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Physical examination revealed a large, firm mass extending from the humerus to the distal aspect of the elbow. Computed tomography confirmed a large aggressive mass of the right distal humerus with a large soft tissue component, severe osteolysis, and adjacent periosteal proliferation. Fine\u2010needle aspirates of the mass were most compatible with sarcoma, and osteosarcoma was prioritized. An unstained slide was treated with nitroblue tetrazolium chloride\/5\u2010bromo\u20104\u2010chloro\u20103\u2010indolyl phosphate toluidine salt\u2010phosphatase (NBT\/BCIP) substrate for ALP detection and was strongly positive, confirming a diagnosis of osteosarcoma. A month later, the patient underwent wing amputation and arrested during recovery from anesthesia. Post\u2010mortem examination and histopathology were consistent with osteosarcoma. This case report highlights a rare occurrence of osteosarcoma in a cockatoo as well as its cytologic and histologic features. Additionally, this report provides support for NBT\/BCIP application in ALP\u2010expressing tumors, a cytochemical stain that has been minimally investigated in avian species.<\/jats:p>","DOI":"10.1111\/vcp.13322","type":"journal-article","created":{"date-parts":[[2024,1,2]],"date-time":"2024-01-02T10:35:41Z","timestamp":1704191741000},"page":"111-115","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Osteosarcoma of the wing in a sulfur\u2010crested cockatoo"],"prefix":"10.1111","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-4755-2907","authenticated-orcid":false,"given":"Sydney A.","family":"Hastain","sequence":"first","affiliation":[{"name":"Department of Population Health and Pathobiology, College of Veterinary Medicine North Carolina State University  Raleigh North Carolina 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