{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T13:28:23Z","timestamp":1789219703812,"version":"build-2803163510"},"reference-count":40,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T00:00:00Z","timestamp":1693180800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006312","name":"South African Agency for Science and Technology Advancement","doi-asserted-by":"publisher","award":["NRF CPRR160425163064"],"award-info":[{"award-number":["NRF CPRR160425163064"]}],"id":[{"id":"10.13039\/501100006312","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The regenerative response following <jats:italic>Babesia rossi<\/jats:italic> infection in dogs is mild, despite severe hemolytic anemia.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>We aimed to compare the admission absolute reticulocyte count (ARC) and reticulocyte indices in 103 dogs naturally infected with <jats:italic>B. rossi<\/jats:italic> with 10 dogs suffering from immune\u2010mediated hemolytic anemia (IMHA) and 14 healthy control dogs. The regenerative response was also evaluated in five dogs experimentally infected with <jats:italic>B. rossi<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This is a retrospective observational study of records generated on the ADVIA 2120 hematology analyzer.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The median hematocrits (HCT) of the <jats:italic>B. rossi<\/jats:italic> and IMHA groups were significantly lower than the control group (<jats:italic>p\u2009&lt;<\/jats:italic>\u2009.001 for both); however, no differences were seen between the <jats:italic>B. rossi<\/jats:italic> and IMHA groups. Compared with the control group, the median ARC was significantly higher in the <jats:italic>B. rossi<\/jats:italic> (<jats:italic>p<\/jats:italic>\u2009=\u2009.006) and IMHA (<jats:italic>p<\/jats:italic>\u2009=\u2009.019) groups but significantly lower in the <jats:italic>B. rossi<\/jats:italic> group than the IMHA group (<jats:italic>p<\/jats:italic>\u2009=\u2009.041). In the experimentally infected dogs, there was a sudden decrease in the ARC approximately 48\u2009h after the detection of peripheral parasitemia, which was followed by an increase after treatment. Reticulocytes of naturally infected <jats:italic>B. rossi<\/jats:italic> dogs were larger, with more variation in cellular volume. The reticulocytes of the experimentally infected dogs decreased in size with decreasing hemoglobin concentrations as the study progressed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The regenerative response in dogs naturally infected with <jats:italic>B. rossi<\/jats:italic> is inadequate, given the severity of the anemia observed, and it might be a result of direct suppressive action by the parasite or host response on the bone marrow.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vcp.13228","type":"journal-article","created":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T10:05:17Z","timestamp":1693217117000},"page":"422-432","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Regenerative response in dogs naturally and experimentally infected with <i>Babesia rossi<\/i>"],"prefix":"10.1111","volume":"52","author":[{"given":"Chandini","family":"Seejarim","sequence":"first","affiliation":[{"name":"Department of Companion Animal Clinical Studies, Faculty of Veterinary Science University of Pretoria  Pretoria South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9798-1874","authenticated-orcid":false,"given":"Yolandi","family":"Rautenbach","sequence":"additional","affiliation":[{"name":"Department of Companion Animal Clinical Studies, Faculty of Veterinary Science University of Pretoria  Pretoria South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4367-799X","authenticated-orcid":false,"given":"Emma H.","family":"Hooijberg","sequence":"additional","affiliation":[{"name":"Department of Companion Animal Clinical Studies, Faculty of Veterinary Science University of Pretoria  Pretoria South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew L.","family":"Leisewitz","sequence":"additional","affiliation":[{"name":"Department of Companion Animal Clinical Studies, Faculty of Veterinary Science University of Pretoria  Pretoria South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johan P.","family":"Schoeman","sequence":"additional","affiliation":[{"name":"Department of Companion Animal Clinical Studies, Faculty of Veterinary Science University of Pretoria  Pretoria South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8415-4802","authenticated-orcid":false,"given":"Amelia","family":"Goddard","sequence":"additional","affiliation":[{"name":"Department of Companion Animal Clinical Studies, Faculty of Veterinary Science University of Pretoria  Pretoria South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,8,28]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.4102\/ojvr.v76i1.66"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2011.04.023"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0195-5616(03)00099-8"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/0300985814529315"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/jsap.12498"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1080\/00034983.1998.11813308"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.4102\/jsava.v82i3.51"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2019.06.005"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.2006.tb00110.x"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.12219"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.12499"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2008.04.012"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.4102\/jsava.v74i3.512"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.15441"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.55.51"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.aap7336"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-02-0703"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2018-06-856831"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0150113"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/pim.12630"},{"issue":"4","key":"e_1_2_8_22_1","first-page":"283","article-title":"The maturation of reticulocytes. 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