{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:23:56Z","timestamp":1787016236369,"version":"build-2736575974"},"reference-count":20,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T00:00:00Z","timestamp":1690848000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2023,12]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n Distinguishing primary and secondary pulmonary neoplasms can be challenging via cytology, and a rapid, inexpensive diagnostic tool to differentiate these neoplasms is unavailable. Alkaline phosphatase cytochemistry (ALP\u2010CC) has been used to identify primary pulmonary carcinomas in human patients, and we hypothesized it could be applied to canine lung aspirates.<\/jats:p>\n <\/jats:sec>\n \n Objective<\/jats:title>\n We aimed to characterize ALP\u2010CC expression in fine\u2010needle aspirate (FNA) samples of canine pulmonary neoplastic and non\u2010neoplastic tumors.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n A retrospective case search was conducted to identify cases with contemporaneous cytology and histopathology reports from pulmonary lesions, including neoplastic and non\u2010neoplastic etiologies. Slides prepared from pulmonary aspirates were stained for ALP\u2010CC activity, and the percentage of ALP\u2010CC\u2010positive primary pulmonary epithelial tumors was determined. To characterize the ALP\u2010CC expression in non\u2010neoplastic cellular constituents of pulmonary FNA samples, mesothelial cells were also evaluated.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n Forty\u2010eight canine cases met the inclusion criteria. ALP\u2010CC\u2013positive cells were seen in both neoplastic and non\u2010neoplastic lesions. In non\u2010neoplastic lesions, pulmonary epithelial cells were ALP\u2010CC positive. Eighty\u2010nine percent of primary pulmonary epithelial neoplasms were ALP\u2010CC positive, and no ALP\u2010CC positivity was noted in mesothelial cells. ALP\u2010CC\u2013positive neoplastic cells were seen in a metastatic amelanotic melanoma.<\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n Primary pulmonary epithelial neoplasms are frequently ALP\u2010CC positive, but such positivity is not restricted to this tumor type. Non\u2010neoplastic pulmonary epithelial cells can be ALP\u2010CC positive, whereas mesothelial cells are negative.<\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/vcp.13250","type":"journal-article","created":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T22:41:20Z","timestamp":1690929680000},"page":"661-669","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Characterization of alkaline phosphatase cytochemistry in canine neoplastic and non\u2010neoplastic pulmonary mass aspirates"],"prefix":"10.1111","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1964-3087","authenticated-orcid":false,"given":"Jack","family":"O'Day","sequence":"first","affiliation":[{"name":"Department of Veterinary Clinical Sciences, College of Veterinary Medicine University of Minnesota St. Paul Minnesota 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