{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,18]],"date-time":"2025-09-18T18:38:39Z","timestamp":1758220719675,"version":"3.44.0"},"reference-count":28,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T00:00:00Z","timestamp":1751414400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100001250","name":"Morris Animal Foundation","doi-asserted-by":"publisher","award":["D16ZO\u2010829"],"award-info":[{"award-number":["D16ZO\u2010829"]}],"id":[{"id":"10.13039\/100001250","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Clinical Pathol"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Reference intervals (<jats:styled-content style=\"fixed-case\">RIs<\/jats:styled-content>) are an essential tool for assessment of clinical pathology data of animals, and are particularly important for monitoring the health status of free\u2010ranging and captive wildlife, such as koalas (<jats:styled-content style=\"fixed-case\"><jats:italic>Phascolarctos cinereus<\/jats:italic><\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>The purpose of this study was to: (1) provide comprehensive serum biochemistry reference intervals based on clinically healthy South Australian koalas from two populations, Mount Lofty Ranges (MLR) and Kangaroo Island (KI); and (2) identify any factors that can affect biochemical analytes, including koala retrovirus (KoRV) and <jats:styled-content style=\"fixed-case\"><jats:italic>Chlamydia pecorum<\/jats:italic><\/jats:styled-content> subclinical infection status, age, sex, and population.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Serum biochemistry analytes were determined in 206 clinically healthy South Australian koalas caught from the wild in 2016 and 2018 using a Cobas 8000 Chemistry Analyzer and analyzed using Reference Value Advisor and SPSS v28 Statistical software.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Biochemical reference intervals were established. Also, clinically and statistically significant differences in analytes were found based on age for alkaline phosphatase and phosphate, and albumin: globulin ratio, globulins, and total protein, most likely associated with physiological bone growth and immunological development, respectively, as observed in other species. Statistically significant differences between animals subclinically positive for KoRV and <jats:styled-content style=\"fixed-case\"><jats:italic>Chlamydia pecorum<\/jats:italic><\/jats:styled-content>, were found for glucose and gamma glutamyl transferase respectively; however, these were marginal, and their reference intervals were similar.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study is the first to describe serum biochemical reference intervals for clinically healthy South Australian koalas of known <jats:italic>Chlamydia<\/jats:italic> and KoRV infection status. It represents an important tool to assist health assessments of koalas by veterinarians, as well as research and population monitoring.<\/jats:p><\/jats:sec>","DOI":"10.1111\/vcp.70024","type":"journal-article","created":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T05:04:56Z","timestamp":1751432696000},"page":"300-308","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Biochemical Reference Intervals of Free\u2010Ranging Koalas (<scp><i>Phascolarctos cinereus<\/i><\/scp>) in South Australia"],"prefix":"10.1111","volume":"54","author":[{"given":"Mar\u00eda B.","family":"Font","sequence":"first","affiliation":[{"name":"Faculty of Exact and Natural Sciences, University of Buenos Aires  Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7271-2937","authenticated-orcid":false,"given":"Lucy","family":"Woolford","sequence":"additional","affiliation":[{"name":"School of Animal and Veterinary Sciences Faculty of Science, Engineering and Technology, University of Adelaide  Adelaide South Australia Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1754-500X","authenticated-orcid":false,"given":"Sue","family":"Jaensch","sequence":"additional","affiliation":[{"name":"Vetnostics Laboratory  North Ryde New South Wales Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5346-703X","authenticated-orcid":false,"given":"Doug","family":"Hayward","sequence":"additional","affiliation":[{"name":"Vetnostics Laboratory  North Ryde New South Wales Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michelle","family":"Hebart","sequence":"additional","affiliation":[{"name":"School of Animal and Veterinary Sciences Faculty of Science, Engineering and Technology, University of Adelaide  Adelaide South Australia Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emily","family":"Dean","sequence":"additional","affiliation":[{"name":"School of Animal and Veterinary Sciences Faculty of Science, Engineering and Technology, University of Adelaide  Adelaide South Australia Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wayne S. 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