{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,2]],"date-time":"2025-08-02T17:11:54Z","timestamp":1754154714840,"version":"3.41.2"},"reference-count":10,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,6,2]],"date-time":"2025-06-02T00:00:00Z","timestamp":1748822400000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007709","name":"Michigan State University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007709","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Pancreatic lipase assays are commonly utilized in the diagnostic approach to suspected pancreatitis in cats. The Vcheck fPL 2.0 assay is commercially available; however, analytical validation has yet to be reported in the peer\u2010reviewed literature.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To evaluate the analytic validity of a fluorescent immunoassay for the point\u2010of\u2010care quantification of feline pancreatic lipase immunoreactivity (Vcheck fPL 2.0 assay).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Linearity was assessed via dilutional parallelism. Intra\u2010 and interassay variability was assessed by calculating coefficients of variation (%CV) across replicates on the same day and across multiple days, respectively. Bilirubin, hemoglobin, and intralipid (intralipid 30% IV emulsion, VWR, Radnor, PA, USA) were utilized to evaluate the potential effects of interfering substances on the assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Linearity was sub\u2010optimal, with 12\/15 diluted runs having an O\/E ratio outside of the 80\u2013120% target range. The mean %CV was 10.2% for intra\u2010assay variability and 16.3% for interassay variability. Interfering substances had no significant effect on the results of the Vcheck fPL 2.0 assay (<jats:italic>P<\/jats:italic>\u2009=\u20090.888).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The Vcheck fPL 2.0 appears to have poor linearity and suboptimal precision, limiting the accurate interpretation of results. Additional assay optimization should be considered prior to routine clinical use.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vcp.70018","type":"journal-article","created":{"date-parts":[[2025,6,2]],"date-time":"2025-06-02T08:18:17Z","timestamp":1748852297000},"page":"154-157","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Analytical Validation of an Automated Point\u2010of\u2010Care Immunoassay for the Measurement of Feline Pancreatic Lipase Immunoreactivity Concentration (Vcheck <scp>fPL<\/scp> 2.0)"],"prefix":"10.1111","volume":"54","author":[{"given":"Lisa","family":"Wetzel","sequence":"first","affiliation":[{"name":"Department of Small Animal Clinical Sciences College of Veterinary Medicine, Michigan State University  East Lansing Michigan USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joerg M.","family":"Steiner","sequence":"additional","affiliation":[{"name":"Gastrointestinal Laboratory, Department of Small Animal Clinical Sciences College of Veterinary Medicine and Biomedical Sciences, Texas A&amp;M University  College Station Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0236-2307","authenticated-orcid":false,"given":"Harry","family":"Cridge","sequence":"additional","affiliation":[{"name":"Department of Small Animal Clinical Sciences College of Veterinary Medicine, Michigan State University  East Lansing Michigan USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,6,2]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.16053"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvim.16292"},{"issue":"3","key":"e_1_2_7_4_1","first-page":"189","article-title":"A Critical Evaluation of Serum Lipase and Amylase as Diagnostic Tests for Acute Pancreatitis","volume":"2","author":"Jasdanwala S.","year":"2015","journal-title":"Integrative Molecular Medicine"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplegacy.1932.100.2.266"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.2460\/javma.22.03.0144"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.12906"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.13283"},{"key":"e_1_2_7_9_1","unstructured":"Bionote \u201cBionote: Vcheck fPL 2.0 \u201daccessed October 21 2024 https:\/\/www.bionote.com\/_files\/ugd\/1a98f3_dd68704131c44fc69d656706617f221d.pdf."},{"key":"e_1_2_7_10_1","unstructured":"US Department of Health & Human Services Food and Drug Administration Center for Drug Evaluation and Research (CDER) Center for Veterinary Medicine (CVM) \u201cGuidance for Industry Bioanalytical Method Validation \u201daccessed October 21 2024 https:\/\/www.fda.gov\/regulatory\u2010information\/search\u2010fda\u2010guidance\u2010documents\/bioanalytical\u2010method\u2010validation\u2010guidance\u2010industry."},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2004.040154"}],"container-title":["Veterinary Clinical Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/vcp.70018","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,24]],"date-time":"2025-07-24T17:46:27Z","timestamp":1753379187000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/vcp.70018"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6]]},"references-count":10,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2025,6]]}},"alternative-id":["10.1111\/vcp.70018"],"URL":"https:\/\/doi.org\/10.1111\/vcp.70018","archive":["Portico"],"relation":{},"ISSN":["0275-6382","1939-165X"],"issn-type":[{"type":"print","value":"0275-6382"},{"type":"electronic","value":"1939-165X"}],"subject":[],"published":{"date-parts":[[2025,6]]},"assertion":[{"value":"2024-12-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}