{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T06:54:07Z","timestamp":1770965647451,"version":"3.50.1"},"reference-count":181,"publisher":"Wiley","issue":"S2","license":[{"start":{"date-parts":[[2025,1,26]],"date-time":"2025-01-26T00:00:00Z","timestamp":1737849600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2025,1,26]],"date-time":"2025-01-26T00:00:00Z","timestamp":1737849600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>MicroRNAs (miRNAs or miRs) are small, non\u2010coding RNAs that play a crucial role in gene regulation, making them potential biomarkers for various diseases. In the field of veterinary medicine, there is a growing interest in exploring the diagnostic and therapeutic potential of miRNAs in kidney diseases affecting dogs and cats. This review focuses on the use of urinary miRNAs as biomarkers for chronic kidney disease (CKD) in these companion animals. We introduce miRNAs, their biogenesis, and their presence in biofluids, particularly within exosomes, and discuss studies investigating miRNAs in kidney tissue and urine. We acknowledge the challenges associated with miRNA studies, including preanalytical factors such as biological variation, sample collection\/processing, storage conditions, and experimental design. We highlight the importance of technical considerations, such as sample pooling, sequencing depth, multiplexing, and the various steps of the miRNA experimental workflow. Furthermore, we discuss RNA isolation methods, small RNA sequencing data analysis, and the use of quantitative reverse transcription PCR (qRT\u2010PCR) and droplet digital PCR for verification. We emphasize the importance of internal controls, spike\u2010ins, and normalization methods to minimize technical variation and ensure reliable results in qRT\u2010PCR analysis. This review concludes that while urinary miRNAs hold promise as non\u2010invasive biomarkers for CKD in dogs and cats, addressing the challenges and standardization of protocols is vital for the successful translation of this research into clinical practice.<\/jats:p>","DOI":"10.1111\/vcp.13412","type":"journal-article","created":{"date-parts":[[2025,1,26]],"date-time":"2025-01-26T23:48:06Z","timestamp":1737935286000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Technical considerations and review of urinary microRNAs as biomarkers for chronic kidney disease in dogs and cats"],"prefix":"10.1111","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8543-7233","authenticated-orcid":false,"given":"Candice P.","family":"Chu","sequence":"first","affiliation":[{"name":"Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences Texas A&amp;M University  College Station Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary B.","family":"Nabity","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences Texas A&amp;M University  College Station Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,1,26]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature02871"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.01.002"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3838"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2010.147405"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0003694"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrfmmm.2011.03.004"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1596"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.3402\/jev.v2i0.22859"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/ki.2010.106"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2404132"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks658"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1408301111"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature04367"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature04138"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2634"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg3074"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrneph.2014.202"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-016-2958-x"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2014.01.003"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-96870-y"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1177\/0300985818806050"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/ki.2011.345"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/ki.2013.502"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0013515"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-13-357"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1261\/rna.1851510"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2843"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2012.05.036"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2015.04.036"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/cpr.12857"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq601"},{"key":"e_1_2_7_33_1","first-page":"3","volume-title":"Sorting It Out: regulation of Exosome Loading","author":"Villarroya\u2010Beltri C","year":"2014"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/ki.2012.256"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.22226"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00148.2013"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfv128"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrurol.2016.193"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.euf.2015.11.006"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.8124"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-016-0523-5"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2013.09.027"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep40601"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/ki.2010.278"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1186\/1476-4598-13-256"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.1129"},{"key":"e_1_2_7_47_1","unstructured":"PubMed.Pubmed Search Query \u2018((dog OR canine) OR (cat OR feline)) AND (microRNA OR miRNA) AND (urine OR urinary) AND (kidney OR renal OR nephropathy)\u2019.https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=(dog+OR+canine)+OR+(cat+OR+feline)+AND+(microRNA+OR+miRNA)+AND+(urine+OR+urinary)+AND+(kidney+OR+renal+OR+nephropathy). Accessed October 2 2024."},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep40340"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2018.00289"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.15628"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0270067"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.12743"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.16932"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.12206"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13048-015-0208-5"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-016-3168-2"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2014.01.002"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2017.06.001"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0158160"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-016-2529-1"},{"key":"e_1_2_7_61_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0174892"},{"key":"e_1_2_7_62_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2011.213363"},{"key":"e_1_2_7_63_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00711.2013"},{"key":"e_1_2_7_64_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0057496"},{"key":"e_1_2_7_65_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12263-015-0483-1"},{"key":"e_1_2_7_66_1","doi-asserted-by":"publisher","DOI":"10.3402\/jev.v5.30281"},{"key":"e_1_2_7_67_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ki.5000273"},{"key":"e_1_2_7_68_1","doi-asserted-by":"publisher","DOI":"10.3402\/jev.v2i0.20360"},{"key":"e_1_2_7_69_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-9833-3_3"},{"key":"e_1_2_7_70_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41581-022-00608-6"},{"key":"e_1_2_7_71_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0058014"},{"key":"e_1_2_7_72_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep27209"},{"key":"e_1_2_7_73_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-47504-x"},{"key":"e_1_2_7_74_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13277-011-0154-9"},{"key":"e_1_2_7_75_1","doi-asserted-by":"publisher","DOI":"10.1002\/mc.20712"},{"key":"e_1_2_7_76_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jns.2015.06.019"},{"key":"e_1_2_7_77_1","doi-asserted-by":"publisher","DOI":"10.1177\/030098587701400101"},{"key":"e_1_2_7_78_1","doi-asserted-by":"publisher","DOI":"10.1177\/030098587701400204"},{"key":"e_1_2_7_79_1","doi-asserted-by":"publisher","DOI":"10.1177\/1098612X13511446"},{"key":"e_1_2_7_80_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2009.0339.x"},{"key":"e_1_2_7_81_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/7932765"},{"key":"e_1_2_7_82_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdiacomp.2016.07.012"},{"key":"e_1_2_7_83_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejps.2016.10.008"},{"key":"e_1_2_7_84_1","doi-asserted-by":"publisher","DOI":"10.1002\/pros.23295"},{"key":"e_1_2_7_85_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2015.12.011"},{"key":"e_1_2_7_86_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00569.2016"},{"key":"e_1_2_7_87_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0150154"},{"key":"e_1_2_7_88_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.3358"},{"key":"e_1_2_7_89_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urolonc.2009.01.027"},{"key":"e_1_2_7_90_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0073798"},{"key":"e_1_2_7_91_1","volume-title":"Best practices: recommendations for repositories","author":"Snapes E","year":"2023"},{"key":"e_1_2_7_92_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00511a030"},{"key":"e_1_2_7_93_1","doi-asserted-by":"publisher","DOI":"10.1515\/cclm-2013-0562"},{"key":"e_1_2_7_94_1","doi-asserted-by":"publisher","DOI":"10.2217\/bmm.13.44"},{"key":"e_1_2_7_95_1","doi-asserted-by":"publisher","DOI":"10.1002\/jev2.12404"},{"key":"e_1_2_7_96_1","doi-asserted-by":"publisher","DOI":"10.3390\/life12030386"},{"key":"e_1_2_7_97_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw545"},{"key":"e_1_2_7_98_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471142905.hg1113s83"},{"key":"e_1_2_7_99_1","doi-asserted-by":"publisher","DOI":"10.1261\/rna.053959.115"},{"key":"e_1_2_7_100_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagrm.2008.03.012"},{"key":"e_1_2_7_101_1","doi-asserted-by":"publisher","DOI":"10.3402\/jev.v2i0.19861"},{"key":"e_1_2_7_102_1","doi-asserted-by":"publisher","DOI":"10.3402\/jev.v5.32945"},{"key":"e_1_2_7_103_1","doi-asserted-by":"publisher","DOI":"10.3402\/jev.v4.26533"},{"key":"e_1_2_7_104_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2024.101269"},{"key":"e_1_2_7_105_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-60761-711-2_6"},{"key":"e_1_2_7_106_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00434.2006"},{"key":"e_1_2_7_107_1","doi-asserted-by":"publisher","DOI":"10.1111\/jsap.13536"},{"key":"e_1_2_7_108_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2014.221341"},{"key":"e_1_2_7_109_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2010.01.010"},{"issue":"1","key":"e_1_2_7_110_1","first-page":"62","article-title":"Validity of nucleic acid purities monitored by 260nm\/280nm absorbance ratios","volume":"18","author":"Glasel J","year":"1995","journal-title":"Biotechniques"},{"key":"e_1_2_7_111_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2013.00056"},{"key":"e_1_2_7_112_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-15-395"},{"key":"e_1_2_7_113_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12867-015-0039-3"},{"key":"e_1_2_7_114_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2199-7-3"},{"key":"e_1_2_7_115_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-53892-x"},{"key":"e_1_2_7_116_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth908"},{"key":"e_1_2_7_117_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3014"},{"key":"e_1_2_7_118_1","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics11060964"},{"key":"e_1_2_7_119_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2010-11-10-r106"},{"key":"e_1_2_7_120_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.079558.108"},{"key":"e_1_2_7_121_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-12-293"},{"key":"e_1_2_7_122_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-11-94"},{"key":"e_1_2_7_123_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/19.2.185"},{"key":"e_1_2_7_124_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1226"},{"key":"e_1_2_7_125_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2013.157"},{"key":"e_1_2_7_126_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btt688"},{"key":"e_1_2_7_127_1","unstructured":"Stanford University.Current ENCODE experiment guidelines \u2013 ENCODE.https:\/\/www.encodeproject.org\/about\/experiment\u2010guidelines\/Accessed January 20 2025."},{"key":"e_1_2_7_128_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-13-484"},{"key":"e_1_2_7_129_1","doi-asserted-by":"publisher","DOI":"10.1261\/rna.046011.114"},{"key":"e_1_2_7_130_1","doi-asserted-by":"publisher","DOI":"10.1089\/cmb.2012.0283"},{"key":"e_1_2_7_131_1","unstructured":"RNA\u2010Seq Blog.RNA\u2010Seq blog: poll results. RNA\u2010Seq Blog.http:\/\/www.rna\u2010seqblog.com\/rna\u2010seq\u2010blog\u2010poll\u2010results\u201025\/. Accessed April 29 2024."},{"key":"e_1_2_7_132_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1105136"},{"key":"e_1_2_7_133_1","unstructured":"Stanford University.Small RNA\u2010seq data standards and processing pipeline.https:\/\/www.encodeproject.org\/data\u2010standards\/rna\u2010seq\/small\u2010rnas\/Accessed January 20 2025."},{"key":"e_1_2_7_134_1","doi-asserted-by":"publisher","DOI":"10.3402\/jev.v4.27493"},{"key":"e_1_2_7_135_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cels.2019.03.004"},{"key":"e_1_2_7_136_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2013.03.027"},{"key":"e_1_2_7_137_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkx836"},{"key":"e_1_2_7_138_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp616"},{"key":"e_1_2_7_139_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8"},{"key":"e_1_2_7_140_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2010-11-8-r86"},{"key":"e_1_2_7_141_1","unstructured":"GalaxyT.GalaxyHelp.https:\/\/help.galaxyproject.org\/. Accessed April 29 2024."},{"key":"e_1_2_7_142_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty338"},{"key":"e_1_2_7_143_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btx066"},{"key":"e_1_2_7_144_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12859-018-2047-z"},{"key":"e_1_2_7_145_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab268"},{"key":"e_1_2_7_146_1","doi-asserted-by":"publisher","DOI":"10.1093\/database\/bay004"},{"key":"e_1_2_7_147_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-016-0881-8"},{"key":"e_1_2_7_148_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.compbiomed.2020.104060"},{"key":"e_1_2_7_149_1","doi-asserted-by":"publisher","DOI":"10.3390\/ncrna9020018"},{"key":"e_1_2_7_150_1","unstructured":"ChuCP.How to analyze RNA\u2010Seq data? Find differentially expressed genes in your research.https:\/\/www.youtube.com\/watch?v=xh_wpWj0AzM. Accessed April 29 2024."},{"key":"e_1_2_7_151_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.21848"},{"key":"e_1_2_7_152_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2011.07.031"},{"key":"e_1_2_7_153_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2016.02.019"},{"key":"e_1_2_7_154_1","doi-asserted-by":"publisher","DOI":"10.1186\/jbiol104"},{"key":"e_1_2_7_155_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2484"},{"key":"e_1_2_7_156_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg3305"},{"key":"e_1_2_7_157_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1503"},{"key":"e_1_2_7_158_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1158441"},{"key":"e_1_2_7_159_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000834"},{"key":"e_1_2_7_160_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms15190"},{"key":"e_1_2_7_161_1","doi-asserted-by":"publisher","DOI":"10.3816\/CLC.2009.n.006"},{"key":"e_1_2_7_162_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkl1130"},{"key":"e_1_2_7_163_1","doi-asserted-by":"publisher","DOI":"10.1261\/rna.939908"},{"key":"e_1_2_7_164_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2016.03.025"},{"key":"e_1_2_7_165_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2002-3-7-research0034"},{"key":"e_1_2_7_166_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-0496"},{"key":"e_1_2_7_167_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_7_168_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.9.e45"},{"key":"e_1_2_7_169_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2009-10-6-r64"},{"key":"e_1_2_7_170_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg3198"},{"key":"e_1_2_7_171_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2007-8-2-r19"},{"key":"e_1_2_7_172_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-61779-427-8_18"},{"key":"e_1_2_7_173_1","unstructured":"CellCarta.Genomic data analysis.https:\/\/cellcarta.com\/genomic\u2010data\u2010analysis\/. Accessed October 11 2024."},{"key":"e_1_2_7_174_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2008.112797"},{"key":"e_1_2_7_175_1","unstructured":"RDML\u2009Consortium.MIQE: minimum information for publication of quantitative real\u2010time PCR experiments.https:\/\/rdml.org\/miqe.htmlAccessed\u2009January 20 2025."},{"key":"e_1_2_7_176_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkp056"},{"key":"e_1_2_7_177_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2633"},{"key":"e_1_2_7_178_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2019.03.008"},{"key":"e_1_2_7_179_1","doi-asserted-by":"publisher","DOI":"10.1080\/20013078.2018.1535750"},{"key":"e_1_2_7_180_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1004947"},{"key":"e_1_2_7_181_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12859-015-0637-6"},{"key":"e_1_2_7_182_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts635"}],"container-title":["Veterinary Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vcp.13412","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/vcp.13412","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vcp.13412","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T05:59:05Z","timestamp":1770962345000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vcp.13412"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,26]]},"references-count":181,"journal-issue":{"issue":"S2","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["10.1111\/vcp.13412"],"URL":"https:\/\/doi.org\/10.1111\/vcp.13412","archive":["Portico"],"relation":{},"ISSN":["0275-6382","1939-165X"],"issn-type":[{"value":"0275-6382","type":"print"},{"value":"1939-165X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,26]]},"assertion":[{"value":"2024-04-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-28","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}