{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T03:47:43Z","timestamp":1775965663762,"version":"3.50.1"},"reference-count":46,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,3,3]],"date-time":"2024-03-03T00:00:00Z","timestamp":1709424000000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Immune thrombocytopenia (ITP) is commonly associated with platelet\u2010associated immunoglobulins (PAIg). Demonstration of PAIg can help determine etiologies for thrombocytopenia. In humans, ITP and thrombocytopenia have been associated with various vaccinations and influenza infections, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>We aimed to evaluate platelet counts and PAIg in research dogs with H3N2 and in research and client\u2010owned dogs routinely vaccinated for distemper, adenovirus\u20102, parainfluenza, and parvovirus (DA2PP). The hypotheses were that H3N2 infection but not DA2PP vaccination would decrease platelet counts, and neither would result in the detection of PAIg.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Three pilot studies. Platelet counts and PAIg, measured by direct flow cytometry as %IgG, were evaluated in eight research Beagles following experimental infection with H3N2 (experiment 1), nine research Beagles vaccinated for DA2PP (experiment 2), and thirty client\u2010owned dogs vaccinated for DA2PP (experiment 3). All animals were considered healthy at the start of the experiments.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Transient, self\u2010resolving decreases in platelet counts and increases in %IgG occurred following H3N2 infection, and one dog became thrombocytopenic and positive for PAIg. Following DA2PP vaccination, %IgG increased in research and client\u2010owned dogs, but only one dog was considered positive for PAIg with a concurrent increase in platelet count. Mean PAIg increased from baseline in client\u2010owned dogs following vaccination.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Transient PAIg and thrombocytopenia can occur following H3N2 infection, while routine vaccination for DA2PP in this group of dogs was not associated with the development of thrombocytopenia or clinically relevant formation of PAIg.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vcp.13333","type":"journal-article","created":{"date-parts":[[2024,3,4]],"date-time":"2024-03-04T03:29:18Z","timestamp":1709522958000},"page":"47-56","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Effects of canine influenza infection and <scp>DA2PP<\/scp> vaccination on the development of platelet\u2010associated immunoglobulins and platelet counts in dogs"],"prefix":"10.1111","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1277-3946","authenticated-orcid":false,"given":"Maggie","family":"Williams","sequence":"first","affiliation":[{"name":"Department of Clinical Sciences, Center for Companion Animal Studies Colorado State University  Fort Collins Colorado USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6472-5452","authenticated-orcid":false,"given":"Sarah","family":"Shropshire","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, Center for Companion Animal Studies Colorado State University  Fort Collins Colorado USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nida","family":"Chornarm","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, Center for Companion Animal Studies Colorado State University  Fort Collins Colorado 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