{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,27]],"date-time":"2025-08-27T16:36:26Z","timestamp":1756312586886,"version":"3.40.5"},"reference-count":14,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,6,25]],"date-time":"2024-06-25T00:00:00Z","timestamp":1719273600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A 7\u2010year\u2010old female spayed Bernese Mountain dog was presented for evaluation of hematuria. Incidentally, a right stifle sarcoma was diagnosed via cytology, which raised concern for histiocytic sarcoma (given the patient's signalment) versus another joint\u2010associated sarcoma. Histopathology and immunohistochemistry revealed a CD18\u2010negative, non\u2010histiocytic origin cell population. Findings were consistent with a joint\u2010associated grade II soft tissue sarcoma (STS). The patient's hematuria was progressive over 5\u2009months, and urinary bladder transitional cell carcinoma (TCC) was diagnosed via cystoscopy and histopathology. An enlarged right medial iliac lymph node was identified on routine restaging via abdominal ultrasound 3\u2009months later. Cytology of the lymph node revealed a markedly pleomorphic cell population, again raising concern for histiocytic sarcoma (HS). Other differentials included an anaplastic metastatic population from the joint\u2010associated STS or the TCC. Immunocytochemistry revealed a cytokeratin\u2010positive, CD18\u2010, CD204\u2010, and vimentin\u2010negative cell population, consistent with a carcinoma. DNA was extracted from cytology slides to sequence cells for BRAF mutation status. Sequencing revealed a homozygous V596E (transcript ENSCAFT00845055173.1) BRAF mutation, consistent with the known biology of TCC. In neither case was HS truly present in this patient, but immunocytochemistry provided information that helped to optimize the patient's chemotherapy recommendations.<\/jats:p>","DOI":"10.1111\/vcp.13360","type":"journal-article","created":{"date-parts":[[2024,6,26]],"date-time":"2024-06-26T02:50:15Z","timestamp":1719370215000},"page":"309-314","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["It's not always histiocytic sarcoma: Immunocytochemistry to identify two unusual tumors in a Bernese Mountain dog"],"prefix":"10.1111","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-1063-043X","authenticated-orcid":false,"given":"Claire","family":"Quinn","sequence":"first","affiliation":[{"name":"Wheat Ridge Animal Hospital  Wheat Ridge Colorado USA"},{"name":"Colorado State University  Fort Collins Colorado USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kayla","family":"Harding","sequence":"additional","affiliation":[{"name":"Wheat Ridge Animal Hospital  Wheat Ridge Colorado USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6117-0909","authenticated-orcid":false,"given":"Lisa J.","family":"Schlein","sequence":"additional","affiliation":[{"name":"Colorado State University  Fort Collins Colorado USA"},{"name":"Zoetis Reference Laboratories  Wheat Ridge Colorado USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9344-6639","authenticated-orcid":false,"given":"J\u00e9r\u00e9mie","family":"Korchia","sequence":"additional","affiliation":[{"name":"Colorado State University  Fort Collins Colorado USA"},{"name":"Michigan State University  Lansing Michigan USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura L.","family":"Coffee","sequence":"additional","affiliation":[{"name":"Zoetis Reference Laboratories  Wheat Ridge Colorado USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1690-3787","authenticated-orcid":false,"given":"Amy L.","family":"MacNeill","sequence":"additional","affiliation":[{"name":"Colorado State University  Fort Collins Colorado USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katherine","family":"Tucker\u2010Mohl","sequence":"additional","affiliation":[{"name":"Diagnostic Imaging for Animals  Wheat Ridge Colorado USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5310-0078","authenticated-orcid":false,"given":"Dawn L.","family":"Duval","sequence":"additional","affiliation":[{"name":"Colorado State University  Fort Collins Colorado USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura 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