{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T21:06:18Z","timestamp":1773954378199,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,11,1]],"date-time":"2023-11-01T00:00:00Z","timestamp":1698796800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009945","name":"Funda\u00e7\u00e3o de Apoio \u00e0 Pesquisa, Universidade Federal de Goi\u00e1s","doi-asserted-by":"publisher","award":["06\/2018"],"award-info":[{"award-number":["06\/2018"]}],"id":[{"id":"10.13039\/501100009945","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Cholinesterase is a biomarker for poisonings by anticholinesterase agents, but its reference values are scarce, and possible interaction with collars containing parasiticides has not been studied.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>We aimed to evaluate the serum cholinesterase activity of healthy dogs without a history of contact with anticholinesterase agents and healthy animals exposed to commercial collars containing organophosphate.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Ninety\u2010nine dogs were used and included healthy animals without recent exposure to anticholinesterase agents and healthy animals previously exposed to diazinon collars. Serum quantification of the enzyme butyrylcholinesterase (BuchE) through spectrophotometry was conducted on all samples. In experiment 1, BuchE activity was quantified at time 0 and 7\u2009days after, a time when the samples were kept at \u221218\u00b0C. In experiment 2, sampling times were 0, 7, 14, 21, 28, and 56\u2009days.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Time 0 values were 4622.38\u2009\u00b1\u20091311.53\u2009U\/L. After 7\u2009days, a significant decay was observed, with a mean of 3934.45\u2009\u00b1\u20091430.45\u2009U\/L. Spearman's test was performed, finding a weak correlation between ALT, creatinine, total plasma proteins, age, weight, red blood cells, platelets, leukocytes, and BuchE activities. In experiment 2, the mean at time 0 was 4753\u2009\u00b1\u2009454.8\u2009U\/L. With exposure to the collar, there was a decay of up to 93% after 14\u2009days.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Normality values of serum BuchE in healthy dogs without a history of exposure to anticholinesterase agents were 4360.8\u20134883.96\u2009U\/L. Freezing serum caused a decrease in BuchE activity. Exposure to commercial collars containing diazinon also reduced BuchE activity without clinical signs, indicating that previously exposed animals should be evaluated carefully.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vcp.13298","type":"journal-article","created":{"date-parts":[[2023,11,2]],"date-time":"2023-11-02T02:45:20Z","timestamp":1698893120000},"page":"646-653","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Serum butyrylcholinesterase activity in healthy dogs with and without exposure to diazinon"],"prefix":"10.1111","volume":"52","author":[{"given":"Sara S. F.","family":"Almeida","sequence":"first","affiliation":[{"name":"Laboratory of Veterinary Toxicology, Veterinary Hospital, Department of Veterinary Medicine, School of Veterinary and Animal Science Goi\u00e1s Federal University  Goi\u00e1s Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rayanne H. 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M.","family":"Barros","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Toxicology, Veterinary Hospital, Department of Veterinary Medicine, School of Veterinary and Animal Science Goi\u00e1s Federal University  Goi\u00e1s Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monica C.","family":"Vicente","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Toxicology, Veterinary Hospital, Department of Veterinary Medicine, School of Veterinary and Animal Science Goi\u00e1s Federal University  Goi\u00e1s Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00edlia M.","family":"Melo","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinic and Surgery, Veterinary School Federal University of Minas Gerais  Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naida C.","family":"Borges","sequence":"additional","affiliation":[{"name":"Radiology and Ultrasonography, Clinic and Surgery Sector, Veterinary Hospital, Department of Veterinary Medicine. School of Veterinary and Animal Science Goi\u00e1s Federal University  Goi\u00e1s Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danieli B.","family":"Martins","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Clinical Pathology, Clinic and Surgery Sector, Veterinary Hospital, Department of Veterinary Medicine, School of Veterinary and Animal Science Goi\u00e1s Federal University  Goi\u00e1s Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5204-0512","authenticated-orcid":false,"given":"Ana F. M.","family":"Botelho","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary Toxicology, Veterinary Hospital, Department of Veterinary Medicine, School of Veterinary and Animal Science Goi\u00e1s Federal University  Goi\u00e1s Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,11]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvsm.2018.06.005"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2018.01.044"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.emc.2014.09.010"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11356-018-2465-5"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0103-84782010000500017"},{"key":"e_1_2_7_7_1","first-page":"739","article-title":"Intoxica\u00e7\u00e3o por Organofosforados: Tratamento e Metodologias Anal\u00edticas Empregadas na Avalia\u00e7\u00e3o da Reativa\u00e7\u00e3o e Inibi\u00e7\u00e3o da Acetilcolinesterase. 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