lt;3 and decreased CHr often had more indicators of iron deficiency vs dogs only having low iron scores or low CHr.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Evaluation of dogs with marrow iron score &lt;3 for external blood loss or nutritional deficiencies is likely clinically worthwhile, particularly if there is also decreased CHr.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vcp.13209","type":"journal-article","created":{"date-parts":[[2023,5,2]],"date-time":"2023-05-02T03:30:25Z","timestamp":1682998225000},"page":"243-251","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Bone marrow iron scoring in healthy and clinically ill dogs with and without evidence of iron\u2010restricted erythropoiesis"],"prefix":"10.1111","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0496-3002","authenticated-orcid":false,"given":"Grace A.","family":"Pawsat","sequence":"first","affiliation":[{"name":"Department of Biomedical and Diagnostic Sciences University of Tennessee  Knoxville Tennessee USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1744-8739","authenticated-orcid":false,"given":"Michael M.","family":"Fry","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Diagnostic Sciences University of Tennessee  Knoxville Tennessee USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9189-4238","authenticated-orcid":false,"given":"Erica","family":"Behling\u2010Kelly","sequence":"additional","affiliation":[{"name":"Department of Population Medicine and Diagnostic Sciences Cornell University  Ithaca New York USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shelly J.","family":"Olin","sequence":"additional","affiliation":[{"name":"Department of Small Animal Clinical Sciences University of Tennessee  Knoxville Tennessee USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5260-2180","authenticated-orcid":false,"given":"Deanna M. W.","family":"Schaefer","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Diagnostic Sciences University of Tennessee  Knoxville Tennessee USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,5]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"250","article-title":"Iron deficiency anemia","volume":"53","author":"Naigamwalla DZ","year":"2012","journal-title":"Can Vet J"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.3109\/10408363.2015.1038744"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2011.03495.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2018-06-856500"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.68.3.258"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.12461"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-1-4160-3661-6.00016-X"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.12294"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcpa.2015.01.008"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.12219"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V99.4.1489"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.2005.tb00004.x"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.2006.tb00110.x"},{"key":"e_1_2_7_15_1","first-page":"272","article-title":"Stability and reproducibility of ADVIA 120\u2010measured red blood cell and platelet parameters in dogs, cats, and horses, and the use of reticulocyte haemoglobin content (CH(R)) in the diagnosis of iron deficiency","volume":"134","author":"Prins M","year":"2009","journal-title":"Tijdschr Diergeneeskd"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1177\/1040638715618231"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.12499"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.13598"},{"issue":"43","key":"e_1_2_7_19_1","article-title":"Variability in the cleavage of exosome\u2010associated transferrin receptor questions the utility of clinically useful soluble transferrin receptor assays for dogs, cats, and horses","volume":"86","author":"Martinez CR","year":"2020","journal-title":"Exp Hematol"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.2009.064451"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.2003.015834"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI104793"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002770000261"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.12947"},{"key":"e_1_2_7_25_1","first-page":"511","volume-title":"Williams Hematology","author":"Beutler E","year":"2006"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.1997.tb00067.x"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-7020-4226-3.00013-5"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780470757130.ch2"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.2307\/2529310"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.12006"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3000410"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-323-06619-8.10006-4"}],"container-title":["Veterinary Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vcp.13209","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T07:53:54Z","timestamp":1698652434000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vcp.13209"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5]]},"references-count":31,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2023,6]]}},"alternative-id":["10.1111\/vcp.13209"],"URL":"https:\/\/doi.org\/10.1111\/vcp.13209","archive":["Portico"],"relation":{},"ISSN":["0275-6382","1939-165X"],"issn-type":[{"value":"0275-6382","type":"print"},{"value":"1939-165X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,5]]},"assertion":[{"value":"2022-08-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-10-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-05-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}