{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T19:49:51Z","timestamp":1792612191718,"version":"4.5.0"},"reference-count":17,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,10,21]],"date-time":"2024-10-21T00:00:00Z","timestamp":1729468800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,10,21]],"date-time":"2024-10-21T00:00:00Z","timestamp":1729468800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Urinary extracellular vesicles (UEVs) are membranous particles that carry renal tubular transporter proteins. Here, we evaluate whether selected renal tubular transporter proteins can be detected in UEVs isolated from small volume (1\u20135\u2009mL) canine urine samples of healthy dogs and canine patients with elevated circulating parathyroid hormone (PTH)\/PTH\u2010related peptide (PTHrp) concentrations, hypercortisolism, and primary hypoadrenocorticism using liquid chromatography\u2013tandem mass spectrometry (LC\u2013MS\/MS). The total creatinine content of each urine sample was calculated from urine volume and creatinine concentration. UEVs were isolated by size exclusion chromatography prior to quantification by nanoparticle tracking analysis and proteomic analysis by LC\u2013MS\/MS. Group comparisons were made using non\u2010parametric statistics. Aquaporin\u20102 (AQP2) and the renal sodium\/phosphate co\u2010transporter (NPT2A) were detected in UEVs isolated from small volume samples of almost all healthy dogs but were not detected in most dogs with elevated circulating PTH\/PTH related peptide (PTHrp) concentrations, hypercortisolism and primary hypoadrenocorticism. Total creatinine content of the urine sample was strongly positively correlated with the number of UEVs (\n                    <jats:italic>r<\/jats:italic>\n                    <jats:sub>s<\/jats:sub>\n                    \u2009=\u2009.84,\n                    <jats:italic>P<\/jats:italic>\n                    \u2009&lt;\u2009.001); thus, total creatinine was used as a surrogate marker of UEV number. In healthy dogs, AQP2 and NPT2A were both detected in samples containing at least 1.7\u2009\u00d7\u200910\n                    <jats:sup>9<\/jats:sup>\n                    UEVs or 24\u2009\u03bcmol creatinine, however in non\u2010healthy dogs, AQP2 and NPT2A were not detected in most samples containing up to 6.3\u2009\u00d7\u200910\n                    <jats:sup>9<\/jats:sup>\n                    UEVs or 32\u2009\u03bcmol creatinine.\n                  <\/jats:p>","DOI":"10.1111\/vcp.13393","type":"journal-article","created":{"date-parts":[[2024,10,21]],"date-time":"2024-10-21T11:00:33Z","timestamp":1729508433000},"page":"172-177","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Detection of renal tubular transporter proteins in canine urinary extracellular vesicles using liquid chromatography\u2013tandem mass spectrometry"],"prefix":"10.1111","volume":"55","author":[{"given":"L. J.","family":"McGravey","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine University of Cambridge  Cambridge UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. R.","family":"Antrobus","sequence":"additional","affiliation":[{"name":"Cambridge Institute for Medical Research, University of Cambridge  Cambridge UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9775-8285","authenticated-orcid":false,"given":"T. L.","family":"Williams","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine University of Cambridge  Cambridge UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,10,21]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jev2.12304"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jev2.12181"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.ajkd.2007.07.022"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.V1071416"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40620-019-00653-8"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfu362"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.1987.252.4.R635"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.V9122181"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ki.5000148"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI7840"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1998.629bm.x"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/jsap.13536"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22094881"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201100477"},{"issue":"10","key":"e_1_2_7_16_1","doi-asserted-by":"crossref","first-page":"2116","DOI":"10.2460\/ajvr.1985.46.10.2116","article-title":"Estimation of quantitative proteinuria in the dog, using the urine protein\u2010to\u2010creatinine ratio from a random, voided sample","volume":"46","author":"Grauer GF","year":"1985","journal-title":"Am J Vet Res"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2012.00915.x"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.21-0325"}],"container-title":["Veterinary Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vcp.13393","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/vcp.13393","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vcp.13393","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T19:35:21Z","timestamp":1792611321000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vcp.13393"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,21]]},"references-count":17,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1111\/vcp.13393"],"URL":"https:\/\/doi.org\/10.1111\/vcp.13393","archive":["Portico"],"relation":{},"ISSN":["0275-6382","1939-165X"],"issn-type":[{"value":"0275-6382","type":"print"},{"value":"1939-165X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,21]]},"assertion":[{"value":"2024-02-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-09-04","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-21","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}