{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T02:54:41Z","timestamp":1775530481052,"version":"3.50.1"},"reference-count":12,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,2,23]],"date-time":"2024-02-23T00:00:00Z","timestamp":1708646400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Although widely used, the ADVIA 120 hematology analyzer has not been previously validated for determining the differential leukocyte count in goats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>The aim of this study was to compare the differential leukocyte counts provided by the ADVIA 120 (A\u2010diff) and the manual method (M\u2010Diff) in goats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>EDTA blood samples that were analyzed within 4\u2009h of collection were used in the study. The following exclusion criteria were applied: inappropriately filled tubes or tubes containing clots, erroneous ADVIA peroxidase cytograms, and blood smears of poor quality. The A\u2010Diff was compared with the M\u2010Diff performed by two independent observers on 200 leukocytes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Forty samples were included after previously excluding eight samples. The correlation between the A\u2010Diff and M\u2010Diff was very strong for eosinophils (<jats:italic>r<\/jats:italic>\u2009=\u2009.870, <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.001) and strong for lymphocytes (<jats:italic>r<\/jats:italic>\u2009=\u2009.796, <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.001) and neutrophils (<jats:italic>r<\/jats:italic>\u2009=\u2009.730, <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.001), while no significant correlation was observed for monocytes (<jats:italic>r<\/jats:italic>\u2009=\u2009.026, <jats:italic>p<\/jats:italic>\u2009=\u2009.872). The Passing\u2013Bablok regression analyses revealed statistically significant constant errors for neutrophils (5.83%; 95% confidence interval [CI]: 0.41%, 12.18%) and eosinophils (1.89%; 95% CI: 1.17%, 2.71%). Bland\u2013Altman analyses showed a statistically significant negative bias for lymphocytes (\u22125.0%) and a statistically significant positive bias for eosinophils (2.2%). The very low basophil percentages precluded a meaningful method comparison.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The ADVIA 120 overall demonstrated good performance for the differential WBC count in goats under the conditions of this study. Therefore, it can be considered suitable for routine hematologic screening in goats. Nonetheless, it should be emphasized that any abnormal result should be confirmed with a blood smear evaluation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vcp.13337","type":"journal-article","created":{"date-parts":[[2024,2,24]],"date-time":"2024-02-24T01:14:10Z","timestamp":1708737250000},"page":"57-62","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["A comparison study between the Siemens ADVIA 120 and manual method for the differential white blood cell count in goats"],"prefix":"10.1111","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1591-9891","authenticated-orcid":false,"given":"Ioannis L.","family":"Oikonomidis","sequence":"first","affiliation":[{"name":"Department of Veterinary Anatomy, Physiology and Pathology, Institute of Infection, Veterinary and Ecological Studies University of Liverpool  Liverpool UK"},{"name":"Diagnostic Laboratory, School of Veterinary Medicine, Faculty of Health Sciences Aristotle University of Thessaloniki  Thessaloniki Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christos","family":"Brozos","sequence":"additional","affiliation":[{"name":"Farm Animal Clinic, School of Veterinary Medicine, Faculty of Health Sciences Aristotle University of Thessaloniki  Thessaloniki Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4032-0338","authenticated-orcid":false,"given":"Theodora K.","family":"Tsouloufi","sequence":"additional","affiliation":[{"name":"Diagnostic Laboratory, School of Veterinary Medicine, Faculty of Health Sciences Aristotle University of Thessaloniki  Thessaloniki Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Evangelos","family":"Kiossis","sequence":"additional","affiliation":[{"name":"Farm Animal Clinic, School of Veterinary Medicine, Faculty of Health Sciences Aristotle University of Thessaloniki  Thessaloniki Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Kritsepi\u2010Konstantinou","sequence":"additional","affiliation":[{"name":"Diagnostic Laboratory, School of Veterinary Medicine, Faculty of Health Sciences Aristotle University of Thessaloniki  Thessaloniki Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,2,23]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvfa.2007.07.002"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0272-2712(03)00075-1"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.12935"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-1-4377-0173-9.00002-6"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.12810"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.12814"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.2006.tb00128.x"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00769-007-0323-0"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.2008.00084.x"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.2009.00126.x"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165X.1996.tb00960.x"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.12558"}],"container-title":["Veterinary Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vcp.13337","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,3,29]],"date-time":"2024-03-29T10:48:51Z","timestamp":1711709331000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vcp.13337"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2,23]]},"references-count":12,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,3]]}},"alternative-id":["10.1111\/vcp.13337"],"URL":"https:\/\/doi.org\/10.1111\/vcp.13337","archive":["Portico"],"relation":{},"ISSN":["0275-6382","1939-165X"],"issn-type":[{"value":"0275-6382","type":"print"},{"value":"1939-165X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,2,23]]},"assertion":[{"value":"2023-04-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-01-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}