{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T19:01:12Z","timestamp":1766516472896,"version":"3.40.5"},"reference-count":14,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,2,27]],"date-time":"2023-02-27T00:00:00Z","timestamp":1677456000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Toxoplasmosis is a protozoan disease caused by <jats:italic>Toxoplasma gondii<\/jats:italic>. Different <jats:italic>T. gondii<\/jats:italic> confirmatory techniques, including serologic methods, are available to detect the presence of the parasite. Among serology techniques, immunochromatographic rapid testing could be a reliable alternative to serologic laboratory techniques.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>This study evaluated a commercial immunochromatographic test (<jats:italic>FAST<\/jats:italic>est TOXOPLASMA g) in seronegative and seropositive cats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Two indirect immunofluorescence antibody reference tests, an in\u2010house technique, and a commercial test were used to classify 292 feline serum samples. The rapid test was evaluated in different groups of cats, including healthy seronegative cats (n\u00a0=\u00a0121), seropositive cats with variable anti\u2010<jats:italic>Toxoplasma<\/jats:italic> antibodies (n\u00a0=\u00a0146), and cats with positive serologic results for other pathogens (n\u00a0=\u00a025). The sensitivity, specificity, accuracy, receiver operating characteristic curves, and kappa statistics were analyzed as performance measures.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Of the 292 samples, 146 were classified as <jats:italic>T. gondii<\/jats:italic> seropositive and 146 as <jats:italic>T. gondii<\/jats:italic> seronegative. Concordant results were obtained for all samples using immunofluorescence antibody tests. The diagnostic measures of this rapid test showed 98.63% sensitivity and 100% specificity, and 99.32% accuracy. The kappa statistics value was 0.986, and the area under the receiver operating characteristic curve was 0.993.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This rapid test showed diagnostic measurements similar to those of traditional quantitative serologic methods. In situations where laboratory techniques are not available, this test, under clinical conditions, could be a useful alternative to obtain accurate results rapidly.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vcp.13230","type":"journal-article","created":{"date-parts":[[2023,2,28]],"date-time":"2023-02-28T03:08:02Z","timestamp":1677553682000},"page":"284-287","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Evaluation of an immunochromatographic serologic test to detect the presence of anti\u2010<i>Toxoplasma gondii<\/i> antibodies in cats"],"prefix":"10.1111","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6209-4282","authenticated-orcid":false,"given":"Sergio","family":"Villanueva\u2010Saz","sequence":"first","affiliation":[{"name":"Department of Animal Pathology, Veterinary Faculty University of Zaragoza  Zaragoza Spain"},{"name":"Clinical Immunology Laboratory, Veterinary Faculty University of Zaragoza  Zaragoza Spain"},{"name":"Instituto Agroalimentario de Arag\u00f3n\u2010IA2 (University of Zaragoza\u2010CITA)  Zaragoza Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariv\u00ed","family":"Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Department of Animal Pathology, Veterinary Faculty University of Zaragoza  Zaragoza Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacobo","family":"Giner","sequence":"additional","affiliation":[{"name":"Department of Animal Pathology, Veterinary Faculty University of Zaragoza  Zaragoza Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Dolores","family":"P\u00e9rez","sequence":"additional","affiliation":[{"name":"Instituto Agroalimentario de Arag\u00f3n\u2010IA2 (University of Zaragoza\u2010CITA)  Zaragoza Spain"},{"name":"Department of Animal Production and Sciences of the Food, Veterinary Faculty University of Zaragoza  Zaragoza Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Pilar","family":"Tobajas","sequence":"additional","affiliation":[{"name":"Instituto Agroalimentario de Arag\u00f3n\u2010IA2 (University of Zaragoza\u2010CITA)  Zaragoza Spain"},{"name":"Department of Animal Production and Sciences of the Food, Veterinary Faculty University of Zaragoza  Zaragoza Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e9s","family":"Yzuel","sequence":"additional","affiliation":[{"name":"Clinical Immunology Laboratory, Veterinary Faculty University of Zaragoza  Zaragoza Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Teresa","family":"Verde","sequence":"additional","affiliation":[{"name":"Department of Animal Pathology, Veterinary Faculty University of Zaragoza  Zaragoza Spain"},{"name":"Clinical Immunology Laboratory, Veterinary Faculty University of Zaragoza  Zaragoza Spain"},{"name":"Instituto Agroalimentario de Arag\u00f3n\u2010IA2 (University of Zaragoza\u2010CITA)  Zaragoza Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Delia","family":"Lacasta","sequence":"additional","affiliation":[{"name":"Department of Animal Pathology, Veterinary Faculty University of Zaragoza  Zaragoza Spain"},{"name":"Instituto Agroalimentario de Arag\u00f3n\u2010IA2 (University of Zaragoza\u2010CITA)  Zaragoza Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Department of Animal Pathology, Veterinary Faculty University of Zaragoza  Zaragoza Spain"},{"name":"Clinical Immunology Laboratory, Veterinary Faculty University of Zaragoza  Zaragoza Spain"},{"name":"Instituto Agroalimentario de Arag\u00f3n\u2010IA2 (University of Zaragoza\u2010CITA)  Zaragoza Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diana","family":"Marteles","sequence":"additional","affiliation":[{"name":"Department of Animal Pathology, Veterinary Faculty University of Zaragoza  Zaragoza Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H\u00e9ctor","family":"Ru\u00edz","sequence":"additional","affiliation":[{"name":"Department of Animal Pathology, Veterinary Faculty University of Zaragoza  Zaragoza Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,2,27]]},"reference":[{"key":"e_1_2_3_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/1098612X13489228"},{"key":"e_1_2_3_3_1","first-page":"625","article-title":"The effect of glucocorticoid administration on oocyst shedding, serology, and cell mediated immune responses of cats with recent or chronic toxoplasmosis","volume":"27","author":"Lappin MR","year":"1992","journal-title":"J Am Anim Hosp Assoc"},{"key":"e_1_2_3_4_1","doi-asserted-by":"publisher","DOI":"10.1590\/s1984-29612014078"},{"key":"e_1_2_3_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0195-5616(00)05006-3"},{"key":"e_1_2_3_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2020.109145"},{"key":"e_1_2_3_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-009-1358-4"},{"key":"e_1_2_3_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2015.04.035"},{"key":"e_1_2_3_9_1","doi-asserted-by":"publisher","DOI":"10.1590\/S1984-29612012000200007"},{"key":"e_1_2_3_10_1","first-page":"1","article-title":"A cross\u2010sectional serosurvey of SARS\u2010CoV\u20102 and co\u2010infections in stray cats from the second wave to the sixth wave of COVID\u201019 outbreaks in Spain","volume":"14","author":"Villanueva\u2010Saz S","year":"2022","journal-title":"Vet Res Commun"},{"issue":"90","key":"e_1_2_3_11_1","first-page":"E5","article-title":"Molecular epidemiology of Bartonella henselae in stray and sheltered cats of Zaragoza, Spain","volume":"2016","author":"Alam\u00e1n Valtierra M","year":"2016","journal-title":"Rev Esp Salud Publica"},{"key":"e_1_2_3_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.12408"},{"key":"e_1_2_3_13_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.105.6.557"},{"key":"e_1_2_3_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-7-111"},{"key":"e_1_2_3_15_1","doi-asserted-by":"publisher","DOI":"10.3347\/kjp.2011.49.3.207"}],"container-title":["Veterinary Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vcp.13230","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/vcp.13230","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vcp.13230","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T07:54:59Z","timestamp":1698652499000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vcp.13230"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2,27]]},"references-count":14,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2023,6]]}},"alternative-id":["10.1111\/vcp.13230"],"URL":"https:\/\/doi.org\/10.1111\/vcp.13230","archive":["Portico"],"relation":{},"ISSN":["0275-6382","1939-165X"],"issn-type":[{"type":"print","value":"0275-6382"},{"type":"electronic","value":"1939-165X"}],"subject":[],"published":{"date-parts":[[2023,2,27]]},"assertion":[{"value":"2022-05-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-12-21","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-02-27","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}